干旱区生态环境知识资源中心(Arid): 短期盐胁迫下盐穗木的转录组分析

Arid

	短期盐胁迫下盐穗木的转录组分析
其他题名	Transcriptomic Analysis of the Halostachys caspica in Response to Short-term Salt Stress
	张丽丽; 张富春
来源期刊	植物研究
ISSN	1673-5102
出版年	2018
卷号	38 期号:1 页码:91-99
中文摘要	盐穗木(Halostachys caspica)是荒漠盐碱地广泛分布的盐生植物,具有极强的耐盐性。为揭示盐胁迫下盐穗木基因组层面的基因表达变化特性,通过对300和500mmol·L~(-1) NaCl胁迫3h的盐穗木同化枝进行了转录组测序。有效序列组装共得到153298条平均长度为643bp的unigenes,进行GO和KEGG功能聚类,分别获得47个GO功能小类和118个KEGG通路。差异表达基因分析显示,短期低盐(300mmol·L~(-1))响应基因有4432个,高盐(500mmol·L~(-1))响应基因有2580个,两个胁迫的共差异基因有1245个,主要富集在细胞过程、代谢过程和响应刺激等类别中。从短期盐胁迫下盐穗木转录组筛选出渗透调节和活性氧清除的相关基因,大多为上调基因。说明盐穗木能够通过促进渗透调节和增强活性氧清除提高短期的盐胁迫适应能力。
英文摘要	As a halophyte with strong salt tolerance, Halostachys caspica widely distributes in desert saline-alkali land. In order to reveal the genomic changes of gene expression under salt stress, transcriptome sequencing of H.caspica assimilating branches with treatments of 300 and 500 mmol·L~(-1) NaCl for 3 h was performed. A total of 153 298 unigenes with an average length of 643 bp were obtained with clean reads assembled. The 47 subclasses and 118 KEGG pathways were enriched in the GO terms and KEGG pathways, respectively. Differentially expressed genes analysis showed that there were 4 432 and 2 580 unigenes in response to low salt (300 mmol·L~(-1)) and high salt (500 mmol·L~(-1)) in short-term stress, respectively. The 1 245 unigenes were the common differentially expressed genes in the two salt stresses. They were mainly enriched in cellular process, metabolic process and response to stimulus. The osmotic regulation and reactive oxygen species (ROS) scavenging genes were screened out, and most of them were up-regulated. Therefore, H.caspica could improve short-term salt stress adaptation by enhancing the osmotic adjustment and ROS scavenging.
中文关键词	盐穗木 ; 盐胁迫 ; 转录组 ; 差异基因 ; 渗透调节 ; 活性氧
英文关键词	Halostachys caspica salt stress transcriptome differentially expressed genes osmotic adjustment reactive oxygen species
语种	中文
国家	中国
收录类别	CSCD
WOS类目	HORTICULTURE
WOS研究方向	Agriculture
CSCD记录号	CSCD:6153524
来源机构	新疆大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238640
作者单位	新疆大学生命科学与技术学院;;新疆生物资源基因工程重点实验室, ;;新疆生物资源基因工程重点实验室, 乌鲁木齐;;乌鲁木齐, ;; 830046;;830046
推荐引用方式 GB/T 7714	张丽丽,张富春. 短期盐胁迫下盐穗木的转录组分析[J]. 新疆大学,2018,38(1):91-99.
APA	张丽丽,&张富春.(2018).短期盐胁迫下盐穗木的转录组分析.植物研究,38(1),91-99.
MLA	张丽丽,et al."短期盐胁迫下盐穗木的转录组分析".植物研究 38.1(2018):91-99.