干旱区生态环境知识资源中心(Arid): 固相萃取-超高效液相色谱-串联质谱法测定果蔬中34种植物生长调节剂的残留量

Arid

	固相萃取-超高效液相色谱-串联质谱法测定果蔬中34种植物生长调节剂的残留量
其他题名	UPLC-MS/MS Determination of Residual Amounts of 34Plant Growth Regulators in Fruits and Vegetables with Solid Phase Extraction
	陈金斌; 张伊; 张晓景; 王凌杰
来源期刊	理化检验. B, 化学分册
ISSN	1001-4020
出版年	2018
卷号	54 期号:7 页码:774-782
中文摘要	提出了固相萃取-超高效液相色谱-串联质谱法同时测定果蔬中34种植物生长调节剂残留量的方法。样品2.000 0g用乙腈10.0mL提取,经Oasis PRiME HLB固相萃取柱净化。取流出液5.0mL,氮气吹至近干,用甲醇(1+9)溶液溶解残渣并定容至1.0mL。以Waters CSH氟苯基色谱柱为固定相,以甲醇-5mmol·L~(-1)乙酸铵溶液[含0.10%(体积分数)乙酸]为流动相进行梯度洗脱,采用电喷雾正、负离子源和多反应监测模式检测,外标法定量。34种植物生长调节剂在一定范围内呈线性关系,检出限(3S/N)为0.01~0.20mug·kg~(-1)。按标准加入法进行回收试验,回收率为71.0%~115%,测定值的相对标准偏差(n=6)为0.60%~16%。
英文摘要	A method was proposed for the simultaneous determination of residual amounts of 34plant growth regulators in fruits and vegetables by UPLC-MS/MS with solid phase extraction.The sample of 2.000 0g was extracted with 10.0mL of acetonitrile and purified by passing through Oasis PRiME HLB solid phase extraction column.The eluate of 5.0mL was taken and concentrated to dryness by N_2-blowing,and the residue was dissolved and made its volume up to 1.0mL with methanol(1+9)solution.The Waters CSH fluorophenyl chromatographic column was used as stationary phase,and the mixture of methanol and 5mmol·L~(-1) ammonium acetate solution containing 0.1% (phi)acetic acid was used as mobile phase for gradient elution.ESI+,ESI-and the mode of multireaction monitoring were adopted in MS/MS,and external standard method was used for quantification.Linear relationships of 34plant growth regulators were found in definite ranges,with detection limits(3S/N)in the range of 0.01-0.20mug·kg~(-1).Test for recovery was made by standard addition method,giving results in the range of 71.0%-115%,with RSDs(n=6)ranged from 0.60%to 16%.
中文关键词	超高效液相色谱-串联质谱法 ; 植物生长调节剂 ; 固相萃取 ; 果蔬
英文关键词	UPLC-MS/MS plant growth regulator solid phase extraction fruit and vegetable
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6286820
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237911
作者单位	温岭市食品药品检验检测中心, 温岭, 317500
推荐引用方式 GB/T 7714	陈金斌,张伊,张晓景,等. 固相萃取-超高效液相色谱-串联质谱法测定果蔬中34种植物生长调节剂的残留量[J],2018,54(7):774-782.
APA	陈金斌,张伊,张晓景,&王凌杰.(2018).固相萃取-超高效液相色谱-串联质谱法测定果蔬中34种植物生长调节剂的残留量.理化检验. B, 化学分册,54(7),774-782.
MLA	陈金斌,et al."固相萃取-超高效液相色谱-串联质谱法测定果蔬中34种植物生长调节剂的残留量".理化检验. B, 化学分册 54.7(2018):774-782.