干旱区生态环境知识资源中心(Arid): 一种沙地苔藓的孢子萌发及愈伤组织诱导

Arid

	一种沙地苔藓的孢子萌发及愈伤组织诱导
其他题名	Spore Germination and Callus Induction of One Bryophyte Collected from Desertification Grassland
	陈振华 1; 陈仕勇 1; 吴昊 1; 田玮琨 1; 李亚萍 2; 周青平 2
来源期刊	分子植物育种
ISSN	1672-416X
出版年	2019
卷号	17 期号:10 页码:3320-3325
中文摘要	本研究以采集自川西北红原县瓦切乡沙化草地的紫色真藓(Bryum purpurascens)成熟孢蒴为材料,研究了其孢子萌发和原丝体发育、不同消毒剂种类、浓度以及不同植物激素处理对其愈伤组织诱导的影响。结果表明:紫色真藓孢蒴最适消毒程序为75%酒精10 s,2% NaClO 20 s (重复2个循环),所获的孢子无菌率为100%,成活率为98.0%。紫色真藓孢子萌发无休眠期,萌发时间为1~2 d,且为多级萌发。以生长20 d原丝体为原材料,采用Knop作为基础培养基,蔗糖浓度为2 g/L,添加0.1 mg/L 6-BA+3 mg/L NAA能够促进愈伤组织的诱导和分化。本研究的结果为紫色真藓快速繁殖体系的建立等提供了科学依据,也为川西北高寒沙化生物结皮等研究提供了参考。
英文摘要	The spore germination, protonema development and the effect of different disinfectants and different concentrations of plant hormone on callus induction were studied, using the mature capsules of Bryum purpurascens collected from the desertification grassland in Waqie, Hongyuan county, northwest Sichuan Province. The results showed that the best disinfectant procedures were 75% alcohol immersion for 10 s and 2% sodium hypochlorite for 20 s with two repeating cycles. The sterile rate of the obtained spores was 100% and the spore survival rate was 98.4%. The dormancy stage was absent during spore development of Bryum purpurascens. The germination time was 1~2 d, and the germination was multistage. Using the protonema grown 20 d as the material, the conditions including the basic Knop medium with 2 g/L sucrose, adding 0.1 mg/L 6-BA+3 mg/L NAA could promote the induction and differentiation of callus. The results of the study would provide the scientific basis for the establishment of rapid propagation system of Bryum purpurascens, as well as the important reference for biological soil crust research of the desertification grassland in Northwest Sichuan Province.
中文关键词	紫色真藓 ; 愈伤组织 ; 孢子萌发,生物结皮
英文关键词	Bryum purpurascens Callus Spore germination Biological soil crusts
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:6502962
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239073
作者单位	1.西南民族大学生命科学与技术学院, 成都, 四川 610041, 中国; 2.西南民族大学,青藏高原研究院, 成都, 四川 610041, 中国
推荐引用方式 GB/T 7714	陈振华,陈仕勇,吴昊,等. 一种沙地苔藓的孢子萌发及愈伤组织诱导[J],2019,17(10):3320-3325.
APA	陈振华,陈仕勇,吴昊,田玮琨,李亚萍,&周青平.(2019).一种沙地苔藓的孢子萌发及愈伤组织诱导.分子植物育种,17(10),3320-3325.
MLA	陈振华,et al."一种沙地苔藓的孢子萌发及愈伤组织诱导".分子植物育种 17.10(2019):3320-3325.