干旱区生态环境知识资源中心(Arid): 喜盐鸢尾花色形成关键基因的克隆及表达分析

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	喜盐鸢尾花色形成关键基因的克隆及表达分析
其他题名	Cloning and Expression Analysis of Anthocyanin Biosynthetic Genes from Iris halophila (Iridaceae)
	徐凌云; 王俊丽; 周宜君
来源期刊	植物遗传资源学报
ISSN	1672-1810
出版年	2017
卷号	18 期号:2 页码:340-348
中文摘要	喜盐鸢尾(Iris halophila Pall.)及其变种蓝花喜盐鸢尾(I. halophila Pall. var. sogdiana(Bung)Grubov)因耐盐碱及其多种花色而具有盐碱地园艺开发价值。本文根据喜盐鸢尾内轮花被转录组测序结果,利用基因特异性引物从这2种植物中分别克隆了编码查尔酮合成酶(CHS)、查尔酮异构酶(CHI)、类黄酮-3’,5’-羟基化酶类(F3’5’H-like)等基因的部分片段,并对它们在内轮花被中的表达水平进行实时定量 PCR分析。序列分析结果确认在喜盐鸢尾中所克隆的CHS(311 bp)、CHI(457 bp)、 F3’5’H-like(496 bp)3个基因(部分)未见文献报道与 NCBI等数据库记录。其中F3’5’H-like基因与经典的属于细胞色素P450 CYP75A亚家族的F3’5’H不同,而与万带兰的F3’5’H-like同属于CYP76AB亚家族,为一类新的蓝花相关基因。实时定量 PCR表达分析结果表明,与黄花的喜盐鸢尾相比,蓝花喜盐鸢尾中CHS与 F3’5’H-like显著上调表达,可能是其花色不同于喜盐鸢尾的主要原因。
英文摘要	Iris halophila Pall. and its variety I. halophila Pall. var. sogdiana(Bung) Grubov are potential horticultural plants for arid and saline-alkali land because of their drought and salt tolerant characteristics and various flower color. In order to elucidate the mechanism of different pigmentation in these two species,we cloned 3 partial segments of key genes relating to anthocyanin biosynthesis,named chalcone synthase(CHS) ,chalcone isomerase (CHI) and flavonoid-3’,5’-hydroxylase like (F3’ 5’ H-like) ,from perianth of young flowers of these two Iris plants. Retro-transcription PCR method were employed in cloning and gene-specific primers were designed based on a transcriptome sequencing. The expression of these 3 genes were characterized by a real-time PCR assay. Sequence analysis showed that these 3 genes were first reported in I. halophila Pall. . Among these genes,F3’5’Hlike was different from the classical F3’5’H which belonged to CYP75A subfamily of cytochrome P450 superfamily,it was a homolog of Vanda coerulea Griff. ex Lindl. F3’ 5’ H-like gene which belonged to a new subfamily CYP76AB,a novel type of blue flower related genes. Expression analysis indicated that compared with in the yellow flowered I. halophila Pall. ,CHS and F3’5’ H-like were up-regulated in I. halophila Pall. var. sogdiana (Bung) Grubov and responsible for its blue-violet flowers.
中文关键词	喜盐鸢尾 ; 蓝花喜盐鸢尾 ; 查尔酮合成酶 ; 查尔酮异构酶 ; 类黄酮-3’,5’-羟化酶类
英文关键词	Iris halophila Pall. I. halophila Pall. var. sogdiana(Bung) Grubov chalcone synthase chalcone isomerase flavonoid-3’,5’-hydroxylase like
语种	中文
国家	中国
收录类别	CSCD
WOS类目	HORTICULTURE
WOS研究方向	Agriculture
CSCD记录号	CSCD:5950566
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236980
作者单位	中央民族大学生命与环境科学学院, 北京 100081, 中国
推荐引用方式 GB/T 7714	徐凌云,王俊丽,周宜君. 喜盐鸢尾花色形成关键基因的克隆及表达分析[J],2017,18(2):340-348.
APA	徐凌云,王俊丽,&周宜君.(2017).喜盐鸢尾花色形成关键基因的克隆及表达分析.植物遗传资源学报,18(2),340-348.
MLA	徐凌云,et al."喜盐鸢尾花色形成关键基因的克隆及表达分析".植物遗传资源学报 18.2(2017):340-348.