Knowledge Resource Center for Ecological Environment in Arid Area
| Presence of Wolbachia, A Potential Biocontrol Agent: Screening for Vertebrate Blood Meal Source and West Nile Virus in Mosquitoes in the North Texas Region | |
| Adiji;Olubu Adeoye | |
| 出版年 | 2016 |
| 学位授予单位 | University of North Texas |
| 英文摘要 | West Nile virus (WNV) is a geographically endemic mosquito-borne flavivirus that has spread across the United States infecting birds, mosquitos, humans, horses and other mammals. The wide spread nature of this virus is due to the ability of the mosquito vector to persist in broad, ecological diverse environments across the United States. In this study, mosquito populations in North Texas region were sampled for detection of Wolbachia, blood meal source, and WNV. The ultimate goal of this study was to examine the potential of a biocontrol agent, Wolbachia sp. that colonizes the hindgut of various insects, including mosquitos, as a natural means to interrupt virus transmission from mosquitos to other hosts, including humans. In Australia, Wolbachia sp. from fruit flies (Drosophila melanogaster) have been successfully used to block transmission of a similar pathogenic virus from mosquitos responsible for transmission of Dengue fever. Here, mosquitoes were collected using CDC style Gravid Traps in Denton, Texas, from October 2012 through September 2014. Collected mosquitoes were identified, sexed, and categorized as to the amount of host blood in their alimentary system using a Zeiss Axio Zoom microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY). Culex quinquefaciatus was the dominant blood engorged species collected. Smaller populations of Culex tarsalis and Aedes albopictus, another known vector for WNV were also collected. Mosquito larva were also collected from the UNT water research field station and reared to adults. Cx. tarsalis was the dominant mosquito taken from this habitat. Samples of Cx. quinquefasciatus, Cx. tarsalis and A. albopictus were analyzed for Wolbachia sp. and to identify host blood in the mosquito alimentary system. Total DNA extraction from the pool of mosquito samples was by both commercially available DNA extraction kits (Qiagen, Valencia, CA) and salt extraction technique. Polymerase chain reaction (PCR) was used to amplify and identify Wolbachia sp. 16SrDNA and mitochondrial DNA from vertebrate blood. The maternally inherited endosymbiont, Wolbachia, were found to be uniformly distributed across the mosquitoes sampled in this study. Blood meal analysis by PCR showed that Cx. quinquefaciatus fed more on birds than on mammalian blood sources based on the previously developed primers used in this study. |
| 英文关键词 | Wolbachia Mosquito West Nile Virus Culex quinquefaciatus |
| 语种 | 英语 |
| URL | https://digital.library.unt.edu/ark:/67531/metadc862878 |
| 来源机构 | University of North Texas |
| 资源类型 | 学位论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/250049 |
| 推荐引用方式 GB/T 7714 | Adiji;Olubu Adeoye. Presence of Wolbachia, A Potential Biocontrol Agent: Screening for Vertebrate Blood Meal Source and West Nile Virus in Mosquitoes in the North Texas Region[D]. University of North Texas,2016. |
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