干旱区生态环境知识资源中心(Arid): 三叶木通叶片解剖结构和光合特征对干旱胁迫的响应

Arid

	三叶木通叶片解剖结构和光合特征对干旱胁迫的响应
其他题名	Responses of Akebia trifoliate leaf anatomical structure and photosynthetic characteristic to drought stress
	吴正花 1; 喻理飞 1; 严令斌 1; 周晨 2; 蔡国俊 3; 张建利 3
来源期刊	南方农业学报
ISSN	2095-1191
出版年	2018
卷号	49 期号:6 页码:1156-1163
中文摘要	【目的】分析干旱胁迫下三叶木通叶片的解剖结构和光合特性,为西南喀斯特石漠化区生态修复植物材料选择提供参考依据。【方法】盆栽喀斯特地区适生植物三叶木通并进行连续干旱胁迫处理,采用常规石蜡切片法和Li-6400光合仪分别测定其幼苗叶片的解剖结构参数和光合参数,分析解剖结构和光合生理特征变化,以及二者间的相关性。【结果】随土壤相对水含量(SRWC)的降低,三叶木通幼苗叶片呈厚度变薄、水含量降低和栅栏组织缩短变紧密等变化趋势;总叶绿素(Chlt)含量、净光合速率(P_n)、气孔导度(G_s)和蒸腾速率(T_r)下降,水分利用效率(WUE)提高;SRWC降至29%时,大部分解剖指标和光合指标呈显著性差异变化(P<0.05)。主成分分析结果表明,大于1.000的叶片解剖指标特征值中有4个主成分的载荷值差异极大,贡献率达88.195%,主要指标为栅栏组织、下表皮、木质部和海绵组织;大于1.000的叶片光合指标特征值有2个主成分的载荷值差异极大,贡献率达83.277%,指标为G_s和Chlt。相关性分析结果表明,SRWC与叶片水含量(LWC)、Chlt、P_n、G_s、胞间CO_2浓度(C_i)、T_r、气孔限制值(L_s)及叶片解剖结构中的叶片厚度、栅栏组织厚度和木质部厚度均呈极显著正相关(P<0.01,下同),与WUE和叶片组织结构疏松度呈极显著负相关。【结论】三叶木通幼苗在干旱胁迫下通过改变叶片结构特征、降低光合作用和提高水分利用效率等方式适应干旱频发的喀斯特环境;SRWC为29%可视为三叶木通幼苗受干旱胁迫的起点;栅栏组织、海绵组织、木质部及Gs可作为三叶木通耐旱性品种筛选的参考指标。
英文摘要	【Objective】The anatomical structure and photosynthetic characteristics of leaves of Akebia trifoliate were observed and measured under drought stress to provide references for selecting suitable plants for ecological restoration in karst rocky desertification area in southwest China.【Method】A. trifoliate,a karst adaptable specie,was taken as material. Through pot experiment and continuous drought stress,seeding leaf anatomical structures were observed by conventional paraffin section,and changes of photosynthetic physiological characteristics were analyzed by measuring photosynthetic parameters with Li-6400. The changes of anatomical structures and photosynthetic characters and the correlation between the two were analyzed.【Result】With soil relative water content(SRWC)decreasing,the thickness of seeding leaf thinned, water content decreased and palisade tissues shortened in the structural features of the leaves of A. trifoliate. Chlorophyll content(Chlt),photosynthetic rate(P_n),stomatal conductance(G_s),transpiration rate(T_r)decreased,water use efficiency (WUE)increased. Most of the anatomical and photosynthetic indicators showed significant difference when SRWC decreased to 29%(P<0.05). The results of principal component analysis showed that the load values of four principal components in the characteristic values of leaf anatomy greater than 1.000 varied greatly,with a contribution rate of 88.195%. The main indicators were palisade tissue,lower epidermis,xylem,and spongy tissue. Among these photosynthetic index eigenvalues of leaves which were larger than 1.000,Gs and Chlt were two main components with large differences in load values,with a contribution rate of 83.277%. The correlation analysis showed that there was significant positive correlation between SRWC and leaf water content(LWC),Chlt,P_n,G_s,C_i,T_r,L_s,leaf thickness,palisade tissue thickness and xylem thickness in leaf anatomy(P<0.01,the same below). There was extremely significant negative correlation between WUE and leaf structure loose degree(SR).【Conclusion】By changing the structure characteristics,reducing photosynthesis and increasing water use efficiency under drought stress,seedlings of A. trifoliate can adapt to the karst environment in southwest China where drought take place frequently. SRWC of 29% can be regarded as the starting point of drought stress of A. trifoliate seedlings. Palisade tissue,spongy tissue,xylem and G_s can be used as reference indexes for the screening of drought-tolerant varieties of A. trifoliate.
中文关键词	三叶木通 ; 干旱胁迫 ; 解剖结构 ; 光合特征 ; 喀斯特
英文关键词	Akebia trifoliate drought stress anatomical structure photosynthetic characteristics karst
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRONOMY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6289731
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237934
作者单位	1.贵州大学生命科学学院;;山地植物资源保护与种质创新教育部重点实验室;;山地生态与农业生物工程协同创新中心, ;;山地植物资源保护与种质创新教育部重点实验室;;山地生态与农业生物工程协同创新中心, 贵阳;;贵阳, ;; 550025;;550025; 2.江西省林业科学院, 南昌, 江西 330000, 中国; 3.贵州省山地资源研究所, 贵阳, 贵州 550001, 中国
推荐引用方式 GB/T 7714	吴正花,喻理飞,严令斌,等. 三叶木通叶片解剖结构和光合特征对干旱胁迫的响应[J],2018,49(6):1156-1163.
APA	吴正花,喻理飞,严令斌,周晨,蔡国俊,&张建利.(2018).三叶木通叶片解剖结构和光合特征对干旱胁迫的响应.南方农业学报,49(6),1156-1163.
MLA	吴正花,et al."三叶木通叶片解剖结构和光合特征对干旱胁迫的响应".南方农业学报 49.6(2018):1156-1163.