干旱区生态环境知识资源中心(Arid): 气相色谱-电子捕获检测器快速测定水产品中多种农药及兽药残留

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	气相色谱-电子捕获检测器快速测定水产品中多种农药及兽药残留
其他题名	Rapid Determination of Pesticide and Veterinary Drug Residues in Aquatic Products by Gas Chromatography-Electro Capture Detector
	符靖雯; 黄子敬; 陈孟君; 臧国栋; 杨钦沾
来源期刊	理化检验. B, 化学分册
ISSN	1001-4020
出版年	2018
卷号	54 期号:9 页码:1015-1019
中文摘要	称取2.000g样品,加入含0.2%(phi)甲酸的乙腈(85+15)溶液10mL,涡旋混合30s,振荡提取30min,以10 000r·min~(-1)转速离心5min,取上清液。用2mL的含0.2%(phi)甲酸的乙腈(85+15)溶液活化Oasis PRiME HLB小柱后,将上述上清液全部通过Oasis PRiME HLB小柱并收集滤液,于50℃水浴下氮吹至干,加入100muL衍生化试剂[N,O-双(三甲基硅烷基)三氟乙酰胺-三甲基氯硅烷(质量比为99∶1)],涡旋10s,于65℃恒温反应30min后,于50℃水浴下氮吹至干,用正己烷定容至1.0mL,以DB-5/DB-35色谱柱(30m*0.25mm,0.25mum)分离11种拟除虫菊酯类农药和3种氯霉素类兽药,采用电子捕获检测器。11种农药的线性范围为0.10~ 0.80mg·L~(-1),3种兽药的线性范围为5.0~40mg·L~(-1),检出限(3S/N)在0.000 1~0.008 0mug·g~(-1)之间。加标回收率为85.6%~119%,测定值的相对标准偏差(n=6)不大于9.0%。
英文摘要	10mL of acetonitrile(85+15)solution containing 0.2% (phi)formic acid was added to 2.000g of the sample.The mixture was vortexed for 30s,extracted by oscillation for 30min,and centrifuged at 10 000r·min~(-1) for 5min,and the supernatant was taken.After the Oasis PRiME HLB cartridge was activated with 2mL of acetonitrile(85+15)solution containing 0.2% (phi)formic acid,the supernatant was passed through the Oasis PRiME HLB cartridge and the filtrate was collected and dried by N2-blowing in a water bath at 50℃.Then 100muL of derivatization reagent[N,O-bis(trimethylsilyl)trifluoroacetamide-trimethylchlorosilane(mass ration of 99∶1)] was added to the resdiue,and the mixture was vortexed for 10sand let react at a constant temperature of 65℃for 30min.After the mixture was dried by N2-blowing in a water bath at 50 ℃,and the residue was made up to 1.0mL with n-hexane.11pyrethroid pesticides and 3chloramphenicol veterinary drugs were separated on a DB-5/ DB-35column(30m*0.25mm,0.25mum)and determined by using an electron capture detector.The linear range of 11pesticides was 0.10-0.80mg·L~(-1),the linear range of 3veterinary drugs was 5.0-40mg·L~(-1),and the detection limits(3S/N)were between 0.000 1-0.008 0mug·g~(-1).Values of recovery obtained by standard addition method ranged from 85.6%to 119%,and RSDs(n=6)were not more than 9.0%.
中文关键词	气相色谱-电子捕获检测器 ; 净化 ; 农药 ; 兽药 ; 水产品
英文关键词	gas chromatography-electron capture detector purification pesticide veterinary drug aquatic product
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6329081
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237913
作者单位	惠州市农产品质量安全监督检测中心, 惠州, 广东 516008, 中国
推荐引用方式 GB/T 7714	符靖雯,黄子敬,陈孟君,等. 气相色谱-电子捕获检测器快速测定水产品中多种农药及兽药残留[J],2018,54(9):1015-1019.
APA	符靖雯,黄子敬,陈孟君,臧国栋,&杨钦沾.(2018).气相色谱-电子捕获检测器快速测定水产品中多种农药及兽药残留.理化检验. B, 化学分册,54(9),1015-1019.
MLA	符靖雯,et al."气相色谱-电子捕获检测器快速测定水产品中多种农药及兽药残留".理化检验. B, 化学分册 54.9(2018):1015-1019.