干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法同时检测动物组织中15种保水功能药物

Arid

	超高效液相色谱-串联质谱法同时检测动物组织中15种保水功能药物
其他题名	Simultaneous determination of 15 water-holding functional drugs in animal tissues using ultra performance liquid chromatography-tandem mass spectrometry
	刘洪斌 1; 姚喜梅 2; 田亚平 1; 张鹭 1; 蔡英华 1; 姜艳彬 1; 李颖 1
来源期刊	色谱
ISSN	1000-8713
出版年	2018
卷号	36 期号:4 页码:400-407
中文摘要	建立了超高效液相色谱-串联质谱检测动物组织中15种保水功能药物残留的分析方法。样品经含1.0% (v / v)甲酸的乙腈溶液提取和Oasis PRiME HLB SPE柱净化后,采用Acquity UPLC BEH C18色谱柱(50 mm*2.1 mm,1.7 mum)分离,以甲醇和0.1% (v / v)甲酸水溶液为流动相进行梯度洗脱,在电喷雾电离(ESI)源、正离子模式下,采用MRM监测模式进行数据采集。在1.0~ 50.0 mug / kg范围内,15种保水功能药物的线性关系良好,相关系数均≥0.994 9;定量限均低于1.0 mug / kg。通过添加回收试验表明,动物组织中15种保水功能药物的平均回收率为60.0% ~ 111.0%,批内RSD为0.56% ~ 11.5%,批间RSD为2.31% ~ 14.8%。该方法满足动物组织中该药物多残留的检测要求,为应对动物源性食品中筛查风险隐患和监控非法添加提供了新思路。
英文摘要	A method based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS)was developed for the determination of 15 water-holding functional drugs in animal tissues. The analytes were extracted with acetonitrile containing 1.0% (v / v) methanol,purified by Oasis PRiME HLB SPE column,and analyzed by Acquity UPLC BEH C18 column (50 mm*2.1 mm,1.7 mum)using methanol and 0.1% (v / v)formic acid aqueous solution as the mobile phases. The analytes were detected using an electrospray ionization (ESI) source under the MRM mode. The calibration curves of the analytes were linear in the range of 1.0-50.0 mug / kg (r≥0.994 9),and the limits of quantification were all less than 1.0 mug / kg in animal tissues. The recoveries of the 15 water-holding functional drugs ranged from 60.0% - 111.0%in animal tissue samples,with the intra and inter RSDs of 0.56% -11.5% and 2.31% - 14.8%,respectively. The method can meet the requirements for the determination of the drug residues in animal tissues. It provides a new idea to identify potential hazards in animal-derived foods and to monitor illegal addition.
中文关键词	超高效液相色谱-串联质谱 ; 残留 ; 保水功能药物 ; 动物组织
英文关键词	ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) residues water-holding functional drugs animal tissues
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6239147
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238046
作者单位	1.中国动物疫病预防控制中心, 北京 102609, 中国; 2.中国标准化协会, 北京 100048, 中国
推荐引用方式 GB/T 7714	刘洪斌,姚喜梅,田亚平,等. 超高效液相色谱-串联质谱法同时检测动物组织中15种保水功能药物[J],2018,36(4):400-407.
APA	刘洪斌.,姚喜梅.,田亚平.,张鹭.,蔡英华.,...&李颖.(2018).超高效液相色谱-串联质谱法同时检测动物组织中15种保水功能药物.色谱,36(4),400-407.
MLA	刘洪斌,et al."超高效液相色谱-串联质谱法同时检测动物组织中15种保水功能药物".色谱 36.4(2018):400-407.