干旱区生态环境知识资源中心(Arid): 戈壁异常球菌Dgl5蛋白生物学功能研究

Arid

	戈壁异常球菌Dgl5蛋白生物学功能研究
其他题名	Biological Identification of Dgl5 in Deinococcus gobiensis I-0
	张亨 1; 刘盈盈 2; 陈云 2; 平淑珍 2; 王劲 1
来源期刊	生物技术通报
ISSN	1002-5464
出版年	2018
卷号	34 期号:3 页码:177-184
中文摘要	LEA5C(Group 5C Late Embryogenesis Abundant)家族蛋白参与非生物胁迫反应并以分子伴侣的形式保护细胞内生物大分子不受损伤。分离于新疆戈壁沙漠的戈壁异常球菌(Deinococcus gobiensis)I-0具有极端的电离辐射、氧化、干燥等抗性。功能基因组注释表明Dgo_CA1605编码蛋白属于LEA5C家族,命名为Dgl5,对Dgl5生理功能展开研究。采用同源重组的方法构建Dgl5重组表达菌株。非生物胁迫实验结果表明,Dgl5能够显著增强表达菌株BL21盐胁迫抗性,最大限度地保护宿主菌免受反复冻融的损伤;体外生理生化实验结果表明在反复冻融条件下,Dgl5能够有效保护苹果酸脱氢酶(MDH)和乳酸脱氢酶(LDH)酶学活性。因此推测,在冷冻、高盐胁迫条件下,Dgl5蛋白通过保护细胞体内相关酶类的活性,增强宿主菌抵抗外界复杂多变的极端环境。
英文摘要	Proteins in LEA5C(Group 5C Late Embryogenesis Abundant)family could involve in the responses to abiotic stresses and protect bio-macromolecules in cells from damages under abiotic stresses, due to their functions as molecular chaperones. The Deinococcus gobiensis I-0 isolated from Xinjiang Gobi Desert possessed highly strong resistance to ionizing radiation,oxidation,and desiccation. The genomic annotation of D. gobiensis I-0 indicated that Dgo_CA1605 encoding protein belonged to LEA5C family,named as Dgl5. Accordingly, the function of Dgl5 was investigated in this article. Recombinant expression strain Dgl5 was constructed by homologous recombination. The abiotic stress results revealed that Dgl5 significantly enhanced high-salt resistance of expression strain BL21 and maximally protected the host from the damages in freeze-thaw cycles. Likewise,the physiological and biochemical experiments in vitro demonstrated that the enzymatic activities of malate dehydrogenase and lactate dehydrogenase were effectively maintained due to Dg15 under freeze-thaw cycles. Therefore,we speculated that the improved resistance of the host adapting to harsh environment such as frozen and high-salt was achieved by Dg15 protecting cellular enzyme activities.
中文关键词	戈壁异常球菌 ; 非生物胁迫 ; 分子伴侣
英文关键词	LEA5C LEA5C Deinococcus gobiensis I-0 abiotic stress molecular chaperone
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MICROBIOLOGY
WOS研究方向	Microbiology
CSCD记录号	CSCD:6215947
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238225
作者单位	1.西南科技大学生命科学与工程学院;;中国农业科学院生物技术研究所, ;;, 绵阳;;, ;;北京 621000;;100081; 2.中国农业科学院生物技术研究所, 北京 100081, 中国
推荐引用方式 GB/T 7714	张亨,刘盈盈,陈云,等. 戈壁异常球菌Dgl5蛋白生物学功能研究[J],2018,34(3):177-184.
APA	张亨,刘盈盈,陈云,平淑珍,&王劲.(2018).戈壁异常球菌Dgl5蛋白生物学功能研究.生物技术通报,34(3),177-184.
MLA	张亨,et al."戈壁异常球菌Dgl5蛋白生物学功能研究".生物技术通报 34.3(2018):177-184.