干旱区生态环境知识资源中心(Arid): 固相萃取净化-UPLC-MS/MS法同时测定中成药及保健食品中添加的17个抗过敏类化学成分

Arid

	固相萃取净化-UPLC-MS/MS法同时测定中成药及保健食品中添加的17个抗过敏类化学成分
其他题名	Determination of 17 anti-allergic components in Chinese patent medicine and health foods by UPLC-MS/MS coupled with solid phase extraction
	彭彦; 杨直; 金朦娜; 林丽琴
来源期刊	药物分析杂志
ISSN	0254-1793
出版年	2019
卷号	39 期号:6 页码:1115-1126
中文摘要	目的:建立了固相萃取柱净化,超高效液相色谱-三重四极杆串联质谱联用(UPLC-MS/MS)测定中成药及保健食品中添加的17个抗过敏类化学成分的方法。方法:样品用甲醇提取后,与2%甲酸溶液以1∶9的比例混合,经Oasis MCX固相萃取柱净化,采用Waters ACQUITY UPLC BEH C_(18)(2.1 mm* 100 mm,1.8 mum)分离16个化学成分,以甲醇-0.1%氨水为流动相进行梯度洗脱;大赛璐CHIRALPAK AGP柱(150 mm*4 mm,5 mum)分离2个手性化学成分(西替利嗪与左西替利嗪),以甲醇-10 mmol·L~(-1)乙酸铵(v/v)溶液为流动相等度洗脱。在电喷雾离子源(ESI)正负离子模式下电离,多反应监测(MRM)模式下测定。结果:17个抗过敏化学成分在相应的测定范围内线性关系良好,相关系数(r)在0.995 7~1.000之间,在3个加标水平下,加标回收率范围为84.3%~112.9%,RSD(n=6)为0.43%~3.7%,各化学成分的检测下限在0.14~0.91 mg·kg~(-1)之间。收集的32批次样品中5批次检出氯苯那敏,含量在0.015~3.86 mg·g~(-1)之间、 1批次检出西替利嗪,含量为1.68 mg·g~(-1),1批次检出左西替利嗪,含量为1.93 mg·g~(-1),1批次检出苯海拉明,含量为1.30 mg·g~(-1)。结论:该方法简便,选择性好,灵敏度高,可用于中成药和保健食品中多组分抗过敏类化学成分的同时定量测定。
英文摘要	Objective:To develop a method based on SPE-UPLC-MS/MS(solid phase extraction-ultraperformance liquid chromatography-tandem mass spectrometry)for determination of 17 anti-allergic chemical components in Chinese patent medicine and health foods.Methods:Samples were extracted with methanol and then mixed with 2% formic acid solution at a ratio of 1∶9 in volume before purification with an Oasis MCX column.16 componewts were separated on an ACQUITY UPLC BEH C_(18)(2.1 mm100 mm,1.8 mum)column with 0.1% ammonia-methanol as mobile phases by gradient elution.A CHIRALPAK AGP(150 mm4 mm, 5 mum)column and gradient elution of 10 mmol·L~(-1) ammonium acetate and methanol were applied to separate two kinds of chiral components(cetirizine and levocetirizine).Detection was carried out by positive and negative electrospray ionization(ESI)mass spectrometer in multiple reaction monitor(MRM)mode.Results:All the 17 anti-allergic chemical components showed good linearity in their reasonable ranges(r=0.995 7-1.000).The average recoveries of all the componewts at three spiked levels were in the range of 84.3%-112.9% with RSDs(n=6) within 0.43%-3.7%.The LODs were all in the range of 0.14-0.91 mg·kg~(-1).Chlorpheniramine was found in 5 of the 32 tested samples,the contents were in range of 0.015-3.86 mg·g~(-1).Cetirizine and levocetirizine were found in 1 batch(cetirizine:1.68 mg·g~(-1),levocetirizine:1.93 mg·g~(-1)).In another sample,diphenhydramine was detected (1.30 mg·g~(-1)).Conclusion:With the advantages of rapidness,simplicity,good selectivity and high sensitivity, the method can be used in determination of anti-allergic compounds in Chinese patent medicine and health foods.
中文关键词	固相萃取 ; 液相色谱- 质谱联用法 ; 抗过敏类 ; 中成药 ; 保健食品
英文关键词	solid phase extraction(SPE) ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) anti-allergic Chinese patent medicine health food
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:6556831
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239655
作者单位	杭州市食品药品检验研究院, 杭州, 浙江 310000, 中国
推荐引用方式 GB/T 7714	彭彦,杨直,金朦娜,等. 固相萃取净化-UPLC-MS/MS法同时测定中成药及保健食品中添加的17个抗过敏类化学成分[J],2019,39(6):1115-1126.
APA	彭彦,杨直,金朦娜,&林丽琴.(2019).固相萃取净化-UPLC-MS/MS法同时测定中成药及保健食品中添加的17个抗过敏类化学成分.药物分析杂志,39(6),1115-1126.
MLA	彭彦,et al."固相萃取净化-UPLC-MS/MS法同时测定中成药及保健食品中添加的17个抗过敏类化学成分".药物分析杂志 39.6(2019):1115-1126.