干旱区生态环境知识资源中心(Arid): 不同生态条件下棉花赤霉素合成代谢途径关键酶基因差异表达分析

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	不同生态条件下棉花赤霉素合成代谢途径关键酶基因差异表达分析
其他题名	Differential expression of the key enzyme of gibberellin biosynthesis pathway in cotton under different ecological conditions
	石建斌; 王宁; 周红; 许庆华; 乔文青; 严根土
来源期刊	中国农业大学学报
ISSN	1007-4333
出版年	2019
卷号	24 期号:1 页码:6-13
中文摘要	为揭示棉花植株体内赤霉素合成途径关键酶基因表达量与不同组织及环境条件的关系,以中棉所49(CCRI49)和高脚棉"(396289)为材料,利用实时荧光定量PCR方法中的相对定量,以UBQ7为内参基因,对新疆维吾尔自治区阿拉尔和河南省安阳,2种不同生态环境下的CCRI49和396289不同组织内赤霉素合成途径相关基因的表达量进行检测。结果显示,在阿拉尔地区生长条件下,CCRI49果枝始生节位相比于安阳地区平均降低2.0,高脚棉平均降低2.6。在安阳地区,CCRI49和396289中赤霉素合成途径各关键酶基因在不同组织内的表达趋势基本一致,茎组织中各相关基因表达量高,为植株生长提供充足的活性赤霉素;在新疆阿拉尔地区,CCRI49和396289主要表现为赤霉素合成早期阶段CPS1、KS和KO等基因的表达量下调,GA2ox1表达量上调,以减少活性赤霉素的合成。结果表明,棉花植株赤霉素合成代谢途径关键酶基因的表达量发生改变,从而调控植株的生长发育来适应新疆干旱和盐渍化的生长环境。
英文摘要	To explore the relationship between the key enzyme gene expression of gibberellin synthesis pathway and different tissues or environmental condition, cotton CCRI49 and 396289 were selected as material, and UBQ7 was selected as reference gene, Relative quantification real-time PCR was developed to determine the gibberellin key gene expression of different cotton organizations under two kinds of ecological environment. The results showed that: compared with Anyang, the average node of first fruiting/sympodial branch of CCRI49 and 396289 were repectively decreased by 2.0 and 2.6 under the growth condition of Alar. In Anyang region, the expression trends of gibberellin related gene were basically identical in tissues, but the expression level in stem was higher, which was beneficial for the growth of plants. In Alar region, the CPS1, KS, and KO gene expressions were significantly down-regulated in CCRI49 and 396289, while GA2ox1 gene expression was up-regulated. The down-regulation of CPS1, KS and KO reduced the synthesis rate of gibberellin, and the up-regulation of GA2ox1 decomposed the active gibberellin. To adapt to the arid and salinized growth environment of Xinjiang, the cotton plant regulate its growth and development by changing the expression of the key enzyme gene of gibberellin in the plant.
中文关键词	棉花 ; 赤霉素 ; 环境条件 ; 基因表达量
英文关键词	cotton gibberellin environmental condition gene expression
语种	中文
国家	中国
收录类别	CSCD
WOS类目	GENETICS HEREDITY
WOS研究方向	Genetics & Heredity
CSCD记录号	CSCD:6410735
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239744
作者单位	中国农业科学院棉花研究所, 棉花生物学国家重点实验室, 安阳, 河南 455000, 中国
推荐引用方式 GB/T 7714	石建斌,王宁,周红,等. 不同生态条件下棉花赤霉素合成代谢途径关键酶基因差异表达分析[J],2019,24(1):6-13.
APA	石建斌,王宁,周红,许庆华,乔文青,&严根土.(2019).不同生态条件下棉花赤霉素合成代谢途径关键酶基因差异表达分析.中国农业大学学报,24(1),6-13.
MLA	石建斌,et al."不同生态条件下棉花赤霉素合成代谢途径关键酶基因差异表达分析".中国农业大学学报 24.1(2019):6-13.