干旱区生态环境知识资源中心(Arid): 通过式固相萃取净化-超高效液相色谱-串联质谱法测定鱼肉中7种非选择性环氧化酶抑制药物残留

Arid

	通过式固相萃取净化-超高效液相色谱-串联质谱法测定鱼肉中7种非选择性环氧化酶抑制药物残留
其他题名	Determination of 7 non-selective cyclooxygenase inhibitors in fish by ultra-performance liquid chromatography-tandem mass spectrometry with pass-through solid phase extraction
	励炯; 金朦娜; 邱红钰
来源期刊	中国食品卫生杂志
ISSN	1004-8456
出版年	2019
卷号	31 期号:2 页码:116-121
中文摘要	目的建立并优化鱼肉中7种非选择性环氧化酶抑制药物残留的通过式固相萃取净化-超高效液相色谱-串联质谱检测方法。方法样品用提取剂(含90%乙腈水溶液和5%甲酸)提取后,提取液经Oasis PRiME HLB固相萃取柱净化,Waters CORTECS UPLC C_(18)色谱柱(100 mm*2. 1 mm,1. 6 mum)分离,以含0. 1%甲酸的水溶液和含0. 1%甲酸的乙腈溶液为流动相梯度洗脱,电喷雾电离,多反应监测(MRM)定性定量分析水杨酸、布洛芬、双氯芬酸钠、吲哚美辛、吡罗昔康、萘普生和保泰松7种成分。结果7种化合物线性相关性良好,相关系数(r~2) ≥0. 995 2;方法回收率用三个浓度进行添加试验,回收率为80. 3 %~ 95. 5%;定量限为2 ~ 10 mug /kg。结论本方法前处理简单、回收率高、重现性好,可用于检测鱼肉中7种非选择性环氧化酶抑制药物的残留。
英文摘要	Objective An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS /MS) with pass-through solid phase extraction method was developed to determine 7 non-selective cyclooxygenase inhibitors in fish. Methods Samples were extracted with extractant containing 90% acetonitrile and 5% formic acid,and then purified with Oasis PRiME HLB columns. The analysis was performed with Waters CORTECS UPLC C_(18) (100 mm*2. 1 mm,1. 6 mum) column. The mobile phase was consisted of 0. 1% formic acid aqueous solution and acetonitrile containing 0. 1% formic acid by gradient elution,and multiple reaction monitoring (MRM) mode with electrospray ionization was used to analyse salicylic acid,piroxicam,naproxen,indomethacin,diclofenac sodium,ibuprofen and phenylbutazone. The effect of the dosages of extractant,formic acid and acetonitrile in cleaning-up agent was studied. The main influence factors and analytical conditions were modified. Results Seven linear calibration curves were obtained with correlation coefficients (r~2) ≥0. 995 2. The recoveries at three concentrations ranged from 80. 3%-95. 5%. The limits of quantifications were from 2 to 10 mug /kg. Conclusion The method has the advantages of simple pretreatment,high recovery and good reproducibility which is suitable for the determination of 7 non-selective cyclooxygenase inhibitors in fish.
中文关键词	超高效液相色谱-串联质谱 ; 通过式固相萃取 ; 鱼肉 ; 非选择性环氧化酶抑制药物
英文关键词	Ultra-performance liquid chromatography-tandem mass spectrometry pass-through solid phase extraction fish non-selective cyclooxygenase inhibitor
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PUBLIC ENVIRONMENTAL OCCUPATIONAL HEALTH
WOS研究方向	Public, Environmental & Occupational Health
CSCD记录号	CSCD:6538014
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239821
作者单位	杭州市食品药品检验研究院, 杭州, 浙江 310017, 中国
推荐引用方式 GB/T 7714	励炯,金朦娜,邱红钰. 通过式固相萃取净化-超高效液相色谱-串联质谱法测定鱼肉中7种非选择性环氧化酶抑制药物残留[J],2019,31(2):116-121.
APA	励炯,金朦娜,&邱红钰.(2019).通过式固相萃取净化-超高效液相色谱-串联质谱法测定鱼肉中7种非选择性环氧化酶抑制药物残留.中国食品卫生杂志,31(2),116-121.
MLA	励炯,et al."通过式固相萃取净化-超高效液相色谱-串联质谱法测定鱼肉中7种非选择性环氧化酶抑制药物残留".中国食品卫生杂志 31.2(2019):116-121.