干旱区生态环境知识资源中心(Arid): 固相萃取-超高效液相色谱-串联质谱法同时测定地表水中9种性激素

Arid

	固相萃取-超高效液相色谱-串联质谱法同时测定地表水中9种性激素
其他题名	Simultaneous Determination of 9 Hormones in Surface Water by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid-Phase Extraction
	杨金泉 1; 贺小敏 1; 施敏芳 1; 朱友林 2
来源期刊	中国环境监测
ISSN	1002-6002
出版年	2019
卷号	35 期号:3 页码:19-27
中文摘要	建立了地表水中9种性激素的固相萃取-超高效液相色谱-串联质谱检测方法。利用HLB固相萃取柱富集水体中痕量性激素,用甲醇洗脱并浓缩,再以1 mmol /L氟化铵-乙腈为流动相,经C18柱分离,采用电喷雾离子源、质谱多反应监测模式,内标法定量,实现了地表水中9种性激素的同时检测。方法检出限为0.1~ 1.8 ng /L,在低、中、高3个加标水平下,性激素的平均回收率为69.6%~ 115.0%,相对标准偏差为3.2% ~ 17.7%。该方法灵敏度高,定性准确,操作简单高效,适用于地表水中9种性激素的定性定量分析。
英文摘要	An analytical method was developed for simultaneous determination of 9 hormones in surface water samples by solidphase extraction and ultra performance liquid chromatography-tandem mass spectrometry ( SPE-UPLC-MS /MS). The sample was enriched by Oasis HLB solid phase extraction cartridge, extracted with methanol and concentrated by nitrogen. After chromatographic separation on an C18 column with 1 mmol /L NH4F aqueous-acetonitrile as the mobile phase,the target compounds were analyzed under the multiple reaction monitoring( MRM) mode and quantitated by internal standard method. The limits of detection for 9 hormones were in the range of 0.1-1.8 ng /L. The mean recoveries at three spiked levels ranged from 69.6% to 115.0%,with relative standard deviations of 3.2%-17.7%. The method was proved to be sensitive,accurate and efficient,and it was suitable for qualitative and quantitative analysis of 9 hormones in surface water.
中文关键词	液相色谱-串联质谱法 ; 性激素 ; 地表水 ; 固相萃取
英文关键词	LC-MS /MS hormone surface water solid-phase extraction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENVIRONMENTAL SCIENCES
WOS研究方向	Environmental Sciences & Ecology
CSCD记录号	CSCD:6519479
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239734
作者单位	1.湖北省环境监测中心站, 武汉, 湖北 430072, 中国; 2.长江大学, 武汉, 湖北 430000, 中国
推荐引用方式 GB/T 7714	杨金泉,贺小敏,施敏芳,等. 固相萃取-超高效液相色谱-串联质谱法同时测定地表水中9种性激素[J],2019,35(3):19-27.
APA	杨金泉,贺小敏,施敏芳,&朱友林.(2019).固相萃取-超高效液相色谱-串联质谱法同时测定地表水中9种性激素.中国环境监测,35(3),19-27.
MLA	杨金泉,et al."固相萃取-超高效液相色谱-串联质谱法同时测定地表水中9种性激素".中国环境监测 35.3(2019):19-27.