干旱区生态环境知识资源中心(Arid): 基于通过型固相萃取-高效液相色谱-串联质谱法同时测定水产品中的氯霉素和红霉素

Arid

	基于通过型固相萃取-高效液相色谱-串联质谱法同时测定水产品中的氯霉素和红霉素
其他题名	Simultaneous Determination of Chloramphenicol and Erythromycin Residues in Aquatic Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry with Oasis PRiME HLB Solid Phase Extraction Cleanup
	柯庆青; 李诗言; 周凡; 郑重莺; 贝亦江; 王鼎南; 王扬
来源期刊	食品科学
ISSN	1002-6630
出版年	2017
卷号	38 期号:24 页码:241-246
中文摘要	建立通过型固相萃取-高效液相色谱-串联质谱同时快速测定水产品中氯霉素和红霉素的分析方法。样品通过乙腈提取,Oasis PRiME HLB小柱一步法净化,采用高效液相色谱-串联质谱仪多反应监测扫描模式检测和同位素内标定量。检测水产品中的氯霉素和红霉素定量限分别为0.1 mug/kg和0.5 mug/kg。不同水产品(鱼、虾和蟹)在3个添加水平的平均回收率在86.7%~102.6%之间,相对标准偏差小于5%(n=6)。本方法简单易行、灵敏度较高,在日常检测方面存在一定优势。
英文摘要	A rapid method was developed for the simultaneous determination of chloramphenicol and erythromycin residues in aquatic products by high performance liquid chromatography-tandem mass spectrometry with solid phase extraction (SPE). Samples were extracted with acetonitrile and the extract was then purified by an Oasis PRiME HLB SPE cartridge. The analytes were detected in the multi-reaction monitoring (MRM)mode and quantified by the isotope internal standard method. The limits of quantification (LOQ)for chloramphenicol and erythromycin were 0.1 and 0.5 mug/kg, respectively. The average recoveries of the 2 analytes ranged from 86.7% to 102.6% with relative standard deviations of less than 5% in aquatic products (fish, shrimp and crab)at three spiked levels (n = 6). This proposed method was advantageous in routine detection of antibiotic residues in aquatic products for its simplicity and high sensibility.
中文关键词	固相萃取 ; 高效液相色谱-串联质谱 ; 水产品 ; 氯霉素 ; 红霉素
英文关键词	solid phase extraction high performance liquid chromatography-tandem mass spectrometry aquatic products chloramphenicol erythromycin
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6129201
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236601
作者单位	浙江省水产质量检测中心, 杭州, 浙江 310023, 中国
推荐引用方式 GB/T 7714	柯庆青,李诗言,周凡,等. 基于通过型固相萃取-高效液相色谱-串联质谱法同时测定水产品中的氯霉素和红霉素[J],2017,38(24):241-246.
APA	柯庆青.,李诗言.,周凡.,郑重莺.,贝亦江.,...&王扬.(2017).基于通过型固相萃取-高效液相色谱-串联质谱法同时测定水产品中的氯霉素和红霉素.食品科学,38(24),241-246.
MLA	柯庆青,et al."基于通过型固相萃取-高效液相色谱-串联质谱法同时测定水产品中的氯霉素和红霉素".食品科学 38.24(2017):241-246.