干旱区生态环境知识资源中心(Arid): 固相微萃取-HPLC测定生物样品中异丙酚含量

Arid

	固相微萃取-HPLC测定生物样品中异丙酚含量
其他题名	Content Determination of Propofol in Biological Samples by Solid Phase Microextraction-HPLC
	安琪; 李莉; 王婷婷; 刁娟娟
来源期刊	中国现代应用药学
ISSN	1007-7693
出版年	2018
卷号	35 期号:8 页码:1124-1129
中文摘要	目的采用固相微萃取(solid phase microextraction,SPME)-HPLC联用技术测定生物样品中异丙酚的含量。方法采用Oasis HLB固相微萃取小柱对样品进行前处理,Shim-pack VP-ODS色谱柱(250 mm*4.6 mm,5 mum),乙腈-水(1%三氟乙酸调pH值至4.00)(70∶30)为流动相,流速1.0 mL·min~(-1),激发波长276 nm,发射波长301 nm,柱温40 ℃。结果异丙酚和卡马西平分离度良好。异丙酚在1.2~21.0 mg·L~(-1)内线性关系良好(r=0.999 7),平均方法回收率为98%~104%,RSD<5%;日内、日间精密度RSD均<5%。结论该方法简便、专属性好,能有效消除部分内源性物质的干扰,适用于异丙酚的血药浓度监测和临床药动学研究。
英文摘要	OBJECTIVE To establish a method for the content determination of propofol in biological samples by solid phase microextraction(SPME)-HPLC.METHODS Samples were pretreated by Oasis HLB-SPME column and analyzed by HPLC consisting of a Shim-pack VP-ODS colum (250 mm*4.6 mm,5 mum),and using a mixture solution of acetonitrile-water (adjusting the pH to 4.00 with 1% trifluoroacetic acid) (70∶30) as the mobile phase.The flow rate was 1.0 mL·min~(-1),the excitation wavelength was 276 nm,the emission wavelength was 301 nm,and the column temperature was 40 ℃.RESULTS The separation of propofol and carbamazepine was good.The method showed a good linearity in the range of 1.2-21.0 mg·L~(-1)(r=0.999 7).The methodological recoveries were all between 98% and 104%,the RSD was <5%.Intraday and interday precision were <5%.CONCLUSION This method is simple and specific,and can effectively eliminate the interference of certain endogenous substances.It is suitable for the therapeutic drug monitoring of propofol in clinic and pharmacokinetic studies.
中文关键词	异丙酚 ; 固相微萃取 ; 高效液相色谱法 ; 生物样品
英文关键词	propofol solid phase microextraction HPLC biological samples
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:6320047
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238859
作者单位	新疆医科大学药学院, 乌鲁木齐, 新疆 830011, 中国
推荐引用方式 GB/T 7714	安琪,李莉,王婷婷,等. 固相微萃取-HPLC测定生物样品中异丙酚含量[J],2018,35(8):1124-1129.
APA	安琪,李莉,王婷婷,&刁娟娟.(2018).固相微萃取-HPLC测定生物样品中异丙酚含量.中国现代应用药学,35(8),1124-1129.
MLA	安琪,et al."固相微萃取-HPLC测定生物样品中异丙酚含量".中国现代应用药学 35.8(2018):1124-1129.