干旱区生态环境知识资源中心(Arid): 长期施氮对马铃薯田土壤剖面硝态氮积累及细菌群落结构的影响

Arid

	长期施氮对马铃薯田土壤剖面硝态氮积累及细菌群落结构的影响
其他题名	Effect of long-term nitrogen application on bacterial community structure of potato soil in semi-arid area
	张健; 董星晨; 张鹤; 邓德雷; 罗超越; 张春红; 邱慧珍
来源期刊	甘肃农业大学学报
ISSN	1003-4315
出版年	2019
卷号	54 期号:1 页码:30-41
中文摘要	【目的】揭示长期连续施氮对半干旱地区马铃薯田土壤剖面中硝态氮积累及细菌群落结构的影响. 【方法】通过大田试验设置了6个不同的施氮水平:N0(不施氮,对照)、N_(75)(施氮量75kg/hm~2)、N_(150)(施氮量150kg/hm~2)、N_(225)(施氮量225kg/hm~2)、N_(300)(施氮量300kg/hm~2)和N_(375)(施氮量375kg/hm~2),研究连续4年施氮对旱地马铃薯田土壤剖面中硝态氮的积累以及细菌群落结构的影响.【结果】随着施氮量增加,0~100cm土壤剖面内NO_3~--N含量显著增加(P<0.05),尤其0~20cm土壤表层各施氮处理的NO_3~--N含量分别比对照N0增加了55.8%,113.9%,162.8%,365.1%和988.4%.Miseq高通量测序结果显示,连续施氮显著改变了0~20cm表层土壤的细菌群落结构,在门水平上的土壤优势细菌类群中,随着施氮量增加,各施氮处理土壤放线菌门和拟杆菌门的细菌的丰度分别比对照N0处理增加了6.1%~17.4%和16.4%~46.8%,酸杆菌门的丰度比对照降低了7.8%~26.4%;在纲水平上的土壤优势细菌类群中,与对照N0相比,各施氮处理拟杆菌纲细菌的丰度增加了33.8%~173.3%,AcidobacterGp4和Anaerolineae的相对丰度分别降低16.3%~33.8%、8.1%~22.7%;在属水平上的土壤优势细菌类群中,各施氮处理的Ohtaekwangia和Streptomyces细菌的丰度分别比对照增加了33.6% ~173.1%和3.4%~66.4%,而Gaiella和Solirubrobacter细菌的丰度分别比对照N0降低了6.9%~30.8%和4.5%~27.6%.连续4a过量施氮也显著降低了土壤细菌的群落多样性.RDA冗余分析及相关性分析结果表明,土壤NO_3~--N含量与放线菌门、拟杆菌门、拟杆菌纲、Ohtaekwangia、Streptomyces(链霉菌属)和Lysobacter(溶杆菌属)的细菌的丰度显著正相关,与酸杆菌门、Gaiella、Solirubrobacter(土壤红杆菌属)、Gemmatimonas(芽单胞菌属)的细菌的丰度显著负相关.【结论】连续4a施氮,随着施氮量增加,显著地提高了土壤剖面中硝态氮的含量,与对照N0相比,0~20cm表层土壤细菌丰度随着施氮量增加显著增加,细菌群落结构发生改变,且过量施氮降低了土壤细菌的多样性.RDA分析及person相关性分析表明,长期施氮引起土壤硝态氮含量的变化是造成土壤细菌群落结构发生改变的主效土壤环境因子.
英文摘要	【Objective】In order to announce NO_3~--N accumulation in soil profile of potato field in dryland and the effect of bacterial community structure under continuous nitrogen(N)fertilize application in semiarid areas.【Method】We set up six different levels of N-fertilizer application in field trials:N0(No N-fertilizer,CK),N_(75) (N-fertilizer,75 kg/hm~2),N_(150) (N-fertilizer,150 kg/hm~2),N_(225) (N-fertilizer, 225kg/hm~2),N_(300)(N-fertilizer,300kg/hm~2),N_(375)(N-fertilizer,375kg/hm~2),in order to study NO_3~--N accumulation in soil profile of potato field in dryland and the effect of bacterial community structure by(N) fertilize application for four consecutive years.【Result】With the amount of nitrogen applied,the nitrate (NO_3~--N)content in soil profile of 0~100cm was significantly increased.In particular,the content of NO_3~--N increased by 55.8%,113.9%,162.8%,365.1%and 988.4%in surface layer of 0~20cm compared with N0treatment.Miseq high throughput sequencing results showed that the structure of bacterial community in the surface soil of 0~20cm changed under the condition of continuous nitrogen application. With the amount of nitrogen applied,the abundance of soil Actinomycetes and Bacteroides increased by 6.1%~17.4%and 16.4%~46.8%compared with N0treatment.The abundance of Acidobacter phylum was 7.8%~26.4%lower than that of control.In the dominant class of bacteria,with the increase of nitrogen application,the abundance of Bacteroidetes incret increased by 33.8%~173.3%,and the relative abundance of Acidobacter Gp4and Anaerolineae decreased by 16.3%~33.8%and 8.1%~22.7%.In soil bacteria at dominant genus level,the abundance of Ohtaekwangiaand Streptomyces bacteria increased 33.6% ~73.1%and 3.4%~66.4%,respectively,and the abundance of Gaiellaand Solirubrobacter bacteria decreased 6.9%~30.8%and 4.5%~27.6%respectively,compared with the control N0.The community diversity of soil bacteria was also significantly reduced by excessive application of nitrogen for 4years.RDA redundancy analysis and person correlation analysis showed that the content of NO_3~--N in soil was positively correlated with the abundance of bacteria of Actinomyces,Bacteroidetes,Bacteroidetesincertaesedis, Ohtaekwangia,Streptomyces and Lysobacter.There was a significant negative correlation between the abundance of Gaiella-Solirubrobacter and Gemmatimonas.【Conclusion】The NO_3~--N content in soil profile is significantly increased with the increase of nitrogen application for four consecutive years.Compared with the control N0treatment,the bacterial abundance of 0~20cm surface soil is increased significantly with the increase of nitrogen application rate.The structure of the bacterial community is changed,and excessive application of nitrogen reduced the diversity of soil bacteria.RDA redundancy analysis and person correlation analysis showed that the change of nitrate content in soil caused by nitrogen application for a long time is the main environmental factor of soil bacterial community structure change.
中文关键词	NO_3~--N含量 ; 细菌Alpha多样性 ; 细菌群落结构 ; 相关性分析
英文关键词	NO_3~--N content bacterial alpha diversity bacterial community structure correlation analysis
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Agriculture
WOS研究方向	Agriculture
CSCD记录号	CSCD:6457922
来源机构	甘肃农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239076
作者单位	张健, 甘肃农业大学资源与环境学院, 甘肃省干旱生境作物学重点实验室, 兰州, 甘肃 730070, 中国.; 董星晨, 甘肃农业大学资源与环境学院, 甘肃省干旱生境作物学重点实验室, 兰州, 甘肃 730070, 中国.; 张鹤, 甘肃农业大学资源与环境学院, 甘肃省干旱生境作物学重点实验室, 兰州, 甘肃 730070, 中国.; 邓德雷, 甘肃农业大学资源与环境学院, 甘肃省干旱生境作物学重点实验室, 兰州, 甘肃 730070, 中国.; 罗超越, 甘肃农业大学资源与环境学院, 甘肃省干旱生境作物学重点实验室, 兰州, 甘肃 730070, 中国.; 张春红, 甘肃农业大学资源与环境学院, 甘肃省干旱生境作物学重点实验室, 兰州, 甘肃 730070, 中国.; 邱慧珍, 甘肃农业大学资源与环境学院, 甘肃省干旱生境作物学重点实验室, 兰州, 甘肃 730070, 中国.
推荐引用方式 GB/T 7714	张健,董星晨,张鹤,等. 长期施氮对马铃薯田土壤剖面硝态氮积累及细菌群落结构的影响[J]. 甘肃农业大学,2019,54(1):30-41.
APA	张健.,董星晨.,张鹤.,邓德雷.,罗超越.,...&邱慧珍.(2019).长期施氮对马铃薯田土壤剖面硝态氮积累及细菌群落结构的影响.甘肃农业大学学报,54(1),30-41.
MLA	张健,et al."长期施氮对马铃薯田土壤剖面硝态氮积累及细菌群落结构的影响".甘肃农业大学学报 54.1(2019):30-41.