干旱区生态环境知识资源中心(Arid): 盐胁迫下双峰驼结肠组织环境适应能力研究

Arid

	盐胁迫下双峰驼结肠组织环境适应能力研究
其他题名	Study on Environmental Adaptability of Bactrian Camel (Camelidae bactrianus) Colon Tissue Under Salt Stress
	凌宇; 齐昱; 孟凡华; 曹俊伟; 王申元; 周欢敏; 张焱如
来源期刊	农业生物技术学报
ISSN	1674-7968
出版年	2018
卷号	26 期号:10 页码:1723-1736
中文摘要	我国北方中西部畜牧地区的水源多为高含盐量水源,普通家畜不能适应这样的水源,从而影响了北方地区畜牧业的发展。双峰驼(Camelidae bactrianus)作为适应北方高盐水源的物种,其盐适应机制尚不清楚。本研究针对双峰驼盐适应能力,对大量进食食盐与正常条件下双峰驼的结肠组织进行转录组测序及数据分析,从转录组学角度了解大量进盐环境下骆驼结肠组织对盐环境的适应机制。结果显示,进盐组测得reads 52 882 246条,对照组测得51 533 346条;进盐组reads总比对率为86.41%,唯一比对率为84.61%;对照组总比对率为92.48%,唯一比对率为91.62%;进盐组与对照组共获得差异表达基因2 450个,其中上调表达基因1 136个,下调表达基因1 314个。基因本体(Gene Ontology, GO)分析显示,进盐组下调表达基因显著富集的term共17条,上调表达基因显著富集的term共28条。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)富集分析显示,下调表达基因显著富集到的包括剪接体、RNA转运、基础转录因子、真核生物中核糖体的生物合成、RNA降解、氧化磷酸化和蛋白输出功能通路。上述结果表明,在盐胁迫条件下,结肠组织下调表达的基因多于上调表达基因,具有统计意义的生物功能分析显示,下调表达基因多参与RNA过程和蛋白质合成通路。这些基因有利于骆驼在高盐环境下减少RNA的合成,降低结肠组织的代谢速率。本研究对区域特色畜牧品种的盐适应机制进行解析,为高含盐水源畜牧地区育种、提高区域资源利用率提供分子理论依据。
英文摘要	The water sources in the livestock areas in the central and western parts of northern China are mostly high-salt water sources. Ordinary livestock cannot adapt to such water sources, thus affecting the development of animal husbandry in the northern regions. Bactrian camel (Camelidae bactrianus) has a species capable of adapting to high salt water source in the extreme environment of northern desert, but its adaptation mechanism is still unclear. In this study, bactrian camel’s salt adaptability was concerned and bactrian camel colon tissue under salt stress conditions and normal conditions was sequenced to understand the mechanism of adaptability of camel’s colon tissues to a salt stress environment. The results showed that 52 882 246 reads were measured in the test group and 51 533 346 in the control group; the total mapped rate of reads in the test group was 86.41%, the uniquely mapped rate was 84.61%, the total mapped rate of reads in the control group was 92.48%, the uniquely mapped rate was 91.62 %; 2 450 differentially expressed genes were obtained, among which 1 136 genes were up-regulated and 1 314 were down-regulated. Gene Ontology (GO) analysis results showed that there were 17 terms that had significantly down-regulated expression of genes and 28 terms that had significantly up-regulated expression of genes. There was no statistically significant enrichment term in the up-regulated expression of genes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the down-regulated expression genes were significantly enriched to include spliceosome, RNA transport, basal transcription factors, ribosome biogenesis in eukaryotes, RNA degradation, oxidative phosphorylation and protein export; up-regulation of expression of genes were not found in enriched pathways. The above results indicated that genes with down-regulated expression in colon were more than upregulated expressed genes in salt stress. Statistically significant biological function analysis showed that downregulated expression genes were involved in RNA processes and protein synthesis pathways. These genes were favorable for reducing RNA synthesis and reducing the metabolic rate of colon tissue. This study analyzes the salt adaptation mechanism of regional characteristic livestock breeds, and provides salt adaptation molecular theory for breeding, thereby improving regional resource utilization.
中文关键词	双峰驼 ; 结肠 ; 转录组 ; 盐代谢
英文关键词	Bactrian camel Colon Transcriptome Salt metabolism
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6357993
来源机构	内蒙古农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/238023
作者单位	内蒙古农业大学生命科学学院, 呼和浩特, 内蒙古 010019, 中国
推荐引用方式 GB/T 7714	凌宇,齐昱,孟凡华,等. 盐胁迫下双峰驼结肠组织环境适应能力研究[J]. 内蒙古农业大学,2018,26(10):1723-1736.
APA	凌宇.,齐昱.,孟凡华.,曹俊伟.,王申元.,...&张焱如.(2018).盐胁迫下双峰驼结肠组织环境适应能力研究.农业生物技术学报,26(10),1723-1736.
MLA	凌宇,et al."盐胁迫下双峰驼结肠组织环境适应能力研究".农业生物技术学报 26.10(2018):1723-1736.