干旱区生态环境知识资源中心(Arid): 石林石漠化地区可培养石生真菌多样性研究

Arid

	石林石漠化地区可培养石生真菌多样性研究
其他题名	Study on Diversity of Cultivable Rock-inhabiting Fungi in Shilin Rocky Desertification Area
	陈晓溪; 熊忠平; 李选文; 聂中良; 张珊; 凌溪; 熊智
来源期刊	西南农业学报
ISSN	1001-4829
出版年	2019
卷号	32 期号:6 页码:1397-1403
中文摘要	【目的】为开发利用石林石漠化地区石生真菌资源、探索石生真菌对碳酸盐岩风化及矿化作用提供理论基础。【方法】实验采用PDA和MEA培养基对采集自石林石漠化地区的40份岩石样品进行石生真菌的分离纯化,并观察分离得到的石生真菌的形态显微结构、分析rDNA-ITS序列同源性。【结果】从40份岩石样品中共分离获得68株石生真菌,分别隶属于2门5纲7目8科8属12种。其中31株镰刀菌属(Fusarium) 、9株毛壳菌属(Chaetomium) 、7株曲霉属(Aspergillus) 、6株葡萄座腔菌属(Botryosphaeria) 、5株青霉属(Penicillium) 、4株粪壳菌属(Sordaria) 、3株毛霉属(Mucor) 、3株被孢霉属(Mortierella Coemans) 。镰刀菌属、毛壳菌属、曲霉属具较高相对分离率,分别为45. 58 %、13. 23 %、10. 29 %。【结论】综合两种培养基,石生真菌的Shannon-Weiner多样性指数为2. 41922。石林石漠化地区石生真菌多样性丰富。
英文摘要	【Objective】To provide a theoretical basis for the development and utilization of rock-inhabiting fungi resources in Shilin rocky desertification area and explore the weathering and mineralization of carbonaceous by rock-inhabiting fungi.【Method】The PDA and MEA medium were used to separate and purify the rock-inhabiting fungi from 40 rock samples collected from the Shilin rocky desertification area. The morphology of the isolated rock-inhabiting fungi was observed and the rDNA-ITS sequence homology was analyzed. 【Result】A total of 68 rock-inhabiting fungi were isolated from 40 rock samples,belonging to 2 phylum,5 classes,7 orders,8 families,8 genera and 12 species. Among them,31 strains of Fusarium,9 strains of Chaetomium,7 strains of Aspergillus,6 strains of Botryosphaeria,5 strains of Penicillium, 4 strains of Sordaria,3 strains of Mucor,and 3 strains of Mortierella Coemans. Fusarium,Chaetomium,and Aspergillus had high relative separation rates,which were 45. 58 %,13. 23 %,and 10. 29 %,respectively. 【Conclusion】The Shannon-Weiner diversity index of the rock-inhabiting fungi was 2. 41922. The diversity of rock-inhabiting fungi is rich in Shilin rocky desertification area.
中文关键词	石林 ; 石漠化 ; 石生真菌 ; 可培养 ; 多样性
英文关键词	Shilin Rocky desertification Rock-inhabiting fungi Cultivable Diversity
语种	中文
国家	中国
收录类别	CSCD
WOS类目	GEOLOGY
WOS研究方向	Geology
CSCD记录号	CSCD:6537175
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239630
作者单位	西南林业大学生命科学学院, 昆明, 云南 650224, 中国
推荐引用方式 GB/T 7714	陈晓溪,熊忠平,李选文,等. 石林石漠化地区可培养石生真菌多样性研究[J],2019,32(6):1397-1403.
APA	陈晓溪.,熊忠平.,李选文.,聂中良.,张珊.,...&熊智.(2019).石林石漠化地区可培养石生真菌多样性研究.西南农业学报,32(6),1397-1403.
MLA	陈晓溪,et al."石林石漠化地区可培养石生真菌多样性研究".西南农业学报 32.6(2019):1397-1403.