干旱区生态环境知识资源中心(Arid): 短叶对齿藓可培养内生细菌的分离及其发酵产物对宿主植物的影响

Arid

	短叶对齿藓可培养内生细菌的分离及其发酵产物对宿主植物的影响
其他题名	Isolation of cultivable endophytic bacteria from Didymodon tectorum and effects of extracts from strain fermentation broths on host plant growth
	麻扬; 胡素静; 王潇; 王丽红; 赵东平
来源期刊	应用与环境生物学报
ISSN	1006-687X
出版年	2017
卷号	23 期号:6 页码:1028-1034
中文摘要	采用传统培养法分离内蒙古黄土丘陵区生物结皮的藓类植物优势种短叶对齿藓(Didymodon tectorum)的内生细菌,并观察代表菌株的发酵产物对宿主植物孢子萌发及原丝体生长的影响.共分离出68株内生细菌,基于16S rDNA序列同源性分析及构建的系统发育树显示,它们分属于芽孢杆菌属(Bacillus)下的7个种,其中优势种为枯草芽孢杆菌(B. subtilis).枯草芽孢杆菌菌株DT-1和蜡样芽孢杆菌菌株DT-9的石油醚相、乙酸乙酯相和正丁醇相萃取物在1 mug/mL、10 mug/mL及100 mug/mL三种浓度下对孢子萌发均无显著影响;石油醚相萃取物在3种浓度下不同程度地抑制原丝体生长;而乙酸乙酯相和正丁醇相萃取物则促进原丝体的生长.本研究表明苔藓植物原丝体阶段的生长发育与内生细菌的次生代谢物密切相关,可为干旱、半干旱区藓类生物结皮人工促建提供参考资料和实验依据.
英文摘要	Didymodon tectorum, the dominant species in a community of biological soil crusts in the hilly Loess Plateau region, was selected for investigation. Using traditional culture-dependent methods, we isolated endophytic bacteria from D. tectorum and examined the effects of extracts from the fermentation broth of representative strains on host spore germination and protonema growth. Sixty-eight endophytic bacteria strains were classified into Bacillus using 16S rDNA sequence analysis, including B. subtilis, B. simplex, B. cereus, B. niacini, B. aryabhattai, B. pumilus, and Bacillus sp. The dominant species was B. subtilis. Host spore germination was not significantly affected by petroleum ether, ethyl acetate, and n-butyl alcohol extracts of B. subtilis DT-1 and B. cereus DT-9 at concentrations of 1 mug/mL, 10 mug/mL, and 100 mug/mL. Host protonema growth was inhibited to differing extents by petroleum ether extract and promoted to differing extents by ethyl acetate and n-butyl alcohol extracts at the three investigated concentrations. These results indicate that growth and development of bryophytes at the protonema stage are closely related to the secondary metabolites of endophytic bacteria. This study provides a valuable insight into the formation of artificial moss-dominated biotic crusts in arid and semi-arid regions.
中文关键词	黄土丘陵区 ; 藓类生物结皮 ; 苔藓植物 ; 对齿藓属 ; 内生细菌 ; 发酵产物
英文关键词	hilly Loess Plateau region moss-dominated biotic crust bryophyte Didymodon endophytic bacteria fermentation product
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:6164900
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236924
作者单位	内蒙古大学生命科学学院, 呼和浩特, 内蒙古 010021, 中国
推荐引用方式 GB/T 7714	麻扬,胡素静,王潇,等. 短叶对齿藓可培养内生细菌的分离及其发酵产物对宿主植物的影响[J],2017,23(6):1028-1034.
APA	麻扬,胡素静,王潇,王丽红,&赵东平.(2017).短叶对齿藓可培养内生细菌的分离及其发酵产物对宿主植物的影响.应用与环境生物学报,23(6),1028-1034.
MLA	麻扬,et al."短叶对齿藓可培养内生细菌的分离及其发酵产物对宿主植物的影响".应用与环境生物学报 23.6(2017):1028-1034.