干旱区生态环境知识资源中心(Arid): 藏北退化高寒草原土壤细菌和真菌多样性分析

Arid

	藏北退化高寒草原土壤细菌和真菌多样性分析
其他题名	Analysis on the Diversity of Soil Bacterial and Fungal Communities in the Degraded Alpine Grassland in Northern Tibet Plateau
	荣新山 1; 何敏 1; 王从彦 1; 李彦1 ; 戴志聪 1; 梁止水 2; 万凌云 1; 郜志勇 3; 姜坤 1; 伍丙德 1; 张璐 1; 周嘉伟 1
来源期刊	生态环境学报
ISSN	1674-5906
出版年	2018
卷号	27 期号:9 页码:1646-1651
中文摘要	藏北高寒高原为西藏自治区主要牧区。在人类因素和自然因素双重作用下,目前藏北高寒草原退化程度比较高,严重影响当地社会､经济和环境健康与生态安全的可持续发展。草原退化的重要标志之一是土壤质量退化,特别是土壤微生物多样性明显下降,因此,探究藏北退化高寒草原土壤质量以及细菌和真菌多样性对评价其生态系统结构和功能具有重要作用。运用宏基因组学方法,基于高通量测序技术,对藏北退化高寒草原土壤细菌和真菌群落多样性进行分析。结果显示,藏北退化高寒草原土壤化学成分组成以SiO_2为主,占比为63.5%。土壤颗粒粒径分布介于10~0.1 mm之间,其中介于5~1 mm之间土壤颗粒占比为51%。细菌多样性、丰富度和相对丰度均显著高于真菌。放线菌门、变形菌门和酸杆菌门是藏北退化高寒草原土壤细菌群落的优势门类,占比分别为44.9%、16.4%和14.5%;而土壤真菌群落的优势门类主要为子囊菌门、其他类和担子菌门,占比分别为71.5%、14.3%和8.3%。通过对细菌和真菌多样性、丰富度和相对丰度进行计算,得出真菌/细菌比仅分别为0.725、0.260和0.258,表明藏北高寒草原土壤肥力已比较低下,且生态系统呈退化趋势。
英文摘要	Northern Tibet Plateau is the main pastoral area in Tibet Autonomous Region. At present, alpine grassland in Northern Tibet Plateau has been degraded with high degree under the dual effects both of human and natural factors, which can seriously affect the sustainable development of local social and economic condition as well as the environmental health and ecological security. One of the important signs of desertification in grassland is the degradation of soil quality, especially the reduction of the diversity of soil microorganisms. Thus, it is important to analysis soil quality and the diversity of soil bacterial and fungal communities in order to explore the structure and function of the degraded alpine grassland in Northern Tibet Plateau. The present study aims to analyze the soil quality and the diversity of soil bacterial and fungal communities by using metagenomics based on high-throughput sequencing technology. Result showed that the soil chemical composition of the degraded alpine grassland in Northern Tibet Plateau was mainly SiO_2 accounting for 63.5%. The size distribution of soil particles ranged from 10 to 0.1 mm, among which the proportion of soil particles from 5 to 1 mm was 51%. The diversity, richness and relative abundance of soil bacterial communities were significantly higher than those of soil fungal communities. Actinobacteria, Proteobacteria, and Acidobacteria were the most abundant phyla in soil bacterial communities, according for 44.9%, 16.4% and 14.5%, respectively, while Ascomycota, Other and Basidiomycota were the most abundant phyla in soil fungal communities, according for 71.5%, 14.3% and 8.3%, respectively. The ratio of fungal to bacteria based on the diversity, richness and relative abundance was only 0.725, 0.260 and 0.258, respectively, implying that the soil fertility of the degraded alpine grassland in Northern Tibet Plateau was low and the ecosystem had degraded.
中文关键词	退化 ; 藏北高原 ; 高寒草原 ; 细菌多样性 ; 真菌多样性 ; 高通量测序
英文关键词	degraded Northern Tibet Plateau alpine grassland bacterial diversity fungal diversity high-throughput sequencing
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6332069
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238083
作者单位	1.江苏大学环境与安全工程学院/环境健康与生态安全研究院, 镇江, 江苏 212013, 中国; 2.东南大学土木工程学院, 南京, 江苏 210096, 中国; 3.青海碧城生态环境科技有限公司, 西宁, 青海 810001, 中国
推荐引用方式 GB/T 7714	荣新山,何敏,王从彦,等. 藏北退化高寒草原土壤细菌和真菌多样性分析[J],2018,27(9):1646-1651.
APA	荣新山.,何敏.,王从彦.,李彦.,戴志聪.,...&周嘉伟.(2018).藏北退化高寒草原土壤细菌和真菌多样性分析.生态环境学报,27(9),1646-1651.
MLA	荣新山,et al."藏北退化高寒草原土壤细菌和真菌多样性分析".生态环境学报 27.9(2018):1646-1651.