干旱区生态环境知识资源中心(Arid): 超高效液相色谱- 串联质谱法同时测定牛奶中38种抗生素残留

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	超高效液相色谱- 串联质谱法同时测定牛奶中38种抗生素残留
其他题名	Simultaneous Determination of 38 Antibiotics Residues in Milk by Ultra-high Performance Liquid Chromatography - Tandem Mass Spectrometry
	方灵; 韦航; 黄彪; 刘文静; 苏德森; 吴妙鸿; 傅建炜
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2019
卷号	38 期号:6 页码:681-686,692
中文摘要	建立了超高效液相色谱-串联质谱(UPLC - MS/MS)同时测定牛奶中5类(磺胺类、喹诺酮类、四环素类、氯霉素类和大环内酯类) 38种抗生素的分析方法。牛奶样品以0. 5 %(体积分数)甲酸乙腈提取,利用Oasis PRiME HLB固相萃取小柱进行净化,经Acquity UPLC HSS T3柱(100 mm * 2. 1 mm i. d. ,1. 8 mum)分离,采用电喷雾离子源,多反应监测模式(MRM)进行定性定量分析。结果表明,38种抗生素在各自线性范围内呈良好的线性关系,相关系数(r~2)均大于0. 99,定量下限为0. 10 ~ 1. 0 mug/kg;在1. 0、5. 0、10. 0 mug/kg加标水平下,平均回收率为70. 7%~ 94. 9%,相对标准偏差(RSD,n = 6)为4. 0%~ 9. 4%。该方法操作简单、灵敏度高、重现性好,适用于牛奶中多种抗生素残留的监测要求。
英文摘要	An analytical method was developed for the simultaneous determination of 38 antibiotics residues in 5 classes of drugs,including sulfonamides,quinolones,tetracyclines,chloramphenicols and macrolides in milk by ultra-high performance liquid chromatography - tandem mass spectrometry (UPLC - MS/MS) . The samples were extracted with acetonitrile containing 0. 5%(by volume) formic acid,and cleaned up with an Oasis PRiME HLB solid phase extraction column. The target components were separated using an Acquity UPLC HSS T3 column(100 mm * 2. 1 mm i. d. ,1. 8 mum) , then identified and quantified by UPLC - MS/MS with electrospray ion source(ESI) under multi-reaction ion monitoring(MRM) mode. Results showed that 38 antibiotics had good linear relationships in their respective linear ranges with correlation coefficients(r~2) higher than 0. 99. The limits of quantitation for 38 antibiotics were between 0. 10 mug/kg and 1. 0 mug/kg. Average recoveries for 38 antibiotics in milk at spiked levels of 1. 0,5. 0,10. 0 mug/kg ranged from 70. 7% to 94. 9%,with relative standard deviations(RSD,n = 6) of 4. 0% - 9. 4%. With the advantages of easy operation,high sensitivity and good reproducibility,the method was applicable for the determination of antibiotics residues in milk.
中文关键词	牛奶 ; 抗生素 ; 磺胺类 ; 喹诺酮类 ; 四环素类 ; 氯霉素类 ; 大环内酯类 ; 超高效液相色谱- 串联质谱(UPLC - MS/MS)
英文关键词	milk antibiotics sulfonamides quinolones tetracyclines chloramphenicols macrolides ultra-high performance liquid chromatography - tandem mass spectrometry(UPLC - MS/MS)
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6514811
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239064
作者单位	福建省农业科学院农业质量标准与检测技术研究所, 福州, 福建 350003, 中国
推荐引用方式 GB/T 7714	方灵,韦航,黄彪,等. 超高效液相色谱- 串联质谱法同时测定牛奶中38种抗生素残留[J],2019,38(6):681-686,692.
APA	方灵.,韦航.,黄彪.,刘文静.,苏德森.,...&傅建炜.(2019).超高效液相色谱- 串联质谱法同时测定牛奶中38种抗生素残留.分析测试学报,38(6),681-686,692.
MLA	方灵,et al."超高效液相色谱- 串联质谱法同时测定牛奶中38种抗生素残留".分析测试学报 38.6(2019):681-686,692.