干旱区生态环境知识资源中心(Arid): 基于UPLC-MS的沙米代谢组学分析

Arid

	基于UPLC-MS的沙米代谢组学分析
其他题名	Analysis of Metabolomics in Agriophyllum squarrosum Based on UPLC-MS
	尹晓月 1; 王维东 2; 钱朝菊 3; 范兴科 1; 燕霞 3; 燕高红 4; 马小飞 3
来源期刊	中国实验方剂学杂志
ISSN	1005-9903
出版年	2018
卷号	24 期号:15 页码:51-56
中文摘要	目的:研究沙米地上部分不同组织的代谢物,分析不同组织的差异标志代谢物,挖掘其有效药用活性成分。方法:采用基于UPLC-MS非靶向代谢组学技术对沙米地上组织的药用活性成分进行分析,利用三重四级杆质谱的多反应监测模式(MRM)进行代谢物定量分析,多元统计方法分析沙米地上不同组织差异代谢物。结果:通过代谢组分析共检测到沙米地上组织中的506种代谢物,其中具有药用活性的成分包括黄酮类、生物碱类、多酚类、维生素、萜类、氨基酸等,大部分活性物质在沙米地上组织中含量明显富集,部分代谢物在叶、茎、穗中含量分布存在差异,穗中氨基酸含量显著,叶、茎中黄酮类、多酚类、生物碱类、维生素等次生代谢物含量显著。结论:沙米具有潜在的抗氧化、抗肿瘤、抗炎、抗菌、降血压、降血脂和保护心脑血管等功能。该研究不仅支持利用沙米地上组织开发新药极具前景,同时还为沙米作为新型中草药资源提供重要数据,推动我国干旱区、半干旱区可持续生态农业和健康产业的发展。
英文摘要	Objective:To evaluate the potential medicine usage,we aimed to verifying its medicinal active ingredients of Agriophyllum squarrosum.Method:We explored medicinal active ingredients in the above-ground tissues of A.squarrosum based on UPLC-MS non-targeted metabonomics analysis.Quantitative analysis of metabolites was carried out using a multi-reaction monitoring model(MRM) of triple quadrupole mass spectrometry.The multivariate statistical analysis was used to analyze the difference metabolites in the above-ground tissues of A.squarrosum.Result:With metabonomics analysis,a total of 506 metabolites were detected in the above-ground tissues of A.squarrosum,including flavonoids,alkaloids,polyphenols,vitamins,terpenes,amino acids,etc.Most of the active ingredients had a significant concentration in the aerial part of A.squarrosum,though some metabolites concentration differed among tissues.The spikes mainly contained amino acids,while the stems and leaves were rich in the secondary metabolites,including flavonoids,polyphenols,alkaloids,vitamins,etc.Conclusion:A.squarrosum has a variety of potential functions,such as anti-oxidation,anti-tumor,anti-inflammatory,antibacterial,lowering blood pressure,protecting cardiovascular and cerebrovascular,etc.This work supports that it is highly promising to develop new drugs using the above-ground tissues of A.squarrosum.Meanwhile,it also provides important data for A.squarrosum as a new Chinese herbal medicine resource which can play an important role in sustainable ecological agriculture and health industry in arid and semi-arid regions.
中文关键词	沙米 ; 代谢组学 ; 不同组织 ; 差异代谢物 ; 药用活性成分 ; 氨基酸 ; 维生素
英文关键词	Agriophyllum squarrosum metabolomics different tissues differential metabolites medicinal active ingredients amino acids vitamins
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:6309052
来源机构	中国科学院西北生态环境资源研究院
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238828
作者单位	1.中国科学院西北生态环境资源研究院;;中国科学院大学, 甘肃省寒区旱区逆境生理与生态重点实验室;;, 兰州;;, ;;北京 730000;;100049; 2.健康元集团深圳市海滨制药有限公司, 深圳, 广东 518000, 中国; 3.中国科学院西北生态环境资源研究院, 甘肃省寒区旱区逆境生理与生态重点实验室, 兰州, 甘肃 730000, 中国; 4.甘肃奇正藏药有限公司, 兰州, 甘肃 730000, 中国
推荐引用方式 GB/T 7714	尹晓月,王维东,钱朝菊,等. 基于UPLC-MS的沙米代谢组学分析[J]. 中国科学院西北生态环境资源研究院,2018,24(15):51-56.
APA	尹晓月.,王维东.,钱朝菊.,范兴科.,燕霞.,...&马小飞.(2018).基于UPLC-MS的沙米代谢组学分析.中国实验方剂学杂志,24(15),51-56.
MLA	尹晓月,et al."基于UPLC-MS的沙米代谢组学分析".中国实验方剂学杂志 24.15(2018):51-56.