干旱区生态环境知识资源中心(Arid): Ultrastructural and flow cytometric analyses of lipid accumulation in microalgae

Arid

DOI	10.2172/6757339
报告编号	SERI/STR-231-3089
来源ID	OSTI_ID: 6757339
	Ultrastructural and flow cytometric analyses of lipid accumulation in microalgae
	Solomon, J.A.; Hand, R.E. Jr.; Mann, R.C.
英文摘要	Lipid accumulation in three species of microalgae was investigated with flow cytometry (FCM) and transmission electron microscopy (TEM). Previous studies using batch cultures of a algae have led to the assumption that lipid accumulation in microalgae is a gradual process requiring at least several days for completion. However, FCM reveals, through changes in the chlorophyll:lipid ratio, that the time span required for individual cells to change metabolic state is short. Simultaneous FCM measurements of chlorophyll and nile red (neutral lipid) fluorescence in individual cells of nitrogen-deficient Isochrysis populations revealed a bimodal population distribution as one stage in the lipid accumulation process. The fact that two discrete populations exist, with few cells in an intermediate stage, suggests rapid response to a liqid trigger. Interpretations of light and electron microscopic observations are consistent with this hypothesis. The time required for an entire population to achieve maximum lipid content is considerably longer than that required for a single cell, due to the variation in response time among cells. In this study high lipid cultures were sometimes obtained by using FCM to separate high lipid cells from the remainder of the population. FCM holds much promise for strain enhancement but considerable developmental work, directed at providing more consistent results, remains to be done. 8 refs., 35 figs.
出版年	1986
报告类型	Technical Report
语种	英语
国家	美国
来源学科分类	59 BASIC BIOLOGICAL SCIENCES ; 09 BIOMASS FUELS ; ALGAE ; ULTRASTRUCTURAL CHANGES ; LIPIDS ; BIOSYNTHESIS ; CELL FLOW SYSTEMS ; TRANSMISSION ELECTRON MICROSCOPY ; ELECTRON MICROSCOPY ; MICROSCOPY ; MORPHOLOGICAL CHANGES ; ORGANIC COMPOUNDS ; PLANTS ; SYNTHESIS ALGAE ; AQUATIC SPECIES PROGRAM ; LIPIDS ; ELECTRON MICROSCOPY ; CELL FLOW SYSTEMS 550700* -- Microbiology ; 140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
URL	http://www.osti.gov/scitech/servlets/purl/6757339
资源类型	科技报告
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/263885
推荐引用方式 GB/T 7714	Solomon, J.A.,Hand, R.E. Jr.,Mann, R.C.. Ultrastructural and flow cytometric analyses of lipid accumulation in microalgae,1986.