干旱区生态环境知识资源中心(Arid): 嗜盐高效降酚菌株Halomonas sp. H17的筛选及降解苯酚特性

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	嗜盐高效降酚菌株Halomonas sp. H17的筛选及降解苯酚特性
其他题名	Screening and phenol-degrading characteristics of a highly efficient phenoldegrading halophilic bacterial strain Halomonas sp. H17
	赵娜娜 1; 许继飞 1; 宋晓雪 2; 田鹏 3; 丁舒心 3; 赵吉 1
来源期刊	环境科学学报
ISSN	0253-2468
出版年	2019
卷号	39 期号:2 页码:318-324
中文摘要	从巴丹吉林沙漠盐湖沉积物中分离获得1株在高盐环境下高效降解苯酚菌H17.分析了H17生理生化特性、16S rDNA基因序列、苯酚降解特性及动力学,结果表明,H17属于盐单胞菌属( Halomonas sp.) ,能在0~ 20%的盐度下有效降解苯酚,每升外加适量的碳源(葡萄糖浓度0.8 g)和复合氮源( KNO3 1 g、NH_4Cl 5 g、酵母提取物0.2 g和胰蛋白胨0.2 g)能够促进H17的生长及降解苯酚能力.在温度为30 ℃、pH 7~ 8、盐度5~ 10%的条件下,H17均能高效降解苯酚,最高降解率可达到88.5%.该菌株降解苯酚动力学符合Halane模型,经拟合其生长参数为mu_(max) = 0.31 h~(-1),K_S = 191.63 mg·L~(-1),K_i = 683.05 mg·L~(-1) .研究显示H17具有在高盐环境下降解和耐受苯酚的能力,同时对环境有较强的适应性,体现出其在高盐含酚废水实际处理中具有良好的应用价值.
英文摘要	A highly efficient phenol-degrading bacterial strain was separated from the sediments of Badain Jaran Desert Salt Lake in the hypersaline condition. The physiological and biochemical characteristics,16S rDNA gene sequences,and phenol-degrading kinetics were analyzed. The results revealed that the bacterial strain H17 belonged to Halomonas sp.,which could efficiently degrade phenol under 0 ~ 20% salinity. Furthermore,adding appropriate carbon source ( 0.8g glucose) and nitrogen sources ( i.e. 1 g KNO3,5 g NH_4Cl,0.2 g yeast extract,and 0.2 g tryptone) could promote the growth of H17 as well as phenol degradation. Under optimal degradation conditions ( 30 ℃,pH 7~ 8,salinity 5%~ 10%) ,the highest degradation rate of phenol by H17 could reach up to 88.5%. The phenol-degrading kinetics of this bacterial strain was characterized using Halane-model,with the growth parameters of mu_(max) = 0.31 h~(-1),K_S = 191.63 mg·L~(-1),K_i = 683.05 mg·L~(-1) according to the over-fitting. This study showed that the bacterial strain H17 could tolerate and degrade phenol in the hyperhaline environment,suggestive of its application value for hyperhaline phenolic wastewater treatments.
中文关键词	嗜盐菌 ; 苯酚 ; 生物降解 ; 动力学
英文关键词	Halomonas sp. Halophilic bacteria Halomonas sp phenol biodegradation kinetics
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENVIRONMENTAL SCIENCES
WOS研究方向	Environmental Sciences & Ecology
CSCD记录号	CSCD:6412763
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239280
作者单位	1.内蒙古大学;;内蒙古大学生态与环境学院, 环境污染控制与废物资源化内蒙古自治区重点实验室;;, 呼和浩特;;呼和浩特, ;; 010021;;010021; 2.上海师范大学天华学院, 上海 201815, 中国; 3.内蒙古大学生态与环境学院, 呼和浩特, 内蒙古 010021, 中国
推荐引用方式 GB/T 7714	赵娜娜,许继飞,宋晓雪,等. 嗜盐高效降酚菌株Halomonas sp. H17的筛选及降解苯酚特性[J],2019,39(2):318-324.
APA	赵娜娜,许继飞,宋晓雪,田鹏,丁舒心,&赵吉.(2019).嗜盐高效降酚菌株Halomonas sp. H17的筛选及降解苯酚特性.环境科学学报,39(2),318-324.
MLA	赵娜娜,et al."嗜盐高效降酚菌株Halomonas sp. H17的筛选及降解苯酚特性".环境科学学报 39.2(2019):318-324.