干旱区生态环境知识资源中心(Arid): 荒漠肉苁蓉花及木质化茎的化学成分定性分析

Arid

	荒漠肉苁蓉花及木质化茎的化学成分定性分析
其他题名	Chemical characterization for flowers and lignified stems of Cistanche deserticola
	刘文静 1; 曹妍 1; 宋青青 1; 郑健 2; 赵云芳 1; 屠鹏飞 1; 李军 1; 宋月林 1
来源期刊	中国中药杂志
ISSN	1001-5302
出版年	2018
卷号	43 期号:18 页码:3708-3714
中文摘要	荒漠肉苁蓉为著名补益类中药肉苁蓉的2种基原植物之一,为寄生植物。一般在其出土前挖取带鳞片的肉质茎作为药用,而出土、开花后的木质化茎和花一般被丢弃。为了探讨这些非传统药用部位的药用价值,该文利用高效液相色谱-离子阱-飞行时间质谱技术(HPLC-IT-TOF-MS)对荒漠肉苁蓉花及木质化茎的化学成分进行深入的定性分析。样品粉末经50%甲醇-水超声提取后,采用Capcell core ADME色谱柱,乙腈-0.1%甲酸水系统梯度洗脱;正/负离子自动多级质谱模式采集高分辨质谱数据;利用对照品推导主要化学类型的质谱裂解规律并指认部分信号,结合对比文献数据以及解析高分辨质谱数据,共对发现的62个信号中的39个进行了结构注释,主要成分包括苯乙醇苷类、环烯醚萜苷类、木质素苷类、糖类等。荒漠肉苁蓉花及木质化茎与肉质茎的化学成分轮廓差异较大,不能等同使用,但其含有较为丰富的木质素和环烯醚萜苷类成分,具有一定的开发利用价值。
英文摘要	As a holoparasitic plant,Cistanche deserticola is one of the two original sources of Cistanches Herba that is one of the most famous tonic medicines,in Chinese Pharmacopoeia. The succulent stems are used for medicinal usage,whereas those lignified stems as well as flowers of less pharmacological importance are usually deserted,suggesting extensive resource waste. Herein,chemical characterization of the flowers along with lignified stems was conducted using HPLC-IT-TOF-MS aiming to explore the medicinal valu of those non-medicinal parts. Following ultrasonication-assisted extraction with 50% aqueous methanol,either flower or lignified stem extract was subjected onto LC-IT-TOF-MS equipped with a Capcell core ADME column to acquire both MS1 and MS2 spectra,and gradient elution was carried out with combinatory 0.1% aqueous formic acid and acetonitrile. Both positive and negative ionization polarities were deployed,resulting in the observation of 62 components,in total. Thirty-nine signals were structurally annotated,including phenylethanoid glycosides,iridoids,lignans and saccharides according to matching with authentic components and literature information, as well as applying the proposed mass fragmentation rules. A total of 62 ones were putatively identified. Above all,lignified stems and flowers should not the qualified substitutes for the succulent stems attributing to the significant differences between the medicinal portion and those parts with less medicinal values.
中文关键词	荒漠肉苁蓉 ; 花和木质化茎 ; 苯乙醇苷类 ; 质谱裂解规律
英文关键词	Cistanche deserticola flowers and lignified stems phenylethanoid glycosides mass fragmentation patterns
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:6360726
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238875
作者单位	1.北京中医药大学中药学院中药现代研究中心, 北京 100029, 中国; 2.中国食品药品检定研究院, 北京 100050, 中国
推荐引用方式 GB/T 7714	刘文静,曹妍,宋青青,等. 荒漠肉苁蓉花及木质化茎的化学成分定性分析[J],2018,43(18):3708-3714.
APA	刘文静.,曹妍.,宋青青.,郑健.,赵云芳.,...&宋月林.(2018).荒漠肉苁蓉花及木质化茎的化学成分定性分析.中国中药杂志,43(18),3708-3714.
MLA	刘文静,et al."荒漠肉苁蓉花及木质化茎的化学成分定性分析".中国中药杂志 43.18(2018):3708-3714.