干旱区生态环境知识资源中心(Arid): 荒漠昆虫小胸鳖甲抗冻蛋白MpAFP698对酿酒酵母的低温保护作用

Arid

	荒漠昆虫小胸鳖甲抗冻蛋白MpAFP698对酿酒酵母的低温保护作用
其他题名	Cryoprotective effect of antifreeze protein MpAFP698 from the desertinsect Microdera punctipennis on yeast Saccharomyces cerevisiae
	张凤娟; 孙琳洁; 李素丽; 马纪
来源期刊	食品与发酵工业
ISSN	0253-990X
出版年	2017
卷号	43 期号:5 页码:37-42
中文摘要	昆虫抗冻蛋白作为天然无毒副作用的低温和冷冻保护剂,在发酵菌种的保藏方面具有很好应用前景。为了验证荒漠昆虫小胸鳖甲(Microdera punctipennis)抗冻蛋白MpAFP698对酵母菌的抗冻和抗低温保护效果,对MpAFP698抗冻蛋白进行原核表达和纯化,获得了具有活性的融合抗冻蛋白MBP-MpAFP698。将质量浓度为1、10、30 mug/mL的MBP-MpAFP698添加到酿酒酵母(Saccharomyces cerevisiae)中,在-7和-20℃冷冻处理酵母菌液不同时间;以10 mug/mL的抗冻蛋白在4、0、-50℃处理酵母菌液,在-20 ℃反复冻融处理,观察菌落形态并统计菌种存活率。结果表明,添加抗冻蛋白组的酵母存活率显著优于未加抗冻蛋白的对照组,1 mug/mL就有保护效果,10 mug/mL保护效果最好,说明昆虫抗冻蛋白能以极低的浓度发挥低温保护作用。
英文摘要	Antifreeze proteins as non-toxic cryoprotective agents have extensive prospect in fermentation strain preservation. To test the cryoprotective effect of antifreeze protein MpAFP698 from the desert beetle Microdera punctipennis on S. cerevisiae, antifreeze protein MpAFP698 was prokaryotic expressed and purified. Fusion protein MBP-MpAFP698 with high activity was obtained. 1,10,30 mug/mL of MBP-MpAFP698 were added to S. cerevisiae and then treated at - 7 and - 20 ℃ for different time lengths; in other experiments yeast cells were treated at 4,0, -50 ℃ for different time lengths with 10 mug/mL MpAFP698 as well as repeated freeze-thaw at -20 ℃. The morphology and survival rate of the yeast cells after these treatments were observed or calculated. The results showed that the survival rate of S. cerevisiae with the protection of MBP-MpAFP698 were significantly better than the control group without MBP-MpAFP698. Even the concentration of 1 mug/mL showed protective effect, and 10 mug/mL showed the best protective effect, indicating that insect antifreeze protein can function as cyroprotective agent at very low concentrations. This result lay a foundation for the development of efficient protective agent at low temperatures.
中文关键词	荒漠昆虫抗冻蛋白 ; 酿酒酵母 ; 抗冻保护 ; 细胞存活
英文关键词	desert insect antifreeze protein Saccharomyces cerevisiae antifreeze protection cell survival
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENGINEERING CHEMICAL
WOS研究方向	Engineering
CSCD记录号	CSCD:6000989
来源机构	新疆大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236602
作者单位	新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐, 新疆 830046, 中国
推荐引用方式 GB/T 7714	张凤娟,孙琳洁,李素丽,等. 荒漠昆虫小胸鳖甲抗冻蛋白MpAFP698对酿酒酵母的低温保护作用[J]. 新疆大学,2017,43(5):37-42.
APA	张凤娟,孙琳洁,李素丽,&马纪.(2017).荒漠昆虫小胸鳖甲抗冻蛋白MpAFP698对酿酒酵母的低温保护作用.食品与发酵工业,43(5),37-42.
MLA	张凤娟,et al."荒漠昆虫小胸鳖甲抗冻蛋白MpAFP698对酿酒酵母的低温保护作用".食品与发酵工业 43.5(2017):37-42.