干旱区生态环境知识资源中心(Arid): 固相萃取/超高效液相色谱-串联质谱法分析乙烯利在棉籽、棉叶和土壤中的残留

Arid

	固相萃取/超高效液相色谱-串联质谱法分析乙烯利在棉籽、棉叶和土壤中的残留
其他题名	Determination of Ethephon Residues in Cotton Seed,Cotton Leaf and Soil by Solid Phase Extraction/Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry
	汤涛; 张昌朋; 吴珉; 许振岚; 何红梅; 张春荣; 赵学平
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2019
卷号	38 期号:1 页码:69-74
中文摘要	建立固相萃取/超高效液相色谱-串联质谱(SPE/UPLC-MS/MS)检测棉籽、棉叶和土壤中乙烯利残留的分析方法。各基质经甲醇-1%甲酸提取,Oasis MAX色谱柱净化,Cortecs Hilic超高效液相色谱柱进行分离,电喷雾负离子多重反应监测(MRM)模式进行测定。结果表明:在0.02 ~1.94 mg/L范围内,乙烯利在溶剂和各基质提取液中的峰面积与其对应的质量浓度间线性关系均良好,r~2≥0.9963;方法的检出限为0.01 mg/kg,定量下限为0.02 mg/kg;在0.02、0.194、3.88 mg/kg加标水平下,乙烯利在3种基质中的回收率为78.3%~ 95.1%,相对标准偏差(RSD)为1.9%~5.2%。该方法具有操作简单、重复性好、灵敏度高等特点,适用于棉籽、棉叶和土壤中乙烯利残留的检测。
英文摘要	A simple and sensitive method was developed for the determination of ethephon residues in cotton seed,cotton leaf and soil by solid phase extraction/ultrahigh performance liquid chromatography-tandem mass spectrometry (SPE/UPLC-MS/MS).The samples were extracted with methanol-0.1% formic acid,purified with an Oasis MAX column,and separated on a Cortecs Hilic column,finally analyzed in multiple reaction monitoring (MRM) mode via negative electrospray ionization(ESI-).Under the optimum condition,the calibration curves for the analyte were linear in the range of 0.02-1.94 mg/L,with correlation coefficients(r~2) not less than 0.996 3.The limits of detection and quantitation for ethephon were 0.01 and 0.02 mg/kg,respectively.At spiked levels of 0.02,0.194 and 3.88 mg/kg,the recoveries for ethephon in three matrices were in the range of 78.3%-95.1% with relative standard deviations(RSDs) of 1.9%-5.2%.With the advantages of simplicity,high sensitivity,and good repeatability,the method could satisfy the requirements for determination of ethephon in cotton seed,cotton leaf and soil.
中文关键词	乙烯利 ; 超高效液相色谱-串联质谱(UPLC-MS/MS) ; 残留 ; 棉籽 ; 棉叶 ; 土壤
英文关键词	ethephon ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) residues cotton seed cotton leaf soil
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6427640
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/239063
作者单位	浙江省农业科学院农产品质量标准研究所, 浙江省部共建国家重点实验室培育基地浙江省植物有害生物防控重点实验室;;农业部农药残留检测重点实验室, 杭州, 浙江 310021, 中国
推荐引用方式 GB/T 7714	汤涛,张昌朋,吴珉,等. 固相萃取/超高效液相色谱-串联质谱法分析乙烯利在棉籽、棉叶和土壤中的残留[J],2019,38(1):69-74.
APA	汤涛.,张昌朋.,吴珉.,许振岚.,何红梅.,...&赵学平.(2019).固相萃取/超高效液相色谱-串联质谱法分析乙烯利在棉籽、棉叶和土壤中的残留.分析测试学报,38(1),69-74.
MLA	汤涛,et al."固相萃取/超高效液相色谱-串联质谱法分析乙烯利在棉籽、棉叶和土壤中的残留".分析测试学报 38.1(2019):69-74.