干旱区生态环境知识资源中心(Arid): 基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质

Arid

	基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质
其他题名	Identification of Effective Substances from Polygonum orientale Flower Extract in H9c2 Myocardial Cells by UHPLC-Q-TOF-MS
	向文英; 梅朝叶; 孙慧园; 覃小丽; 谢玉敏; 巩仔鹏; 郑林; 李月婷; 黄勇
来源期刊	中国实验方剂学杂志
ISSN	1005-9903
出版年	2017
卷号	23 期号:12 页码:90-95
中文摘要	目的: 利用H9c2心肌细胞和UHPLC-Q-TOF-MS技术筛选荭草花抗心肌缺血作用的活性成分。方法: 用心肌细胞选择性地结合荭草花提取液中的活性成分,洗去未结合的其他成分后,使细胞靶点失活,被结合的活性成分从心肌细胞中释放出来,采用Eclipse Plus C_(18)色谱柱(2.1 mm * 100 mm, 1.8 mum),流动相0.1%甲酸水溶液(A) -0.1% 甲酸乙腈溶液(B)梯度洗脱(0 ~ 2 min,95% ~ 79% A; 2 ~ 5 min,79% A; 5 ~ 6.5 min,79% ~ 60% A; 6.5 ~ 7.5 min,60% ~ 55% A; 7.5 ~ 9.5 min,55% ~ 0%A; 9.5 ~ 10 min,0% ~ 95%A),电喷雾离子源(ESI)正、负离子模式下扫描采集数据,通过采用对照品比对及二级碎片离子对化学成分进行鉴定。结果:检测出荭草花提取物中可与心肌细胞结合的17个成分,采用对照品比对后,确定8个色谱峰所表征的化学成分,依次为原儿茶酸,儿茶素,山柰素-3-O-beta-D-葡萄糖苷,槲皮素3-O-alpha-L-鼠李糖苷,山柰素-3-O-alpha-L-鼠李糖苷,N-p-香豆酰酪胺,槲皮素和山柰酚。结论: 通过分析心肌细胞破碎液中与活性细胞相结合的成分,筛选出了荭草花提取物中能与心肌细胞有相互作用的成分,为明确荭草花的药效物质基础提供了科学依据。
英文摘要	Objective: To screen effective substances from Polygonum orientale flower extract by UHPLCQ- TOF-MS technology for H9c2 myocardial cells. Method: The active substances in P. orientale flower extract were combined with H9c2 myocardial cells under imitated physiological environments, then the unattached substances were washed off by phosphate buffer solution (PBS) with pH of 7.4. After that,the conjugated compounds were eluted by PBS with pH of 4.0. These compounds released from target sites were collected and handled through Oasis HLB solid phase extraction column to be condensed. The separation was performed on a Eclipse Plus C_(18) column,mass sepectrometric data were obtained in electrospray ionization (ESI) positive and negative modes. Meanwhile,the chemical components were identified by comparing the retention times with standard substance and secondary fragment ions. Result: Seventeen compounds from P. orientale flower extract were identified preliminarily, and eight compounds were identified as protocatechuic acid, cianidanol,kaempferol, kaempferol-3-O-beta-D-glucoside, quercetin, quercetin-3-O-beta-D-glucoside, kaempferol-3-O-alpha-Lrhamnoside,paprazine. Conclusion: Throngh analysis of the fracture fluid compounds from the H9c2 cells extraction combined with active cells,the bioactive constituents from P. orientale flower extract interacted with myocardial cells are screened,this research provides a scientific fundament for material basis of effectiveness of P. orientale flower.
中文关键词	荭草花提取物 ; 活性成分 ; 山柰素-3-O-beta-D-葡萄糖苷 ; 山柰素-3-O-alpha-L-鼠李糖苷 ; 药效物质基础
英文关键词	UHPLC-Q-TOF-MS UHPLC-Q-TOF-MS Polygonum orientale flower extract effective substances kaempferol- 3-O-beta-D-glucoside kaempferol-3-O-alpha-L-rhamnoside pharmacodynamic basis
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:5982719
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/237177
作者单位	贵州医科大学药学院, 贵州省药物制剂重点实验室;;国家苗药工程技术研究中心, 贵阳, 贵州 550004, 中国
推荐引用方式 GB/T 7714	向文英,梅朝叶,孙慧园,等. 基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质[J],2017,23(12):90-95.
APA	向文英.,梅朝叶.,孙慧园.,覃小丽.,谢玉敏.,...&黄勇.(2017).基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质.中国实验方剂学杂志,23(12),90-95.
MLA	向文英,et al."基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质".中国实验方剂学杂志 23.12(2017):90-95.