干旱区生态环境知识资源中心(Arid): 固相萃取柱净化-超高效液相色谱-串联质谱法快速测定液态乳中14种真菌毒素

Arid

	固相萃取柱净化-超高效液相色谱-串联质谱法快速测定液态乳中14种真菌毒素
其他题名	Determination of 14 Mycotoxins in Liquid Milk by Solid-Phase Extraction Coupled with Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry
	孙晓冬; 郝杰; 毛婷; 邵瑞婷; 姜洁
来源期刊	食品科学
ISSN	1002-6630
出版年	2018
卷号	39 期号:18 页码:292-301
中文摘要	建立使用固相萃取柱净化液态乳中14种真菌毒素(黄曲霉毒素B1、黄曲霉毒素B2、黄曲霉毒素G1、黄曲霉毒素G2、黄曲霉毒素M1、赭曲霉毒素A、橘霉素、T-2毒素、杂色曲霉素、伏马毒素B1、伏马毒素B2、玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇、青霉酸)多残留的超高效液相色谱-串联质谱检测方法。样品经含0.1%甲酸-乙腈沉淀蛋白和提取,固相萃取柱Oasis PRiME HLB净化后,以0.1%甲酸溶液与乙腈为流动相,经ACQUITY UPLC HSS T3色谱柱(2.1 mm*100 mm,1.8 mum)分离,梯度洗脱,采用电喷雾-正离子多反应监测模式,外标法定量。结果表明:14种真菌毒素的定量限(RSN≥10)为0.5~5 mug/kg,高、中、低3个添加水平时,平均回收率为67.7%~112.7%,相对标准偏差为0.43%~7.28%。该方法的检测速度快,净化效果较好,基质干扰较少,灵敏度高,结果准确、可靠,可用于液态乳中真菌毒素的检测分析。
英文摘要	A rapid method was established for detecting 14 mycotoxins (aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, aflatoxin M1, ochratoxin A, citrinin, T-2 toxin, sterigmatocystin, fumonisin B1, fumonisin B2, zearalenone, deoxynivalenol, and penicillic acid) in liquid milk by solid-phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were defatted and extracted with 0.1% formic acid acetonitrile and the extract was cleaned up with Oasis PRiME HLB solid-phase extraction column. The analytes were separated on HSS T3 column (2.1 mm * 100 mm, 1.8 mum) with a mobile phase composed of 0.1% formic acid and acetonitrile using gradient elution. Detection was carried out by tandem mass spectrometry using positive ionization in the multiple-reaction monitoring (MRM) mode. An external standard method was applied for quantification. Results showed that the limits of quantitation (LOQs) of 14 mycotoxins in matrices were between 0.5 and 5 mug/kg. Average recoveries at 3 different spiked levels were 67.7%-112.7% with relative standard deviation (RSD) of 0.43%-7.28%. With the advantages of rapidity, high purification efficiency, low matrix interference and accurate and reliable results, the method is useful for the analysis of mycotoxins in liquid milk.
中文关键词	液态乳 ; 固相萃取柱 ; 超高效液相色谱-串联质谱 ; 真菌毒素 ; 快速
英文关键词	liquid milk solid-phase extraction column ultra-performance liquid chromatography-tandem mass spectrometry mycotoxins rapid detection
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:6340788
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238240
作者单位	北京市食品安全监控和风险评估中心, 北京 100094, 中国
推荐引用方式 GB/T 7714	孙晓冬,郝杰,毛婷,等. 固相萃取柱净化-超高效液相色谱-串联质谱法快速测定液态乳中14种真菌毒素[J],2018,39(18):292-301.
APA	孙晓冬,郝杰,毛婷,邵瑞婷,&姜洁.(2018).固相萃取柱净化-超高效液相色谱-串联质谱法快速测定液态乳中14种真菌毒素.食品科学,39(18),292-301.
MLA	孙晓冬,et al."固相萃取柱净化-超高效液相色谱-串联质谱法快速测定液态乳中14种真菌毒素".食品科学 39.18(2018):292-301.