干旱区生态环境知识资源中心(Arid): 转IbLEA14基因杨树增强耐旱性的生理机制

Arid

	转IbLEA14基因杨树增强耐旱性的生理机制
其他题名	Physiological Mechanisms of Transgenic Poplar with Enhancing Tolerance to Drought Stress by Expression IbLEA14 Gene
	乔焕焕 1; 李红兵 2; 孙振玫 3; 可庆波 2; 王仕稳 2; 郭尚洙 4; 邓西平 2
来源期刊	分子植物育种
ISSN	1672-416X
出版年	2019
卷号	17 期号:8 页码:2628-2638
中文摘要	晚期胚胎蛋白富集蛋白(LEA)可作为多功能蛋白参与对多种非生物胁迫的响应。前期研究表明,过量表达甘薯IbLEA14基因能够增强转基因甘薯愈伤组织的耐渗透压和盐胁迫能力。为研究转IbLEA14基因杨树增强耐旱性的生理机制,本试验选取转IbLEA14基因杨树与非转基因杨树(NT)为材料,采用盆栽控水法,进行土壤干旱胁迫与复水处理,测定其形态与生理生化指标。结果表明:(1)干旱胁迫下,转IbLEA14基因杨树与NT株高增长、总生物量积累均受到抑制,但转基因杨树生长状况优于NT;与NT相比,转IbLEA14基因杨树叶片净光合速率较高,丙二醛(MDA)和过氧化氢(H_2O_2)含量较低,较高的脯氨酸(Pro)含量与较低的渗透势保障了转基因杨树较强的保水能力以及较高的叶片相对含水量。(2)复水后,较干旱条件下而言,转IbLEA14基因杨树叶片相对含水量增加,净光合速率升高,MDA和H_2O_2含量降低,Pro含量下降,渗透势升高。研究表明:转IbLEA14基因杨树可减轻干旱胁迫对植株细胞膜及光和系统的伤害,复水后能够更好地修复细胞膜系统与光合系统,加快恢复速度。因此,转IbLEA14基因杨树的耐旱性与旱后复水的恢复能力更好,更具有抗旱性优势。该研究结果将为杨树耐旱育种及应用提供理论依据,未来有望在改善土壤沙漠化方面发挥重要作用。
英文摘要	As a multifunctional protein, late embryogenesis abundant protein (LEA) participates in response to response multiple abiotic stresses. Previous studies have shown that overexpression of IbLEA14 gene in sweet potato could enhance the osmotic potential and salt stress tolerance in callus of transgenic sweet potato.In order tostudy how to improved the physiological mechanism of drought tolerance in IbLEA14 transgenic poplar, this study selected the transgenic poplar lines expressed IbLEA14 gene as material, used pot culture method and measured its morphological and physiological biochemical indexes under drought stress and rewatering. The results indicated: (1) Under drought stress, growth of plant height, total biomass accumulation of transgenic poplar expression IbLEA14 and NT poplar were all suppressed, but the growth condition of transgenic poplar was better than that of NT poplar, compared with NT poplar, the net photosynthesis rate of transgenic poplar was higher, while, MDA and H_2O_2 content was lower, higher Pro content and lower osmotic potential to ensure the higher level of relative water content and water retention capacity of transgenic poplar. (2) Rewatering after drought stress, compared with the situation of drought stress, relative water content on the leaves of transgenic poplar were increased significantly,the net photosynthesis rate of transgenic poplar was gradually increased, the content of MDA and H_2O_2 were sustain decreased, the content of proline was also reduced, and the osmotic potential was increased. The study showed that the transgenic poplar line expression IbLEA14 gene could reduce the damage of plant cell membrane and photosynthetic system under drought stress, when rewatering after drought stress, which could better to repair the cell membrane and photosynthetic apparatus, as well as accelerate the recovery. Therefore, transgenic poplar lines might have better drought tolerance and recovery ability after drought, and have more drought resistance advantages. The results of this study would provide a theoretical basis for drought resistance breeding and application of poplar drought tolerance, which would be expected to play an important role in improving soil desertification in the future.
中文关键词	转基因杨树 ; 干旱胁迫,旱后复水 ; 抗氧化特性 ; 光合速率
英文关键词	Transgenic poplar Drought stress Rewatering Antioxidant properties Photosynthesis rate
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FORESTRY
WOS研究方向	Forestry
CSCD记录号	CSCD:6482522
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239072
作者单位	1.西北农林科技大学生命科学学院;;西北农林科技大学,水土保持研究所, ;;黄土高原土壤侵蚀与旱地农业国家重点实验室, 杨陵;;杨陵, ;; 712100;;712100; 2.西北农林科技大学,水土保持研究所, 黄土高原土壤侵蚀与旱地农业国家重点实验室, 杨陵, 712100; 3.西北农林科技大学林学院, 杨陵, 712100; 4.韩国生命工学研究院, 大田, 305-806, 韩国
推荐引用方式 GB/T 7714	乔焕焕,李红兵,孙振玫,等. 转IbLEA14基因杨树增强耐旱性的生理机制[J]. 西北农林科技大学,2019,17(8):2628-2638.
APA	乔焕焕.,李红兵.,孙振玫.,可庆波.,王仕稳.,...&邓西平.(2019).转IbLEA14基因杨树增强耐旱性的生理机制.分子植物育种,17(8),2628-2638.
MLA	乔焕焕,et al."转IbLEA14基因杨树增强耐旱性的生理机制".分子植物育种 17.8(2019):2628-2638.