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	沙漠植物花花柴幼苗对高温耐受性评价
其他题名	An Evaluation on Heat Tolerance of a Desert Plant Karelinia caspia Seedlings
	王彦芹 1; 石新建 2; 李志军 1
来源期刊	生物技术通报
ISSN	1002-5464
出版年	2017
卷号	33 期号:4 页码:157-163
中文摘要	为开发利用沙漠植物花花柴的耐高温特性,就花花柴对高温耐受性进行了探测。采用不同的温度(40、45和50℃)处理花花柴幼苗,分别在不同温度的不同处理时间段用传统方法测定了花花柴幼苗的相对电导率和MDA含量、SOD、POD和CAT的活性变化,台盼蓝组化染色面积,并利用隶属函数法综合分析了这几个指标对评价花花柴对高温耐受性的可靠性。结果显示,(1)花花柴幼苗在40℃条件下,随着胁迫时间延长,叶片相对电导率和MDA含量呈先升高后降低的趋势,其保护酶系统的活性随处理时间的延长逐渐升高,台盼蓝染色几乎无着色。(2)在45℃条件下,处理2-8 h时间段,各项生理指标变化不显著,而在处理12 h以后,相对电导率、MDA含量显著增加,保护酶活性显著降低;台盼蓝染色面积随着处理时间的延长逐渐增大。(3)在50℃处理时,花花柴幼苗叶片的MDA含量、相对电导率逐渐升高,且呈显著水平,其保护酶活性在处理1 h时达到最高,之后显著降低;台盼蓝染色面积随着处理时间的延长逐渐增大、着色变深。通过隶属函数法综合分析表明这5个指标可作为花花柴耐高温性的评价指标,且花花柴对40℃不敏感,对45℃可耐受8 h左右,对50℃能耐受1 h左右,表明花花柴对高温具有极强的耐受性。
英文摘要	In order to explore and utilize the feature of heat tolerance of a desert plant Karelinia caspia,its heat tolerance during K. caspia seedling was evaluated. The 5 physiological indexes,relative conductivity,MDA content,activity variation of SOD,POD and CAT,as well as trypan blue staining in the K. caspia seedling leaves were measured at varied timeslots under different temperature treatments (40,45 and 50℃)by traditional methods. The reliability of using these indexes in the evaluation of the heat tolerance of K. caspia was comprehensively analyzed by subordinate function method. Results showed that :(1)Under 40℃,the relative conductivity and MDA content tended to increase during early stage of treatment and then reduce in K. caspia seedling with the stress time increasing. The activities of SOD, POD and CAT rose with treatment time longing. There was no coloration on the leaves of K. caspia by trypan blue staining.(2)Under 45℃, each index remained stable at 2-8 h after treatment. The MDA content and relative conductivity rose sharply at 12 h after treatment,but the enzymatic activities declined. With the extension treatment time,the relative coloration area of K. caspia leaves by trypan blue staining enlarged gradually.(3)Under 50℃,the MDA content and relative conductivity rose gradually to the significant level. The activities of SOD,POD, and CAT reached peak at 1 h after treatment,but rapidly decreased as treating time extending. With the extension of treatment time,the relative coloration area of K. caspia leaves by trypan blue staining enlarged gradually,and the color became heavily. The results of subordinate function method showed that these 5 physiological indexes could be used as the indicators of evaluating the feature of heat tolerance in K. caspia. In addition,the K. caspia was not susceptible to 40℃,and the tolerant time was 8 h under 45℃ and 1 h under 50℃,indicating that K. caspia is a highly tolerant plant to high temperature.
中文关键词	花花柴 ; 高温胁迫 ; 高温耐受
英文关键词	Karelinia caspia L. high temperature stress tolerance to heat stress
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:5965642
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236593
作者单位	1.塔里木大学生命科学学院, 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室, 阿拉尔, 新疆 843300, 中国; 2.塔里木大学植物科学学院, 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室, 阿拉尔, 新疆 843300, 中国
推荐引用方式 GB/T 7714	王彦芹,石新建,李志军. 沙漠植物花花柴幼苗对高温耐受性评价[J],2017,33(4):157-163.
APA	王彦芹,石新建,&李志军.(2017).沙漠植物花花柴幼苗对高温耐受性评价.生物技术通报,33(4),157-163.
MLA	王彦芹,et al."沙漠植物花花柴幼苗对高温耐受性评价".生物技术通报 33.4(2017):157-163.