干旱区生态环境知识资源中心(Arid): 4组沙漠绿藻分子生物学鉴定及其生物学特性研究

Arid

	4组沙漠绿藻分子生物学鉴定及其生物学特性研究
其他题名	Morphological and molecular identification of four groups desert green algae
	卡丽比努尔·艾依提; 艾山江; 努尔古丽·热合曼
来源期刊	食品与机械
ISSN	1003-5788
出版年	2017
卷号	33 期号:5 页码:25-29
中文摘要	对从塔克拉玛干沙漠中分离的4组沙漠绿藻进行形态特征鉴定,绘制生长曲线,考察pH对其生长的影响,并进行系统发育分析。形态特征结果显示a组为椭圆形藻,b、c和d组为球状藻。a组沙漠藻最适pH值为6.0,c组的最适pH值以原BG-11培养基为准,b和d组最适pH值为8.0。采用分子生物学18S rDNA序列分析法鉴定出代表性4株沙漠藻的基因序列,结果表明:a组的3YS16①和c组3YS21-1的序列跟Chlamydomonas incerta亲缘关系很近;b组的KXII(2)序列跟Chlorosarcinopsis bastropiensis亲缘关系很近;d组的YS2-3的序列跟Chlorosarcinopsis communis亲缘关系很近。该研究确定了4组藻的分类地位和最佳生长pH条件,可为沙漠绿藻分子生物学和食品应用方面的研究提供理论依据。
英文摘要	Four groups of algas, isolated from the Taklimakan desert, were identified by morphological characters in this study. The pH effect of the growth of algae is analyzed according to the growth curve, and the phylogenetic tree was pictured. It shows that the group a belongs to the oval algae, and group b, c and d belong to the spherical algae. The optimum suitable pH values for the group a desert algae was 6.0, 8.0 for the group b and d; the same value of original BG-11 for the group c. Four representative strains of desert algae gene sequences were identified by using the 18S rDNA sequence analysis method. It shows that the strain of 3YS16① (group a) and the strain of 3YS21-1 (group c) have close genetic relationship with the Chlamydomonas incerta; the strain of KXII(2) (group b) has close genetic relationship with the Chlorosarcinopsis bastropiensi; the Strain of YS2-3 (group d) has close genetic relationship with Chlorosarcinopsis communis. This study determined the classification status of four groups of algae and the optimal pH growth conditions, and provided theoretical basis to the molecular biology studies of desert algae and applications in food industry.
中文关键词	沙漠绿藻 ; 形态特性
英文关键词	18S rDNA Desert algae 18S rDNA Morphological Characteristics
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:6018154
来源机构	新疆师范大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236603
作者单位	新疆师范大学生命科学学院, 新疆特殊环境物种保护与调控生物学实验室, 乌鲁木齐, 新疆 830054, 中国
推荐引用方式 GB/T 7714	卡丽比努尔·艾依提,艾山江,努尔古丽·热合曼. 4组沙漠绿藻分子生物学鉴定及其生物学特性研究[J]. 新疆师范大学,2017,33(5):25-29.
APA	卡丽比努尔·艾依提,艾山江,&努尔古丽·热合曼.(2017).4组沙漠绿藻分子生物学鉴定及其生物学特性研究.食品与机械,33(5),25-29.
MLA	卡丽比努尔·艾依提,et al."4组沙漠绿藻分子生物学鉴定及其生物学特性研究".食品与机械 33.5(2017):25-29.