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| The Identification of Alkaloid Pathway Genes from Non-Model Plant Species in the Amaryllidaceae | |
| Kilgore, Matthew Benjamin | |
| 出版年 | 2015 |
| 学位类型 | 博士 |
| 导师 | Kutchan, Toni M. |
| 学位授予单位 | Washington University in St. Louis |
| 英文摘要 | Secondary metabolites are often restricted in their distribution to different groups of organisms. For this reason, attempts to study these often useful and interesting products of metabolism require an ability to work in a diversity of non-model species. Methods for gene discovery with low investment and high efficiency are needed to effectively identify the biosynthetic genes in these diverse pathways. During this work, a workflow for efficiently identifying biosynthetic genes was developed and applied to Amaryllidaceae alkaloid biosynthesis. Genes discovered during this work include a norbelladine 4’- O-methyltransferase, a cytochrome P450 capable of phenol-phenol coupling 4’-O-methylnorbelladine to noroxomaritidine, and a short-chain dehydrogenase/reductase capable of forming norbelladine from tyramine and 3,4-dihydroxybenzaldehyde. These enzymatic discoveries support the future application of this workflow to other biosynthetic pathways and organisms. |
| 英文关键词 | Amaryllidaceae Amaryllidaceae alkaloids Cytochrome P450 Galanthus Narcissus Norbelladine |
| 语种 | 英语 |
| 国家 | United States |
| 来源学科分类 | Plant sciences; Biochemistry |
| URL | https://pqdtopen.proquest.com/doc/1753917088.html?FMT=AI |
| 来源机构 | Washington University in St. Louis |
| 资源类型 | 学位论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/249009 |
| 推荐引用方式 GB/T 7714 | Kilgore, Matthew Benjamin. The Identification of Alkaloid Pathway Genes from Non-Model Plant Species in the Amaryllidaceae[D]. Washington University in St. Louis,2015. |
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