干旱区生态环境知识资源中心(Arid): 基于叶绿体DNA非编码序列的蒙古扁桃谱系地理学研究

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	基于叶绿体DNA非编码序列的蒙古扁桃谱系地理学研究
其他题名	Molecular Phylogeography of Amygdalus mongolica (Rosaceae) Based on Chloroplast DNA Non-coding Sequences
	段义忠 1; 白春梅 2; 段春燕 2; 申烨华 2
来源期刊	西北植物学报
ISSN	1000-4025
出版年	2018
卷号	38 期号:9 页码:1625-1633
中文摘要	该研究选取中国西北干旱区第三纪孑遗植物蒙古扁桃(Amygdalus mongolica),基于叶绿体DNA非编码trnH-psbA序列对蒙古扁桃17个居群324个个体进行了谱系地理学研究。结果表明:(1)蒙古扁桃trnH-psbA序列长度350bp,变异位点63个,共有9种单倍型,居群间总遗传多样性为(Ht)为0.758,居群内平均遗传多样性为(Hs)为0.203,贺兰山东麓及阴山南麓边缘的居群具有较高的单倍型多样性及核苷酸多样性并固定较多特有单倍型,推测这2个地区是蒙古扁桃在第四纪冰期时的重要避难所。(2)AMOVA分析表明,居群间的遗传变异为83.84%,居群内的遗传变异为16.16%,居群间遗传分化系数N_(st)>G_(st)(N_(st)=0.733,G_(st)=0.655,P>0.05),表明蒙古扁桃不存在明显的谱系地理结构;根据单倍型地理分布及网络关系图,把蒙古扁桃自然地理居群分为东、西两大地理组群,而且东、西地理组群没有共享单倍型;居群遗传结构分析表明,两大地理组群遗传分化较大。(3)蒙古扁桃居群在间冰期或冰期后经历了近期的居群扩张,由于奠基者效应使得多数居群只固定了单一的单倍型。
英文摘要	Amygdalus mongolicais a Tertiary relict plants widely distributed on the arid region of Northwest China,non-coding chloroplast DNA was amplified and sequenced in 324 individuals from 17 populations sampled from the entire distribution range of A.mongolica.The main results are as follows:(1)the length of trnH-psbA sequence was 350 bp,a total of 63 polymorphic sites were identified and 9 haplotypes were recovered.Total genetic diversity was 0.758,average genetic diversity within populations was 0.203.Populations from the Helan Mountains and southern foot of Yin Mountain owned high haplotype diversity and nucleotide diversity and fixed more private haplotypes,which suggested that the eastern margin of the Helan Mountains and southern foot of Yin Mountain were important refugium during the Quaternary glaciation.(2)AMOVA analysis suggest that 83.84% of total variation existed among populations and 16.16%existed within population,there is no significant phylogeographic structure(N_(st)=0.733,G_(st)=0.655,P>0.05).The natural populations of A.mongolica could be divided into eastern group and western group based on the geographical distribution and network of haplotypes,there were no shared cp- DNA haplotypes between the two groups.The high level of genetic differentiation and genetic diversity were found based on the population genetic analysis,and no significant phylogeography was existed in the populations of A.mongolica.(3)The populations of A.mongolicahave occurred the expansion events during the interglacial periods or post-glacial.Founder effect occurred during the population expansion which lead to only one haplotype in the most populations.
中文关键词	蒙古扁桃 ; 谱系地理学 ; 遗传分化 ; 叶绿体DNA
英文关键词	Amygdalus mongolica phylogeography genetic differentiation cpDNA
语种	中文
国家	中国
收录类别	CSCD
WOS类目	GENETICS HEREDITY
WOS研究方向	Genetics & Heredity
CSCD记录号	CSCD:6365570
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238495
作者单位	1.榆林学院;;西北大学化学与材料学院, 陕西省陕北矿区生态修复重点实验室;;, 榆林;;西安, 陕西;; 719000;;710069, 中国; 2.西北大学化学与材料学院, 西安, 陕西 710069, 中国
推荐引用方式 GB/T 7714	段义忠,白春梅,段春燕,等. 基于叶绿体DNA非编码序列的蒙古扁桃谱系地理学研究[J],2018,38(9):1625-1633.
APA	段义忠,白春梅,段春燕,&申烨华.(2018).基于叶绿体DNA非编码序列的蒙古扁桃谱系地理学研究.西北植物学报,38(9),1625-1633.
MLA	段义忠,et al."基于叶绿体DNA非编码序列的蒙古扁桃谱系地理学研究".西北植物学报 38.9(2018):1625-1633.