干旱区生态环境知识资源中心(Arid): 干旱胁迫对栓皮栎种子萌发能力的影响

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	干旱胁迫对栓皮栎种子萌发能力的影响
其他题名	Effect of drought stress on seed germination ability ofQuercus variabilis Bl.
	吴敏; 张文辉; 马闯; 周建云
来源期刊	西北农林科技大学学报. 自然科学版
ISSN	1671-9387
出版年	2017
卷号	45 期号:5 页码:91-100
中文摘要	【目的】揭示栓皮栎种子萌发对干旱环境的适应能力。【方法】采用不同质量浓度的聚乙二醇(PEG-6000)模拟不同程度的干旱胁迫(0.1, 0.3,-0.6, 0.9, 1.2, 1.5 MPa),以蒸馏水为对照,研究栓皮栎种子萌发能力及生理生化指标对干旱胁迫的响应,并对种子萌动、萌芽与出苗的环境水势予以定量分析。【结果】(1)在-0.1 MPa时,栓皮栎种子萌发率、萌发势和胚根长均高于对照,且种子萌发启动时间、萌发指数、活力指数与对照无明显差异;在-0.3~-0.9 MPa时,各项萌发指标值均明显低于对照,但萌发率仍保持在52.95%~20.43%;在-1.2~-1.5 MPa时,种子几乎不能萌发。当环境水势高于-1.2 MPa时,栓皮栎可通过增大根芽比适应干旱环境。(2)栓皮栎种子从萌动到萌芽再到出苗的过程,对环境水分状况逐渐敏感,水势依次为-0.12,-0.08和-0.06 MPa。(3)干旱导致栓皮栎种子内丙二醛含量增加。在-0.1~-0.9 MPa时,3种有机渗透调节物质(脯氨酸、可溶性糖、可溶性蛋白)含量呈现不同的变化,但均显著高于对照;在-1.2~-1.5 MPa时,可溶性糖和可溶性蛋白含量均有不同程度减少,其中可溶性蛋白含量在-1.2 MPa时就开始低于对照,而可溶性糖在-1.5 MPa时才低于对照。在-0.1~-0.6 MPa时,抗氧化酶活性(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX))和抗坏血酸(ASA)含量升高;在-0.9 MPa时,POD活性和ASA含量降低;而-1.2~-1.5 MPa时抑制了 SOD、CAT、POD、APX活性的增加,ASA含量也显著下降。【结论】当环境水势不低于-0.9 MPa时,栓皮栎种子可以通过调节生理功能萌发,但超出其耐受力则不能萌发成苗;水分条件是阻碍黄土高原干旱半干旱地区栓皮栎种群更新与造林成败的因素之一。
英文摘要	【Obective】This paper investigated the adaptive capability of Quercus variabilis Bl. seed to draught stress. 【 Method】 The research investigated the responses of germination, physiological and biochemical indexes of Quercus variabilis Bl. to drought stress simulated by different mass concentrations of polyethylene glycol (PEG)-6000 (-0.1,-0.3,-0. 6,-0.9,-1.2,and-1.5 MPa),and distilled water as control. The environment water potentials of seed radical emergence, plumule emergence and seedling establishment were also quantitatively analyzed.【Result】(1) The seed germination rate, germination vigor and radicle length under-0.1 MPa were insignificantly higher than those under control. These germination parameters under-0.3 to-0.9 MPa were significantly lower than those under control,while the germination rate maintained at 52. 95 %-20.43%. Seeds could hardly germinate under-1.2 to-1.5 MPa. The root/shoot ratio increased to adapt appropriate drought stress when environment water potential was ≥-1.2 MPa. (2) Q. variabilis Bl. seed showed gradually increased sensitivity to moisture environment from the seed germination (-0.12 MPa) to radicles emergency (-0.08 MPa) and sprouting stage(-0.06 MPa). (3) Droug ht induced an increase in malondialdehyde (MDA) content. The contents of three organic osmoregulation substances (free proline, soluble sugar and protein) presented different responses under-0.1 to-0.9 MPa,but all were significantly higher than under control. Soluble sugar and protein reduced under-1.2 to-1.5 MPa. Soluble protein began to decrease to lower than the control under-1.2 MPa,while soluble sugar did not reduce until under-1.5 MPa. Activities of superoxide dismutase(SOD),catalase (CAT), peroxidase (POD) and ascorbate peroxidase ( APX) , and ascorbic acid content(ASA) increased under-0.1 to-0.6 MPa,while the activity of POD and ASA content declined under-0.9 MPa. All antioxidant enzymes activities and ASA content were suppressed under-1.2 to-1.5 MPa.【Conclusion】Q. variabilis Bl. seed could adapt to drought stress through its own biological and physiological changes to sprout seedling under no less than-0.9 MPa drought stress,but seed could not germinate beyond its drought tolerance. The water condition is a key factor restricting Q. variabilis Bl. population natural regeneration and afforestation in arid and semi arid area of the Loess Plateau.
中文关键词	栓皮栎 ; 干旱胁迫 ; 种子萌发 ; 生理生化
英文关键词	Quercus variabilis Bl. drought stress seed germination physiology and biochemistry
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Forestry
WOS研究方向	Forestry
CSCD记录号	CSCD:5975294
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236798
作者单位	吴敏, 西北农林科技大学, 西部环境与生态教育部重点实验室;;桂东南珍稀经济物种保护利用重点实验室培育基地, 杨凌, 陕西 712100, 中国.; 张文辉, 西北农林科技大学, 西部环境与生态教育部重点实验室, 杨凌, 陕西 712100, 中国.; 马闯, 西北农林科技大学, 西部环境与生态教育部重点实验室, 杨凌, 陕西 712100, 中国.; 周建云, 西北农林科技大学, 西部环境与生态教育部重点实验室, 杨凌, 陕西 712100, 中国.
推荐引用方式 GB/T 7714	吴敏,张文辉,马闯,等. 干旱胁迫对栓皮栎种子萌发能力的影响[J]. 西北农林科技大学,2017,45(5):91-100.
APA	吴敏,张文辉,马闯,&周建云.(2017).干旱胁迫对栓皮栎种子萌发能力的影响.西北农林科技大学学报. 自然科学版,45(5),91-100.
MLA	吴敏,et al."干旱胁迫对栓皮栎种子萌发能力的影响".西北农林科技大学学报. 自然科学版 45.5(2017):91-100.