干旱区生态环境知识资源中心(Arid): 沙漠干热环境下创伤失血性休克猪模型血清炎症因子的变化特点

Arid

	沙漠干热环境下创伤失血性休克猪模型血清炎症因子的变化特点
其他题名	Characteristics of serum inflammatory factors in porcine model of traumatic hemorrhagic shock in dry-heat environment of desert
	沈才福; 刘江伟; 夏亮; 是文辉; 董翔; 安宗仁; 段越
来源期刊	解放军医学杂志
ISSN	0577-7402
出版年	2018
卷号	43 期号:7 页码:589-593
中文摘要	目的探讨沙漠干热环境下创伤失血性休克(THS)猪血清炎症因子肿瘤坏死因子alpha(TNF-alpha)、白细胞介素-1beta(IL-1beta)、IL-6、IL-10的变化特点。方法健康长白仔猪40只,随机分为常温假手术组(NS组)、常温创伤失血性休克组(NTHS组)、干热假手术组(DS组)、干热创伤失血性休克组(DTHS组),每组10只。DTNS组和DS组在干热环境(温度40.50.5℃,湿度10%2%)下、NTHS组和NS组在常温环境(温度25.00.5℃,湿度35%5%)下暴露3h后建立脾切除和部分肝脏切除的THS模型,NS组和DS组于相应环境暴露后仅行剖腹术后观察。分别于暴露后及休克后0、1、 2、3、8(其中TNF-alpha为6h)、10h抽取颈静脉血,检测各时间点血清TNF-alpha、IL-1beta、IL-6、IL-10水平,比较各组炎症因子的变化特点。结果干热环境暴露后,实验猪血清TNF-alpha、IL-6、IL-10水平均较常温组升高,差异有统计学意义(P<0.05)。THS建模成功后,实验猪血清炎症因子均呈上升趋势,尤以TNF-alpha上升的速度最快、峰值出现最早,DTHS组在1h左右达峰值,且明显高于NTHS组及DS组(P<0.05),而NTHS组在休克后6h才达到峰值;继TNF-alpha之后促炎因子IL-1beta出现峰值时间最早,DTHS组在2h左右即达峰值,且明显高于NTHS组及DS组(P<0.05),NTHS组则在休克后8h才达到峰值;IL-6呈进行性上升趋势,DTHS组自干热环境暴露后即明显高于NTHS组(P<0.05),且一直持续至动物死亡。抗炎因子IL-10水平在DTHS组于1h左右即达峰值,且明显高于NTHS组及DS组(P<0.05),随后逐渐降低,而在NTHS组则一直呈进行性升高,从休克后2h即明显高于NS组(P<0.05)。结论在沙漠干热环境下,THS模型猪血清炎症因子TNF-alpha、IL-1beta、IL-6、IL-10水平升高出现时间早、增长速度快,可能在沙漠干热环境下THS的发生发展过程中具有重要作用。
英文摘要	Objective To investigate the changes of serum inflammatory factors TNF-alpha, IL-1beta, IL-6 and IL-10 in porcine model of traumatic hemorrhagic shock (THS) in dry-heat environment of desert. Methods Forty Landrace piglets were randomly divided into 4 groups (10 each): normal temperature sham operation (NS) group, normal temperature traumatic hemorrhagic shock (NTHS) group, dry-heat sham operation (DS) group and dry-heat traumatic hemorrhagic shock (DTHS) group. The THS model was established after exposure for 3 hours in their own environments, i.e. pigs in DS and DTHS group were exposed to the dry-heat environment (40.50.5℃, humidity: 10%2%), in NS and NTHS group to room temperature (250.5℃, humidity: 35%5%). Pigs in NTHS and DTHS group underwent splenectomy and partial hepatectomy, and in NS group and DS group received only laparotomy and then observation. The jugular blood were extracted 0h, 1h, 2h, 3h, 8h and 10h after exposure (AE), and the serum concentrations of TNF-alpha, IL-1beta, IL-6 and IL-10 were detected, and then the characteristics of serum inflammatory factors of each group were compared and analyzed. Results After exposure (AE), the serum concentrations of TNF-alpha, IL-6 and IL-10 were significantly higher in DS and DTHS group than in NS and NTHS group (P<0.05). After the THS model was successfully established, the serum levels of inflammatory cytokines showed a rising trend, especially the TNF-alpha showed a fastest rising speed with the peak appeared at the earliest. In DTHS group the peak value was reached about 1h AE, and was obviously higher than those in NTHS group and DS group (P<0.05). For IL-1beta, the peak value appeared earlier than those of IL-6 and IL-10, and was significantly higher in DTHS group than in NTHS group and DS group (P<0.05). The inflammatory factor IL-6 showed a progressive upward trend, presented a higher level in DTHS group than in NTHS group (P<0.05), and continued until the death of animals. At the same time, the anti-inflammatory factor IL-10 was released, the peak value appeared at about 1h AE in DTHS group, markedly higher than that in NTHS group and DS group (P<0.05), and then declined gradually; while the IL-10 level showed continuous rising in NTHS group, and was obviously higher than in NS group since about 2h AE (P<0.05). Conclusion In desert dry-heat environment, the serum levels of inflammatory factors TNF-alpha, IL-1beta, IL-6 and IL-10 started rising earlier with a faster growth rate in THS pig, which may play an important role in the process of developing traumatic hemorrhagic shock in the dry-heat environment of desert.
中文关键词	休克,创伤性 ; 休克,出血性 ; 干热环境 ; 沙漠 ; 炎症因子
英文关键词	shock traumatic shock, hemorrhagic dry-heat environment desert inflammatory factors
语种	中文
国家	中国
收录类别	CSCD
WOS类目	SURGERY
WOS研究方向	Surgery
CSCD记录号	CSCD:6297633
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237882
作者单位	新疆军区总医院, 新疆特殊环境医学重点实验室, 乌鲁木齐, 新疆 830000, 中国
推荐引用方式 GB/T 7714	沈才福,刘江伟,夏亮,等. 沙漠干热环境下创伤失血性休克猪模型血清炎症因子的变化特点[J],2018,43(7):589-593.
APA	沈才福.,刘江伟.,夏亮.,是文辉.,董翔.,...&段越.(2018).沙漠干热环境下创伤失血性休克猪模型血清炎症因子的变化特点.解放军医学杂志,43(7),589-593.
MLA	沈才福,et al."沙漠干热环境下创伤失血性休克猪模型血清炎症因子的变化特点".解放军医学杂志 43.7(2018):589-593.