干旱区生态环境知识资源中心(Arid): 亲水作用色谱-串联质谱法测定蜂蜜中链霉素和双氢链霉素残留

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	亲水作用色谱-串联质谱法测定蜂蜜中链霉素和双氢链霉素残留
其他题名	Determination of streptomycin and dihydrostreptomycin residues in honey by hydrophilic interaction liquid chromatography-tandem mass spectrometry
	魏莉莉; 薛霞; 刘艳明; 孙立臻; 程志; 宿书芳; 赵寅
来源期刊	色谱
ISSN	1000-8713
出版年	2019
卷号	37 期号:7 页码:735-741
中文摘要	建立了亲水作用色谱-串联质谱(HILIC-MS/MS)测定蜂蜜中链霉素和双氢链霉素的分析方法。样品中链霉素和双氢链霉素经20 g/L三氯乙酸水溶液(含50 mmol/L磷酸盐,pH 6.8)提取,HLB固相萃取柱净化,采用HILIC-MS/MS对目标物进行定性和定量分析。采用SIELC Obelisc R色谱柱,以0.5%(体积分数)甲酸水溶液和乙腈为流动相进行梯度洗脱分离,在正离子模式下检测,外标法定量。该方法在2.5~100 mug/L范围内线性关系良好(r>0.99),检出限(LOD)为2.0 mug/kg,定量限(LOQ)为5.0 mug/kg。在空白蜂蜜样品中进行5.0、20.0、100.0 mug/kg 3个水平的加标回收试验,方法的平均回收率为86.9%~113.2%,精密度在10%以下。该方法简单、快速、灵敏,重复性好,可用于蜂蜜中链霉素和双氢链霉素的定量测定。
英文摘要	An analytical method was developed for the determination of streptomycin and dihydrostreptomycin in honey using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The streptomycin and dihydrostreptomycin residues in the test samples were extracted with 20 g/L trichloroacetic acid aqueous solution (including 50 mmol/L phosphate, pH 6.8) and cleaned on an Oasis HLB solid phase extraction column. The products were separated on a SIELC Obelisc R column with gradient elution using 0.5% (v/v) formic acid aqueous solution and acetonitrile as mobile phases. Streptomycin and dihydrostreptomycin were detected by liquid chromatography-tandem mass spectrometry in the positive ion mode using the external standard method. Under the optimal conditions, streptomycin and dihydrostreptomycin showed good linearity (r>0.99) in the range of 2.5-100 mug/L. The LOD and LOQ of the method was 2.0 mug/kg and 5.0 mug/kg, respectively. The spiked recoveries of the analytes from blank honey samples at the three levels of 5.0, 20.0 and 100.0 mug/kg were in the range of 86.9%-113.2% with the relative standard deviations less than 10%. With the advantages of convenience, rapidity, sensitivity and good repeatability, the method is suitable for the detection of streptomycin and dihydrostreptomycin in honey.
中文关键词	亲水作用色谱-串联质谱 ; 固相萃取 ; 链霉素 ; 双氢链霉素 ; 蜂蜜
英文关键词	hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) solid phase extraction (SPE) streptomycin (STR) dihydrostreptomycin (DHSTR) honey
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6529736
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239376
作者单位	山东省食品药品检验研究院, 济南, 山东 250101, 中国
推荐引用方式 GB/T 7714	魏莉莉,薛霞,刘艳明,等. 亲水作用色谱-串联质谱法测定蜂蜜中链霉素和双氢链霉素残留[J],2019,37(7):735-741.
APA	魏莉莉.,薛霞.,刘艳明.,孙立臻.,程志.,...&赵寅.(2019).亲水作用色谱-串联质谱法测定蜂蜜中链霉素和双氢链霉素残留.色谱,37(7),735-741.
MLA	魏莉莉,et al."亲水作用色谱-串联质谱法测定蜂蜜中链霉素和双氢链霉素残留".色谱 37.7(2019):735-741.