Knowledge Resource Center for Ecological Environment in Arid Area
| Electron microscopy: The mechanism of minus-end directed kinesins, the assembly of 30S ribosomal subunits, and computer vision applied to the automation of tilted data collection | |
| Yoshioka, Craig K | |
| 出版年 | 2008 |
| 学位类型 | 博士 |
| 学位授予单位 | The Scripps Research Institute |
| 英文摘要 | This work begins with the structural study of a molecular motor, Ncd, whose mechanism of movement toward the minus-end of microtubules was unknown. The work was done using older, manual EM methods, and upon completion, the author had a new found respect for the value of automated technologies. With this new interest, we began exploring ways to improve automated EM techniques, specifically through the application of methods from the field of Computer Vision. While doing so, we implemented a way to collect tilted data in an automated fashion, which opened the door for some new and interesting possibilities for examining structurally heterogeneous samples. Having done this, and various other improvements, we then returned to the study of biological systems to find one that could make especial use of our newly developed methods. In particular, we found that such a system is visualizing the assembly of 30S E. coli small ribosomal subunits. Our initial characterization and observations have shown that even this dynamic system is now open to study using structural methods. |
| 英文关键词 | Computer vision Data collection Kinesins Ribosomal subunits Ribosome assembly |
| 语种 | 英语 |
| 国家 | United States |
| 来源学科分类 | Biophysics; Computer science |
| URL | https://pqdtopen.proquest.com/doc/304813387.html?FMT=AI |
| 来源机构 | Scripps Research Institute |
| 资源类型 | 学位论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/244237 |
| 推荐引用方式 GB/T 7714 | Yoshioka, Craig K. Electron microscopy: The mechanism of minus-end directed kinesins, the assembly of 30S ribosomal subunits, and computer vision applied to the automation of tilted data collection[D]. The Scripps Research Institute,2008. |
| 条目包含的文件 | 条目无相关文件。 | |||||
| 个性服务 |
| 推荐该条目 |
| 保存到收藏夹 |
| 导出为Endnote文件 |
| 谷歌学术 |
| 谷歌学术中相似的文章 |
| [Yoshioka, Craig K]的文章 |
| 百度学术 |
| 百度学术中相似的文章 |
| [Yoshioka, Craig K]的文章 |
| 必应学术 |
| 必应学术中相似的文章 |
| [Yoshioka, Craig K]的文章 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
