干旱区生态环境知识资源中心(Arid): 库布齐沙地生物土壤结皮中解磷菌的分离鉴定及解磷能力

Arid

	库布齐沙地生物土壤结皮中解磷菌的分离鉴定及解磷能力
其他题名	Identification and isolation of phosphorus-solubilizing bacteria of biological soil crusts in Kubqi sandland and their phosphorus-solubilizing capability
	贾丽娟 1; 唐凯 1; 高晓丹 1; 徐慧欣 1; 李蘅 1; 孟建宇 1; 袁立敏 2; 冯福应 1
来源期刊	应用与环境生物学报
ISSN	1006-687X
出版年	2018
卷号	24 期号:2 页码:390-394
中文摘要	解磷菌对生物土壤结皮(Biological soil crusts,BSCs)中不同形态磷之间的转化起重要的生物驱动作用.以无机磷和有机磷培养基分离筛选库布齐沙地BSCs中的解磷菌,基于16S rRNA基因进行分类鉴定;采用钼锑钪比色法测定菌株解无机磷能力;采用钒钼比色法测定其解植酸磷能力,以植酸酶活性表示.结果共分离得到33株可在无机磷(磷酸钙)培养基上生长迅速的细菌,以Bacillus属和Sphingomonas属为主;其中18株菌可以使PVK培养基变色,解无机磷能力较强,达0.422-3.531 mg/mL;这18株菌中有15株可在植酸钙培养基上生长,其植酸酶活力为2.45-20.84 IU/mL,主要为Sphingomonas属.本研究揭示了库布齐沙地BSCs中可培养解磷菌的类群组成及其解磷能力,结果可为认识和利用荒漠解磷菌提供理论依据和实践基础.
英文摘要	Phosphate-solubilizing bacteria(PSB) play a fundamental biological role in the transformation of different phosphorus forms in biological soil crusts(BSCs). To investigate the community composition of PSB in BSCs of Kubqi sandland and capability to dissolve phosphorus, inorganic and organic phosphorus medium was used to isolate phosphorussolubilizing bacteria. A preliminary identification was based on the 16S rRNA gene; spectrophotometry of molybdateantimony- scandium and vanadium-ammonium-molybdate were used to measure the inorganic and organic phosphorussolubilizing capabilities, respectively; the organic phosphorus-solubilizing ability was indicated by phytase activity. The results showed that a total of 33 phosphorus bacteria were isolated from BSCs in Kubqi sandland, which grew rapidly on inorganic phosphorus medium, and were dominated by Bacillus and Sphingomonas; among them, 18 strains changed the color of PVK medium with inorganic phosphorus solubilizing capability of 0.422-3.531 mg/mL; 15 of the 18 strains grew on phytate-Ca medium with phytase activity of 2.45-20.84 IU/mL, and Sphingomonas was dominant. This study preliminarily revealed the composition and ability of PSB in BSCs of Kubqi sandland, which provides a basis for understanding and using desert PSB for other applications.
中文关键词	生物土壤结皮 ; 解磷菌 ; 磷酸钙 ; 植酸磷
英文关键词	16S rDNA biological soil crusts phosphorus-solubilizing bacteria calcium phosphate phosphorus-phytate 16S rRNA
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6248044
来源机构	内蒙古农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238593
作者单位	1.内蒙古农业大学生命科学学院应用与环境微生物研究所, 呼和浩特, 内蒙古 010018, 中国; 2.内蒙古自治区林业科学研究院, 呼和浩特, 内蒙古 010010, 中国
推荐引用方式 GB/T 7714	贾丽娟,唐凯,高晓丹,等. 库布齐沙地生物土壤结皮中解磷菌的分离鉴定及解磷能力[J]. 内蒙古农业大学,2018,24(2):390-394.
APA	贾丽娟.,唐凯.,高晓丹.,徐慧欣.,李蘅.,...&冯福应.(2018).库布齐沙地生物土壤结皮中解磷菌的分离鉴定及解磷能力.应用与环境生物学报,24(2),390-394.
MLA	贾丽娟,et al."库布齐沙地生物土壤结皮中解磷菌的分离鉴定及解磷能力".应用与环境生物学报 24.2(2018):390-394.