干旱区生态环境知识资源中心(Arid): 抗绕实蝇沙棘无性系的筛选及相关化学物质和酶活性分析

Arid

	抗绕实蝇沙棘无性系的筛选及相关化学物质和酶活性分析
其他题名	Screening of Sea-Buckthorn (Hippophae rhamnoides) Clones Resisting Fruit Fly (Rhagoletis batava obseuriosa) (Diptera: Tephritidae) and Analysis of the Related Biochemicals and Enzyme Activities
	刘建凤 1; 董礼龙 2; 曹丹丹 1; 罗红梅 2; 魏建荣 1
来源期刊	林业科学
ISSN	1001-7488
出版年	2018
卷号	54 期号:9 页码:104-113
中文摘要	【目的】筛选抗绕实蝇的沙棘无性系植株,分析不同抗、感植株间相关生化物质种类和含量的差异,为进一步研究抗性机制提供理论基础。【方法】首先通过林间调查和室内生测试验,筛选抗性植株,然后以抗、感沙棘无性系为材料,利用气相色谱-质谱联用仪(GC-MC)和高效液相色谱(HPLC)等,在成虫羽化前和产卵盛期检测抗、感无性系植株相关化学物质含量和酶活性。【结果】1)通过林间调查果实受害率及开展沙棘绕实蝇产卵选择性试验,筛选出抗沙棘绕实蝇的沙棘无性系。2)在产卵盛期,抗虫无性系果实和叶片中总酚含量、可溶性蛋白质和脯氨酸含量均显著高于敏感无性系,在果实中分别高出101. 34%、50. 21%和20. 12%,叶片中分别高出121. 22%、 30. 43%和40. 68%。3)抗、感无性系果实的挥发性物质种类及含量在2种无性系植株间具有显著差异,其中,抗虫无性系的酯类物质种类及含量显著低于敏感无性系,而醇类物质含量明显高于敏感无性系。4)抗虫无性系果实和叶片中非挥发性物质山奈酚含量明显高于敏感无性系,分别高出29. 40%和40. 80%;果实中异鼠李素含量较敏感无性系高出43. 20%,而在叶片中的含量较敏感无性系低41. 34%。5)产卵盛期,抗虫无性系果实中过氧化物酶(POD)、几丁质酶(Chi)、苯丙氨酸解氨酶(PAL)活性较敏感无性系分别高出98. 21%、25. 03%和20. 10%,在叶片中POD和PAL则分别高出33. 40%和36. 30%。【结论】抗、感无性系果实、叶片中化学物质及酶活性均存在较大差异,由此推测,沙棘体内抗虫相关化学物质和酶活性与其抗沙棘绕实蝇的能力密切相关。结果初步揭示沙棘抗虫无性系的生理生化特征,为进一步研究沙棘抗虫性机制及开展抗虫性育种提供依据。
英文摘要	【Objective】Sea-buckthorn (Hippophae rhamnoides) is an important economic and ecological plant in the arid and semi-arid region of China,and Rhagoletis batava obseuriosa (Diptera: Tephritidae) (RBO) is a serious quarantine insect pest that damaged the plant fruit. In order to find the RBO-resistant clones,a field investigation and indoor test were carried out. The difference of the biochemical substances between the resistant-and sensitive-plants were investigated to provide the theoretical basis for further study of physiological and biochemical resistance mechanisms of seabuckthorn to RBO. 【Method】First,the difference of RBO adult’s oviposition biology and fruit damage were compared between RBO-resistant clones and RBO-sensitive clones both in the field and in laboratory. Then,two stages: before the adult emergence and at the peak time of adult oviposition,were chosen to detect biochemical substances and enzyme activities in the fruits and leaves of the resistant- and sensitive-clones using gas chromatography-mass spectrometry (GCMC) and high performance liquid chromatography (HPLC). 【Result】1) Sea-buckthorn clones resisting RBO were obtained by screening the fruit damage in the field and comparing the RBO ovposition rates indoors. 2) At the peak time of adult oviposition,the total phenolics,soluble protein and proline content in RBO-resistant clone were higher than those in RBO-sensitive clones,with increasing of 101. 34%,50. 21% and 20. 12% in the fruits,and 121. 22%,30. 43% and 40. 68% in the leaves,respectively. 3) The volatile compounds were significantly different in the fruits of the two clones. The types and contents of the esters in the RBO-resistant clones were significantly lower than those in the RBO-sensitive clones,while the content of alcohols was significantly higher than that in the RBO-sensitive clones. 4) The content of nonvolatile kaempferol in RBO-resistant clones was significantly higher than that in the RBO-sensitive clones,with increase of 29. 40% and 40. 80% in fruits and leaves,respectively. The content of isorhamnetin in fruits of RBO-resistant was 43. 20% higher than that in RBO-sensitive clones,while the content in the leaves was 41. 34% lower than that in the RBOsensitive clones. 5) At the peak time of adult oviposition,activity of the peroxidase (POD),chitinase and phenylalanine ammonialyase (PAL) in the fruits were 98. 21%,25. 03%,and 20. 10% higher,respectively,and the POD and PAL activities in the leaves of RBO resistant clones were 33. 40% and 36. 30% higher,respectively than those in RBOsensitive clones. 【Conclusion】There were significant differences in the secondary metabolites and enzyme activities in the sea-buckthorn fruits and leaves between the RBO-resistant clones and the RBO-sensitive clones. The results preliminarily revealed the physiological and biochemical characteristics of the RBO-resistance,and provided the basis for further study on the mechanism of the RBO-resistance and the new RBO-resistant clone breeding.
中文关键词	沙棘 ; 沙棘绕实蝇 ; 抗虫性 ; 生化物质 ; 酶活性
英文关键词	sea-buckthorn Rhagoletis batava obseuriosa insect-resistant biochemicals enzyme activity
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FORESTRY
WOS研究方向	Forestry
CSCD记录号	CSCD:6334483
来源机构	中国林业科学研究院沙漠林业实验中心
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237919
作者单位	1.河北大学生命科学学院, 保定, 河北 071002, 中国; 2.中国林业科学研究院沙漠林业实验中心, 磴口, 内蒙古 015200, 中国
推荐引用方式 GB/T 7714	刘建凤,董礼龙,曹丹丹,等. 抗绕实蝇沙棘无性系的筛选及相关化学物质和酶活性分析[J]. 中国林业科学研究院沙漠林业实验中心,2018,54(9):104-113.
APA	刘建凤,董礼龙,曹丹丹,罗红梅,&魏建荣.(2018).抗绕实蝇沙棘无性系的筛选及相关化学物质和酶活性分析.林业科学,54(9),104-113.
MLA	刘建凤,et al."抗绕实蝇沙棘无性系的筛选及相关化学物质和酶活性分析".林业科学 54.9(2018):104-113.