干旱区生态环境知识资源中心(Arid): 云南省元谋县村民尿液中异菌脲残留影响因素分析

Arid

	云南省元谋县村民尿液中异菌脲残留影响因素分析
其他题名	Detection of urinary iprodione residues among rural residents in Yuanmou county of Yunnan
	关秋艳 1; 方菁 1; 王垠芸 2; 陶汝俊 2
来源期刊	中国预防医学杂志
ISSN	1009-6639
出版年	2017
卷号	18 期号:9 页码:647-650
中文摘要	目的检测云南省元谋县农村居民尿中异菌脲残留浓度,分析异菌脲残留量与居民使用农药时采取防护措施之间的关系。方法抽取云南省元谋县230名农民进行问卷调查及尿样采集,采用超高效液相色谱串联质谱法(UHPLC-MS/MS)测定尿中异菌脲残留量。尿液经Waters Oasis MCX柱固相萃取,用甲醇和5%的氨水甲醇溶液洗脱后离心浓缩,乙腈定容;以0.01%的甲酸乙腈溶液和0.01%的甲酸水溶液为流动相梯度洗脱,经Cosmosil色谱柱(4.6mm*150mm)分离,采用电喷雾离子源正离子模式进行质谱检测。结果230份尿样共检出102份有异菌脲残留,检出率为44.35%,研究发现两个乡镇间居民尿液中异菌脲残留检出率差异有统计学意义(chi~2=6.16,P<0.05);5个村庄间居民尿液中异菌脲残留检出率差异有统计学意义(chi~2=11.49,P<0.05),阳性人群尿液中异菌脲残留量差异有统计学意义(F=3.60, P<0.01);尿液中异菌脲残留未检出人群佩戴口罩、手套、防护服的频率高于检出人群(Z=-4.50、-4.30、-4.94,P<0.01)。结论使用农药时采取防护措施能有效的减少农药与人体的接触,提示居民应合理使用农药,加强自身防护措施,从而减少农药对健康的危害。
英文摘要	Objective To detect iprodione residues in urine samples of rural residents in Yuanmou county of Yunnan,and to analyze the influencing factors. Methods Quota sampling method was used to randomly select 230 rural residents to participate in our questionnaire survey and their urine samples were collected for the detection of iprodione concentration by ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).The urine sample was solid-phase extracted by Waters Oasis MCX and eluted with methanol and methanol solution of 5% aqueous ammonia.After centrifugation and concentration,it was separated on Cosmosil packed column(4.6mm*150mm)with acetonitrile solution of 0.01% formic acid and aqueous solution of 0.01% formic acid as mobile phase by gradient elution.Electrospray ionization source was applied and operated in positive ion mode. Results Iprodione residue was detected in 102 urine samples with the positive rate of 44.35%.The positive rates of iprodione residues were significantly different between rural residents from two local towns(chi~2=6.16,P<0.05)as well as among rural residents from 5 villages(chi~2= 11.49,P<0.05).The concentration of iprodione residues was also different among residents with positive samples(F=3.60,P<0.01).Residents with negative iprodione in urine samples were more likely to wear masks,rubber gloves and protective clothing when exposing to pesticides (Z=-4.50,-4.30,-4.94,P< 0.01). Conclusions Rural residents should properly protect themselves from direct exposure to the pesticides in order to avoid the hazardous effects of pesticides to the body.
中文关键词	异菌脲残留 ; 尿液 ; 防护措施 ; 超高效液相色谱串联质谱 ; 检出率
英文关键词	Iprodione residues Urine sample Protection measures UHPLC-MS/MS Detection rate
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PUBLIC ENVIRONMENTAL OCCUPATIONAL HEALTH
WOS研究方向	Public, Environmental & Occupational Health
CSCD记录号	CSCD:6103610
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237207
作者单位	1.昆明医科大学健康研究所, 昆明, 云南 650500, 中国; 2.昆明医科大学药学院, 云南省天然药物药理重点实验室
推荐引用方式 GB/T 7714	关秋艳,方菁,王垠芸,等. 云南省元谋县村民尿液中异菌脲残留影响因素分析[J],2017,18(9):647-650.
APA	关秋艳,方菁,王垠芸,&陶汝俊.(2017).云南省元谋县村民尿液中异菌脲残留影响因素分析.中国预防医学杂志,18(9),647-650.
MLA	关秋艳,et al."云南省元谋县村民尿液中异菌脲残留影响因素分析".中国预防医学杂志 18.9(2017):647-650.