干旱区生态环境知识资源中心(Arid): 荒漠草原植物骆驼蓬根际土壤细菌群落分析

Arid

	荒漠草原植物骆驼蓬根际土壤细菌群落分析
其他题名	Diversity of Bacterial Communities in Rhizosphere Soil of Peganum harmala L. in Desert Steppe
	程琳 1; 陈吉祥 1; 李彦林 1; 王永刚 1; 周永涛 2; 张彦 2
来源期刊	干旱区研究
ISSN	1001-4675
出版年	2018
卷号	35 期号:4 页码:977-983
中文摘要	骆驼蓬( Peganum harmala L. )具有较强耐旱抗寒特性,在维护生态环境中发挥着重要作用。为了解其生态适应及与环境相互作用机制,采用高通量测序和纯培养方法研究了甘肃白银荒漠草原区骆驼蓬根际土壤细菌群落特征,并与周边土壤进行了对比。结果表明:骆驼蓬根际土壤主要优势细菌群为放线菌门( Actinobacteria) 30.01%、变形菌门( Proteobacteria) 23.98%、拟杆菌门( Bacteroidetes) 11.53%、酸杆菌门( Acidobacteria) 10.19%。周边荒漠草原土壤主要优势细菌群为放线菌门55.05%、变形菌门21.11%、酸杆菌门6.07%。可培养细菌类群包括厚壁菌门( Firmicutes) 、变形菌门、放线菌门,其中根际土壤分离的优势菌为短杆菌属( Brevibacterium) 23.53%、芽孢杆菌属( Bacillus) 23.53%、假单胞菌属( Pseudomonas) 17.65%,周边土壤可培养细菌的优势种群为节杆菌属( Arthrobacter) 37.50%、芽孢杆菌属18.75%、假单胞菌属12.50%。骆驼蓬根际微生物群落组成和结构与周边土壤有显著差异,根际土壤微生物数量及多样性指数明显高于非根际土壤。研究结果对了解荒漠植物与根际微生物之间相互作用,揭示土壤微生物促进植物根际土壤物质循环转化作用,筛选促生长有益微生物,为加强荒漠草原生态保护提供科学依据。
英文摘要	Peganum harmala L. is strong in resistance against severe environment, such as drought and low temperature. Plants play an important role in maintaining the local ecological environment. In order to understand the mechanism of ecological adaptation and interaction with environment, the characteristics of bacterial communities in rhizosphere soil of P. harmala in a desert steppe in Baiyin,Gansu Province were studied using the high-throughput sequencing and culture-dependent method,and they were compared with those of surrounding soil. The results showed that the bacterial communities in the rhizosphere soil were consisted of 30.01% Actinobacteria,23.98% Proteobacteria,11.53% Bacteroidetes and 10.19% Acidobacteria. The dominant soil bacterial community in the surrounding desert steppe was composed of 55.05% Actinobacteria,21.11% Proteobacteria and 6.07% Acidobacteria. The cultivable bacterium group included Firmicutes,Proteobacteria and Actinobacteria. In the rhizosphere soil, the dominant bacteria were 23.53% Brevibacterium,23.53% Bacillus,17.65% Pseudomonas and 11.76% Brevundimonas. The dominant populations of bacteria in the surrounding soil were 37.50% Arthrobacter,18.75% Bacillus and 12.50% Pseudomonas. The difference of bacterial communities between the rhizosphere soil of P. harmala and its surrounding desert steppe soil was significant. The bacteria numbers and diversity index in the rhizosphere soil were significantly higher than those of the surrounding desert steppe soil. The results provided the scientific basis for exploring the interaction between the desert steppe plants and their rhizobacteria,and also for understanding the roles of the rhizobacteria in promoting plant growth and soil material transformation.
中文关键词	荒漠草原 ; 骆驼蓬( Peganum harmala L. ) ; 根际土壤 ; 细菌群落 ; 高通量测序 ; 土壤微生物
英文关键词	desert steppe Peganum harmala L. rhizosphere soil bacterial community high throughput sequencing soil microorganism
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6298461
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237670
作者单位	1.兰州理工大学石油化工学院, 兰州, 甘肃 730050, 中国; 2.中石油北京天然气管道有限公司, 北京 100101, 中国
推荐引用方式 GB/T 7714	程琳,陈吉祥,李彦林,等. 荒漠草原植物骆驼蓬根际土壤细菌群落分析[J],2018,35(4):977-983.
APA	程琳,陈吉祥,李彦林,王永刚,周永涛,&张彦.(2018).荒漠草原植物骆驼蓬根际土壤细菌群落分析.干旱区研究,35(4),977-983.
MLA	程琳,et al."荒漠草原植物骆驼蓬根际土壤细菌群落分析".干旱区研究 35.4(2018):977-983.