干旱区生态环境知识资源中心(Arid): 通过式固相萃取净化/UPLC - MS/MS法测定特殊医学用途配方食品中的13种非法添加化学成分

Arid

	通过式固相萃取净化/UPLC - MS/MS法测定特殊医学用途配方食品中的13种非法添加化学成分
其他题名	Determination of 13 Ingredients Illegally Added in Formulated Foods on Special Medical Purposes by Ultra-performance Liquid Chromatography - Tandem Mass Spectrometry with Pass-through Solid Phase Extraction
	励炯; 邱红钰; 贾彦博; 王红青; 李玮
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2018
卷号	37 期号:3 页码:307-312
中文摘要	建立了特殊医学用途配方食品中非法添加的13种降糖类化学成分的通过式固相萃取净化/超高效液相色谱-串联质谱检测方法。样品用90%乙腈水溶液(含1%甲酸)提取后,提取液经Oasis PRiME HLB固相萃取柱净化,以Waters ACQUITY UPLC BEH C_(18)(150 mm * 2.1 mm,1.7 mum)色谱柱分离,以甲醇-含0.1%甲酸的5 mmol /L乙酸铵溶液为流动相梯度洗脱,正离子模式电喷雾电离,配合多反应离子扫描(MRM)定性定量分析目标化合物。考察了提取剂用量、甲酸及乙腈含量对加标回收率的影响,优化了主要的影响因素和实验条件。结果表明: 13种化合物在2 ~ 100 mug /L范围内线性关系良好,相关系数r~2≥0.995 1;方法回收率为80.9%~ 102.8%;定量下限为0.3 ~ 1.0 mug /kg。该方法前处理简单,回收率高,重现性好,可作为降糖类特殊医学用途配方食品中非法添加降糖类化学成分的有效检测方法。
英文摘要	An ultra-performance liquid chromatography - tandem mass spectrometry (UPLC - MS / MS) was developed for the determination of 13 anti - diabetic ingredients illegally added in formulated foods on special medical purposes. Samples were extracted with 90% acetonitrile containing 1% formic acid,and then purified with an Oasis PRiME HLB column. The extraction was on a Waters ACQUITY UPLC BEH C_(18) (150 mm * 2.1 mm,1.7 mum) column with methanol - 5 mmol /L ammonium acetate solution containing 0.1% formic acid as mobile phases by gradient elution. The analysis on the analyte was performed by UPLC - MS /MS under positive electrospray ionization in multiple reaction monitoring(MRM) mode,Effects affecting the recoveries such as dosage of extractant,formic acid and acetonitrile in cleaning-up agent were investigated. The main influence factors and analytical conditions were modified. Results showed that there were good linear relationships for 13 ingredients in the concentration range of 2 -100 mug /L with their correlation coefficients(r~2) no less than 0.995 1. The recoveries at three concentration levels ranged from 80.9% to 102.8% with LOQs of 0.3 - 1.0 mug /kg. With simple pretreatment,high recovery and good reproducibility,the developed method was suitable for the determination of 13 anti - diabetic ingredients illegally added in formulated foods on special medical purposes.
中文关键词	超高效液相色谱- 串联质谱 ; 通过式固相萃取 ; 特殊医学用途配方食品 ; 降糖
英文关键词	ultra-performance liquid chromatography - tandem mass spectrometry (UPLC - MS / MS) dispersive solid phase extraction foods for special medical purpose anti - diabetic
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6200208
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237508
作者单位	杭州市食品药品检验研究院, 杭州, 浙江 310017, 中国
推荐引用方式 GB/T 7714	励炯,邱红钰,贾彦博,等. 通过式固相萃取净化/UPLC - MS/MS法测定特殊医学用途配方食品中的13种非法添加化学成分[J],2018,37(3):307-312.
APA	励炯,邱红钰,贾彦博,王红青,&李玮.(2018).通过式固相萃取净化/UPLC - MS/MS法测定特殊医学用途配方食品中的13种非法添加化学成分.分析测试学报,37(3),307-312.
MLA	励炯,et al."通过式固相萃取净化/UPLC - MS/MS法测定特殊医学用途配方食品中的13种非法添加化学成分".分析测试学报 37.3(2018):307-312.