干旱区生态环境知识资源中心(Arid): 基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究

Arid

	基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究
其他题名	Soil Bacteria Diversity in Rhizosphere under Two Types of Vegetation Restoration Based on High Throughput Sequencing
	戴雅婷 1; 闫志坚 2; 解继红 2; 吴洪新 2; 徐林波 2; 侯向阳 1; 高丽 1; 崔艳伟 2
来源期刊	土壤学报
ISSN	0564-3929
出版年	2017
卷号	54 期号:3 页码:735-748
中文摘要	为研究库布齐沙地生态恢复过程中不同植被恢复类型土壤微生物细菌群落结构、多样性的变化特征,以流动沙地为对照,运用高通量测序技术,对自然恢复的油蒿群落、人工种植的中间锦鸡儿群落根际和非根际土壤细菌多样性进行了研究,并分析了土壤理化性质对其分布的影响。结果表明:(1)与流沙对照相比,两种植被恢复类型对细菌多样性产生了正效应,细菌群落丰度、多样性和均匀度明显增加。其中,自然恢复的油蒿群落土壤细菌丰度高于人工种植的中间锦鸡儿群落; (2)变形菌门、酸杆菌门和放线菌门为研究区土壤中的优势细菌类群,其中变形菌门在各样地丰度比例最高,变形菌的4个亚群变化趋势一致,alpha-变形菌相对含量在油蒿和中间锦鸡儿群落根际土壤中明显增加,尤其是自然恢复的油蒿群落根际土壤中alpha-变形菌得到了很好的恢复;(3)土壤有机质、全氮、速效氮、速效钾含量和土壤含水量是影响土壤细菌群落丰度和多样性的主要土壤因子,典型相关分析表明土壤有机质、全氮、全钾、速效钾、速效氮含量对于研究区土壤细菌群落遗传多样性的变化起着重要作用。
英文摘要	【Objective】 Artemisia ordosica and Caragana intermedia are the two widely-distributed and efficient sand-fixing shrubs in the Hobq Desert that have received considerable attention because of the roles they may play in the vegetation restoration. Soil bacteria diversity is an important indicator of the state of soil microbial communities and one indicating progress of ecological restoration. This project is oriented to study changes in community structure and diversity of soil bacteria in the process of vegetation and ecological restoration in the Hobq Desert relative to type of vegetation. 【Method】 In this study, two types of vegetations, naturally restored Artemisia ordosica and artificially planted Caragana intermedia, were chosen for comparison to see effects of the vegetations on community structure and diversity of soil bacteria with a tract of moving sand land as control. The sample plots were set up in 1995 and soil samples were collected from the rhizospheres of A. ordosica and C. intermedia and bulk soil in the 0 ~ 20 cm soil layer and CK in August 2014 for analysis of soil bacteria diversity using the high throughput sequencing method. And relationship between soil bacterial diversity and soil physic-chemical properties, and the effect of soil physical and chemical properties on distribution of bacterial community were also studied through canonical correlation analysis and cononical correspondence analysis.【Result】 (1) Chaol index, Shannon index and ACE index are important indicators of soil bacterial diversity and richness. In terms of Chaol index and Shannon index, the sample plots displayed an order of rhizosphere soil of A. ordosica > rhizosphere soil of C. intermedia > non-rhizosphere soil of A. ordosica > non-rhizosphere soil of C. intermedia > soil of CK, while in terms of ACE index, they followed another order: rhizosphere soil of A. ordosica > rhizosphere soil of C. intermedia > non-rhizosphere soil of C. intermedia > non-rhizosphere soil of A. ordosica > soil of CK. (2) Classification at the phylum level shows the bacterial communities in the samples were mainly composed of Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Gemmatimonadetes, Planctomycetes, Verrucomicrobia, Firmicutes and Chloroflexi, accounting for 88.67% ~ 94.19%. Proteobacteria, Acidobacteria and Actinobacteria were the dominant bacterial groups in the samples, and Proteobacteria was the highest in abundance. The four subgroups under Proteobacteria followed a similar trend in variation. Relative abundance of a-proteobacteria in the rhizosphere and bulk soils under either vegetation exhibited a sharply rising trend, increasing in the rhizosphere of A. ordosica and C. intermedia increased by 106.46% and 70.60% respectively. (3) Soil organic matter, total N, total K, readily available N,readily available K, and soil water are the main soil factors affecting abundance and diversity of soil bacteria communities. Correlation analysis of soil physic-chemical properties with relative abundance of dominant soil bacteria groups shows that the abundance of Proteobacteria was significantly related to soil organic matter, total N,readily available N, readily available P,and readily available K,while the abundance of Acidobacteria was to soil organic matter, total N, readily available N, readily available P and readily available K. Intrestingly, the abundance of Actinobacteria was not so much related to soil factors. Cononical correspondence analysis indicates that soil organic matter, total N,total K, readily available K and readily available N play an important role in triggering variation of genetic diversity of the bacterial communities in the soils.
中文关键词	根际土壤 ; 细菌群落多样性 ; 高通量测序
英文关键词	Rhizosphere soil Bacterial community diversity High throughput sequencing
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6001714
来源机构	中国农业科学院草原研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236768
作者单位	1.中国农业科学院草原研究所, 农业部草地生态与修复治理重点实验室, 呼和浩特, 内蒙古 010010, 中国; 2.中国农业科学院草原研究所, 呼和浩特, 内蒙古 010010, 中国
推荐引用方式 GB/T 7714	戴雅婷,闫志坚,解继红,等. 基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究[J]. 中国农业科学院草原研究所,2017,54(3):735-748.
APA	戴雅婷.,闫志坚.,解继红.,吴洪新.,徐林波.,...&崔艳伟.(2017).基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究.土壤学报,54(3),735-748.
MLA	戴雅婷,et al."基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究".土壤学报 54.3(2017):735-748.