干旱区生态环境知识资源中心(Arid): 不同前处理方法对猪组织中喹诺酮类兽药残留检测效果对比

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	不同前处理方法对猪组织中喹诺酮类兽药残留检测效果对比
其他题名	Comparison of Different Sample Pretreatments for the Analysis of Quinolone Residues in Porcine Tissues
	王杰; 裴斐; 李彭; 白宇杰; 方勇; 杨文建; 马宁; 胡秋辉
来源期刊	食品科学
ISSN	1002-6630
出版年	2018
卷号	39 期号:18 页码:309-314
中文摘要	采用超高效液相色谱-三重四极杆质谱和飞行时间质谱技术,比较EMR-L、Oasis PRiME HLB和液液萃取法对猪组织中16种喹诺酮类兽药残留检测效果的影响。目标兽药以基质匹配工作曲线进行定量,在1.0~100.0 mug/L范围内线性关系良好,在5、10、20 mug/kg加标量条件下使用超高效液相色谱-串联质谱检测, EMR-L和Oasis PRiME HLB方法处理的猪组织中加标回收率(71.7%~106.9%、70.7%~118.3%)、相对标准偏差(0.2%~16.8%、0.1%~17.8%)、定量限(0.13~0.56、0.27~1.36 mug/kg)和检出限(0.04~0.23、0.08~0.41 mug/kg)均满足方法学要求。针对不同基质成分样品,采用不同的前处理技术减少假阳性结果的产生,以适用于日益增长的动物源性食品兽药残留检测的需求。本研究采用的2种方法操作简便、灵敏度高、重复性好,满足对猪组织中多兽药残留的定性、定量分析。
英文摘要	This study investigated the influence of different sample pretreatments: enhanced matrix removal-lipid (EMR-L) for lipid, Oasis PRiME hydrophile lipophilic balance (HLB) and liquid-liquid extraction (LLE) on the assay of 16 quinolone residues in porcine tissues by ultra-high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry (UPLC-TQ-MS/MS) or quadrupole-time-of-flight tandem mass spectrometry (Q-TOF/MS). The target compounds were quantified by the matrix-matched calibration curve. Good linearity was observed for all analytes in the range of 1.0-100.0 mug/L. The recoveries using the EMR-L and Oasis PRiME HLB methods at spiked concentration levels (5, 10 and 20 mug/kg) were 71.7%-106.9% and 70.7%-118.3% with relative standard deviations of 0.2%-16.8% and 0.1%-17.8%, respectively; the limits of quantification of the two methods were 0.13-0.56 and 0.27-1.36 mug/kg and the limits of detection were 0.04-0.23 and 0.08-0.41 mug/kg, respectively. All these figures of merit met the requirements for the determination of quinolones. In order to avoid false positive results, a suitable sample pretreatment method should be chosen for different matrix samples. The two pretreatment methods used in this study, thanks to their ease of operation, high sensitivity and good repeatability, could be suitable for the qualitative and quantitative analysis of multiple veterinary drug residues in porcine tissues.
中文关键词	液液萃取 ; 喹诺酮类 ; 猪组织
英文关键词	EMR-L Oasis PRiME HLB EMR-L Oasis PRiME HLB liquid-liquid extraction quinolones porcine tissues
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6340790
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238241
作者单位	南京财经大学食品科学与工程学院, 江苏省现代粮食流通与安全协同创新中心;;江苏高校粮油质量安全控制及深加工重点实验室, 南京, 江苏 210023, 中国
推荐引用方式 GB/T 7714	王杰,裴斐,李彭,等. 不同前处理方法对猪组织中喹诺酮类兽药残留检测效果对比[J],2018,39(18):309-314.
APA	王杰.,裴斐.,李彭.,白宇杰.,方勇.,...&胡秋辉.(2018).不同前处理方法对猪组织中喹诺酮类兽药残留检测效果对比.食品科学,39(18),309-314.
MLA	王杰,et al."不同前处理方法对猪组织中喹诺酮类兽药残留检测效果对比".食品科学 39.18(2018):309-314.