干旱区生态环境知识资源中心(Arid): 陇东旱作果园生草对土壤细菌群落组成的影响

Arid

	陇东旱作果园生草对土壤细菌群落组成的影响
其他题名	Effects of grass-planting on soil bacterial community composition of apple orchard in Longdong arid region
	钱雅丽; 梁志婷; 曹铨; 杨宪龙; 沈禹颖; 王先之
来源期刊	生态学杂志
ISSN	1000-4890
出版年	2018
卷号	37 期号:10 页码:3010-3017
中文摘要	以陇东旱塬13年生秦冠苹果园为对象,采用高通量测序技术分析鸭茅(Dactylis glomerata) 、白三叶(Trifolium repens)和紫花苜蓿(Medicago sativa)生草模式下0 ~ 10 cm土壤细菌群落及特异菌属组成特征,明晰生草覆盖后土壤细菌群落多样性变化规律,为陇东旱作果园建立最优生草管理方式提供依据。结果表明: 3种生草模式下,土壤细菌群落中相对丰度前3位的菌门为变形菌门(Proteobacteria) (48% ~ 52%) 、拟杆菌门(Bacteroidetes) (14%~19%)和酸杆菌门(Acidobacteria) (10%~17%);与对照相比,鸭茅、白三叶和紫花苜蓿处理土壤细菌beta-变形菌纲(Betaproteobacteria)相对丰度分别增加19% ~ 38%,黄杆菌纲(Flavobacteriia)相对丰度分别增加31%~ 65%,土壤溶杆菌属(Lysobacter)相对丰度分别增加37%~93%,苯基杆菌属(Phenylobacterium)相对丰度分别增加45%~ 52%;不同生草模式下土壤均发现特异菌属,梭菌属(Clostridium)出现在鸭茅模式中,该菌属促进土壤氮素积累;侏囊菌属(Nannocystis)存在于白三叶模式中,该菌属分布在有机质丰富环境中;芽孢杆菌属(Bacillus)主要出现在种植白三叶和紫花苜蓿的土壤中,该菌属与植物固氮有较强关联性;果园生草后土壤细菌多样性有增加趋势,可以促进土壤有益特异菌属产生,从而起到调节土壤微环境的作用。
英文摘要	To examine the impacts of grass-planting on soil bacterial diversity in apple orchard, high-throughput sequencing technology was used to analyze bacterial community and composition of specific bacteria genera in top soil (0-10 cm) in a 13-year-old Qinguan apple orchard under planting different grass species including orchard grass (Dactylis glomerata),white clover (Trifolium repens) and alfalfa (Medicago sativa) in Longdong arid region,Gansu. The results showed that the top three phyla with highest relative abundance were Proteobacteria (48%-52%), Bacteroidetes (14%-19%) and Acidobacteria (10%-17%) under the three grass-planting models. Compared to the control,the relative abundance of Betaproteobacteria increased by 19%-38%,Flavobacteriia by 31%-65%,Lysobacter by 37%-93% and Phenylobacterium by 45%-52% respectively under different grass-planting patterns. Clostridium,which promotes soil nitrogen accumulation,was observed only in the orchard grass treatment. Nannocystis,favoring organically rich environments,existed only in the white clover treatment. Bacillus,which has a strong association with plant nitrogen fixation,mainly occurred in the soil where white clover and alfalfa were cultivated. Our results suggested that soil bacteria diversity increased under grass-planting in the apple orchard. Grass-planting can promote the occurrence of specific beneficial bacteria in the soil and thus play a role in regulating soil microenvironment.
中文关键词	黄土高原 ; 果园生草 ; 微生物 ; 群落特征 ; 特异菌属
英文关键词	Loess Plateau grass-planting in orchard microorganism community characteristic specific bacteria genus
语种	中文
国家	中国
收录类别	CSCD
WOS类目	HORTICULTURE
WOS研究方向	Agriculture
CSCD记录号	CSCD:6345160
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238203
作者单位	兰州大学草地农业科技学院;;草地农业生态系统国家重点实验室;;草业科学国家级实验教学示范中心, ;;草地农业生态系统国家重点实验室;;, 兰州;;兰州;;兰州, ;;;; 730020;;730020;;730020
推荐引用方式 GB/T 7714	钱雅丽,梁志婷,曹铨,等. 陇东旱作果园生草对土壤细菌群落组成的影响[J]. 兰州大学,2018,37(10):3010-3017.
APA	钱雅丽,梁志婷,曹铨,杨宪龙,沈禹颖,&王先之.(2018).陇东旱作果园生草对土壤细菌群落组成的影响.生态学杂志,37(10),3010-3017.
MLA	钱雅丽,et al."陇东旱作果园生草对土壤细菌群落组成的影响".生态学杂志 37.10(2018):3010-3017.