干旱区生态环境知识资源中心(Arid): 采用稳定碳同位素法分析白羊草在不同干旱胁迫下的水分利用效率

Arid

	采用稳定碳同位素法分析白羊草在不同干旱胁迫下的水分利用效率
其他题名	Effects of drought stress on Bothriochloa ischaemum water-use efficiency based on stable carbon isotope
	刘莹; 李鹏; 沈冰; 冯朝红; 刘琦; 张祎
来源期刊	生态学报
ISSN	1000-0933
出版年	2017
卷号	37 期号:9 页码:3055-3064
中文摘要	本研究以黄土高原乡土草种白羊草(betaothriochloa ischaemum (L.) Keng.)为研究对象,采用盆栽控制实验,比较白羊草在3个水分处理(CK80%FC、MS60%FC和SS 40%FC)下的生物量积累和分配模式、瞬时水分利用效率(WUE)、不同部位(新叶、老叶、茎、细根、粗根)的稳定碳同位素组成(delta~(13)C)和碳同位素分辨率(?~(13)C)及其相互关系,以及干旱胁迫下影响水分利用效率的主导环境因子。结果表明:1)重度干旱胁迫显著降低植物整体生物量,显著增加根冠比和细根生物量比例;2)随着干旱胁迫加剧,白羊草各器官的delta~(13)C均呈上升趋势,?~(13)C呈减小趋势,SS处理不同器官delta~(13)C和?~(13)C没有显著差异,CK和MS处理的各器官delta~(13)C均值表现分别为细根>粗根>老叶>新叶>茎、细根>新叶>老叶>粗根>茎,CK和MS处理Delta~(13)C的值总体呈根<叶<茎。3)新叶的delta~(13)C_(NL)和?~(13)C_(NL)与WUE的相关系数均最大,说明利用稳定碳同位素方法测定白羊草水分利用效率具有可行性。4)不同水分处理的WUE的主导影响因子不同,CK、MS、SS水分处理WUE分别受到叶面温度、大气水汽压亏缺和空气温度的影响最大。为采用稳定碳同位素方法指示白羊草水分利用效率可行性及阐明植物的胁迫响应机制提供理论依据。
英文摘要	Owing to low precipitation and high inter-annual variability, the Loess hilly and valley region experiences frequent drought, which significantly affects plant growth. Studying the response mechanism of plants to water stress is important with respect to improving water-use efficiency (WUE) in this arid and semi-arid area. In the present research, Bothriochloa ischaemum (L.) Keng.,a typical native species in the Loess hilly-gully region,was selected to investigate biomass accumulation and allocation,instantaneous WUE,stable carbon isotope composition (delta~(13)C),and stable carbon isotope discrimination (Delta~(13)C) in different organs under three water regimes: control [ CK, 80% field capacity (FC)], mild water stress (MS,60% FC),and severe stress (SS,40% FC). Photosynthetic parameters of B. ischaemum were measured at 09: 00-11 : 00 on sunny windless days (Aug 3, 10,and 13, 2014), using a portable photosynthesis determination system (Li-6400; Li-Cor, USA). On Nov 11,when the experiment ended, the new leaf, old leaf, stem, fine root, and coarse root parts of seedlings were sampled carefully and weighed (with accuracy 0.001 g) after drying at 65℃. The root-to-shoot ratio was then calculated as the ratio of the belowground dry biomass to aboveground dry biomass. The delta~(13)C of different plant parts was measured using a CCIA-36d-EP Carbon isotope ratio mass spectrometer (Los Gatos Research, USA). The Delta~(13)C of different plant parts was calculated based on delta~(13)C and the carbon isotope ratio of atmospheric CO_2. Many environmental factors that might affect WUE were analyzed to explore the response mechanisms of B. ischaemum under drought conditions. The following results were obtained. (1) The total biomass of B. ischaemum was significantly decreased under the SS condition, whereas the root-to-shoot ratio and fine root biomass were significantly increased under this water regime. (2) The delta~(13)C and Delta~(13)C in different plant parts showed different trends. Drought stress led to an increase in the delta~(13)C value and decrease in the Delta~(13)C value. There was no significant difference in the delta~(13)C and Delta~(13)C of different plant parts under the SS condition. The delta~(13)C values under CK and MS conditions were in the order of fine root > coarse root > old leaf > new leaf > stem, and fine root > new leaf > old leaf > coarse root > stem, respectively. Furthermore, the Delta~(13)C values under CK and MS conditions were all in the order of root < leaf
中文关键词	白羊草 ; 稳定碳同位素 ; 生物量 ; 水分利用效率 ; 干旱胁迫
英文关键词	Bothriochloa ischaemum Stable carbon isotope Biomass Water-use efficiency Drought stress
语种	中文
国家	中国
收录类别	CSCD
WOS类目	BIOLOGY
WOS研究方向	Life Sciences & Biomedicine - Other Topics
CSCD记录号	CSCD:5984199
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236472
作者单位	西安理工大学, 陕西省西北旱区生态水利工程国家重点实验室培育基地, 西安, 陕西 710048, 中国
推荐引用方式 GB/T 7714	刘莹,李鹏,沈冰,等. 采用稳定碳同位素法分析白羊草在不同干旱胁迫下的水分利用效率[J],2017,37(9):3055-3064.
APA	刘莹,李鹏,沈冰,冯朝红,刘琦,&张祎.(2017).采用稳定碳同位素法分析白羊草在不同干旱胁迫下的水分利用效率.生态学报,37(9),3055-3064.
MLA	刘莹,et al."采用稳定碳同位素法分析白羊草在不同干旱胁迫下的水分利用效率".生态学报 37.9(2017):3055-3064.