干旱区生态环境知识资源中心(Arid): 固相萃取-超临界流体色谱-质谱联用同时快速测定中成药和保健食品中的12种抗过敏化学药物

Arid

	固相萃取-超临界流体色谱-质谱联用同时快速测定中成药和保健食品中的12种抗过敏化学药物
其他题名	Simultaneous rapid determination of 12 anti-allergic chemical drugs in Chinese traditional patent medicine and health food by supercritical fluid chromatography tandem mass spectrometry with solid phase extraction
	杨直; 彭彦; 金朦娜; 林丽琴
来源期刊	色谱
ISSN	1000-8713
出版年	2018
卷号	36 期号:9 页码:889-894
中文摘要	建立了固相萃取-超临界流体色谱-质谱联用(SPE-SFC-MS/MS)快速检测中成药和保健食品中12种抗过敏化学药物的分析方法。样品经甲醇超声提取,Oasis MCX固相萃取柱净化。采用Waters Trefoil CEL1色谱柱(150 mm3.0 mm, 2.5 mum),以CO_2为流动相A,甲醇-氨水(100∶0.1, v/v)为流动相B,进行梯度洗脱。流速为1.2 mL/min,柱温和背压分别为45 ℃和12.410~6 Pa。12种抗过敏化学药物以电喷雾离子源在正离子或负离子模式下用多反应监测(MRM)方式进行监测,整个分析过程在10 min内完成。结果表明,12种化学药物在5~250 mug/L范围内线性关系良好,相关系数(r)均≥0.998,检出限(LOD)为0.141~0.262 mug/L,定量限(LOQ)为0.703~1.308 mug/L。3种加标水平(10、20和100 mug/L)下,12种化学药物的平均回收率为76.1%~112.5%,相对标准偏差(RSD)为1.1%~8.3%。该法简便,灵敏性高,实用性强,可用于抗过敏类中成药和保健食品中非法添加抗过敏化学药物的检测。
英文摘要	An analytical method was developed for simultaneous rapid determination of 12 anti-allergic chemical drugs in Chinese traditional patent medicine and health food by supercritical fluid chromatography tandem mass spectrometry with solid phase extraction (SPE-SFC-MS/MS). Samples were extracted with methanol by sonification and then purified by Oasis mixed-model cation exchange SPE. The extracts were separated on a Waters Trefoil CEL1 (150 mm3.0 mm, 2.5 mum) column with a mobile phase consisting of carbon dioxide-methanol containing 0.1% (v/v) ammonia water in a gradient elution mode, at a flow rate of 1.2 mL/min. The column temperature was 45 ℃ and the back pressure was 12.410~6 Pa. The whole analysis was completed in 10 min. The 12 anti-allergic chemical drugs were detected by an electrospray ion source in positive or negative modes with a multiple reaction monitoring (MRM) mode. The calibration curves of the 12 anti-allergic chemical drugs showed good linearities in the range of 5-250 mug/L with the correlation coefficients (r)≥0.998. The limits of detection (LODs) were 0.141-0.262 mug/L, and the limits of quantification (LOQs) were 0.703-1.308 mug/L. The recoveries of the 12 anti-allergic chemical drugs at spiked levels of 10, 20 and 100 mug/L were in the range of 76.1%-112.5%, and the relative standard deviations (RSDs) were 1.1%-8.3% (n=6). The method is simple, sensitive and reliable. It has been successfully used for the detection of illegally added anti-allergic chemical drugs in Chinese traditional patent medicine and health food.
中文关键词	超临界流体色谱 ; 质谱 ; 固相萃取 ; 抗过敏化学药物 ; 中成药 ; 保健食品
英文关键词	supercritical fluid chromatography (SFC) mass spectrometry (MS) solid phase extraction (SPE) anti-allergic chemical drug Chinese traditional patent medicine health food
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6328841
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238048
作者单位	杭州市食品药品检验研究院, 杭州, 浙江 310022, 中国
推荐引用方式 GB/T 7714	杨直,彭彦,金朦娜,等. 固相萃取-超临界流体色谱-质谱联用同时快速测定中成药和保健食品中的12种抗过敏化学药物[J],2018,36(9):889-894.
APA	杨直,彭彦,金朦娜,&林丽琴.(2018).固相萃取-超临界流体色谱-质谱联用同时快速测定中成药和保健食品中的12种抗过敏化学药物.色谱,36(9),889-894.
MLA	杨直,et al."固相萃取-超临界流体色谱-质谱联用同时快速测定中成药和保健食品中的12种抗过敏化学药物".色谱 36.9(2018):889-894.