干旱区生态环境知识资源中心(Arid): 羊奶及奶粉中27种beta -受体激动剂类药物残留UPLC-MS /MS检测方法

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	羊奶及奶粉中27种beta -受体激动剂类药物残留UPLC-MS /MS检测方法
其他题名	Simultaneous determination of twenty-seven beta-agonists residues in goat milk and powder using ultra performance liquid chromatography tandem mass spectrometry
	刘洪斌 1; 李颖 1; 姚喜梅 2; 于雷 1; 蔡英华 1
来源期刊	食品工业科技
ISSN	1002-0306
出版年	2017
卷号	38 期号:23 页码:214-220
中文摘要	采用超高效液相色谱串联质谱建立羊奶及奶粉中吡布特罗、丙卡特罗、瑞普特罗、克伦普罗、奥达特罗等27种 beta-受体激动剂类药物残留检测方法。样品经过1%甲酸乙腈提取、Oasis PRiME HLB柱净化后,经Acquity UPLC BEH C_(18)( 100 mm *2.1 mm,1.7 mum)分离,以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,多通道MRM信号采集模式, 27种beta-受体激动剂类药物能在10.5 min内出峰完好,在1.0~100.0 mug /L浓度范围内,27种beta-受体激动剂类药物线性良好,相关系数均在0.993以上;羊奶中最低定量限均低于0.5 mug /kg,通过0.5、2.5、5.0 mug /kg三个浓度的加标回收实验表明,回收率为65.7%~119%,批内变异系数为0.79%~10.4%,批间变异系数为2.13%~15.7%。奶粉中最低定量限均低于2.0 mug /kg,通过2.0、10.0、20.0 mug /kg三个浓度的加标回收实验表明,回收率为60.5% ~109%,批内变异系数为1.91%~12.4%,批间变异系数为3.06%~17.2%。所建立方法为一种高通量检测羊奶及奶粉中beta-受体激动剂类药物残留确证分析方法。
英文摘要	A multiresidue method based on ultra performance liquid chromatography tandem mass spectrometry has been developed for the determination of twenty- seven beta- agonists( pyrbuterol,procaterol,reproterol,clenproperol,olodaterol.et al) residues in goat milk and powder.In this method,analytes were extracted by acidified acetonitrile,and purified by Oasis PRiME HLB SPE column.twenty- seven beta- agonists were analyzed by Acquity UPLC BEH C_(18) column( 100 mm *2.1 mm,1.7 mum) using methanol and 0.1% formic acid as the mobile phases.The signals are acquired through the multi-channel MRM mode.All analytes could be well-separated by a gradient program during 10.5 minutes.The calibration curves of twenty-seven beta-agonists were linear in a concentration range of 1.0~100.0 mug /L( r >0.993),and the limits of quantitation were all less than 0.5 mug /kg in goat milk.The recoveries of 0.5,2.5,5.0 mug /kg fortified samples ranged from 65.7%~119%,with the intra-assay coefficient of variation was 0.79%~10.4% and the inter-assay coefficient of variation was 2.13%~15.7%.The limits of quantitation were all less than 2.0 mug /kg in milk powder.The recoveries of 2.0,10.0,20.0 mug /kg fortified samples ranged from 60.5% ~109%, with the intra-assay coefficient of variation was 1.91%~12.4% and the inter-assay coefficient of variation was 3.06%~17.2%. The method was established as a high-throughput method for confirmation of beta-agonists residues in goat milk and powder.
中文关键词	羊奶 ; 奶粉 ; beta-受体激动剂类 ; 残留
英文关键词	UPLC-MS /MS goat milk milk powder beta-agonists residues UPLC-MS /MS
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:6128964
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236599
作者单位	1.中国动物疫病预防控制中心, 北京 102609, 中国; 2.中国标准化协会, 北京 100048, 中国
推荐引用方式 GB/T 7714	刘洪斌,李颖,姚喜梅,等. 羊奶及奶粉中27种beta -受体激动剂类药物残留UPLC-MS /MS检测方法[J],2017,38(23):214-220.
APA	刘洪斌,李颖,姚喜梅,于雷,&蔡英华.(2017).羊奶及奶粉中27种beta -受体激动剂类药物残留UPLC-MS /MS检测方法.食品工业科技,38(23),214-220.
MLA	刘洪斌,et al."羊奶及奶粉中27种beta -受体激动剂类药物残留UPLC-MS /MS检测方法".食品工业科技 38.23(2017):214-220.