干旱区生态环境知识资源中心(Arid): 固相萃取-高效液相色谱法同时测定牛组织中三氮脒和氯化氮氨菲啶残留

Arid

	固相萃取-高效液相色谱法同时测定牛组织中三氮脒和氯化氮氨菲啶残留
其他题名	Simultaneous determination of diminazene aceturate and isometamidium chloride residues in cattle tissues by high performance liquid chromatography with solid-phase extraction
	周雨卉; 曾振灵; 余静贤; 刘戎; 潘丽花; 钟秋玉; 陈红
来源期刊	色谱
ISSN	1000-8713
出版年	2017
卷号	35 期号:9 页码:995-1002
中文摘要	建立了固相萃取-高效液相色谱同时检测牛组织中三氮脒(DIM)和氯化氮氨菲啶(ISM)残留的分析方法。针对不同的组织样品采用不同比例的水-乙腈溶液提取药物,结合Oasis WCX固相萃取小柱净化富集。采用Spherisorb CN柱(250 mm*4.6 mm, 5 mum)对药物进行分离,以乙腈和0.05 mol/L甲酸铵溶液(pH 2.4)为流动相,梯度洗脱,紫外检测波长为380 nm。牛组织中DIM和ISM的检出限(S/N≥3)为0.01 mg/kg,定量限(S/N≥10)为0.025 mg/kg。牛组织中DIM和ISM在各自的线性范围内线性关系良好(r≥0.9993)。DIM和ISM在低、中、高添加水平下的回收率为82.2%~97.6%,日内精密度和日间精密度分别为0.3%~5.2%(n=5)和1.3%~5.2%(n=15)。结果表明,建立的方法重复性好,灵敏度高,操作简便,适用于牛组织中DIM和ISM的残留检测。
英文摘要	A method for the simultaneous determination of trypanocidal diminazene aceturate (DIM) and isometamidium chloride (ISM) that containing benzamidine groups in cattle tissues was developed by high performance liquid chromatography (HPLC) with solid-phase extraction (SPE). The tissue samples were extracted with different proportions of water-acetonitrile, then were cleaned up by Oasis WCX cartridges. DIM and ISM were separated by HPLC with a Spherisorb CN column (250 mm*4.6 mm, 5 mum). Acetonitrile-0.05 mol/L ammonium formate solution (pH 2.4) was used as mobile phases with gradient elution. The detection wavelength of UV was set at 380 nm. The limits of detection (LODs) and the limits of quantification (LOQs) of DIM and ISM in cattle tissues were 0.01 mg/kg and 0.025 mg/kg, respectively. The correlation coefficients (r) of DIM and ISM in cattle tissues were not less than 0.9993. The average recoveries of DIM and ISM at three spiked levels were 82.2%-97.6% with the intra-day relative standard derivations (RSDs) of 0.3%-5.2% (n=5) and inter-day RSDs of 1.3%-5.2% (n=15). The method was successfully applied to the analysis of DIM and ISM in cattle tissues. The method is rapid, sensitive and repeatable for the determination of diminazene aceturate and isometamidium chloride in cattle tissues.
中文关键词	高效液相色谱 ; 固相萃取 ; 多残留检测 ; 三氮脒 ; 氯化氮氨菲啶 ; 牛组织
英文关键词	high performance liquid chromatography (HPLC) solid-phase extraction (SPE) multi-residue determination diminazene aceturate (DIM) isometamidium chloride (ISM) cattle tissues
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6075303
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236384
作者单位	华南农业大学兽医学院, 国家兽药残留基准实验室, 广州, 广东 510642, 中国
推荐引用方式 GB/T 7714	周雨卉,曾振灵,余静贤,等. 固相萃取-高效液相色谱法同时测定牛组织中三氮脒和氯化氮氨菲啶残留[J],2017,35(9):995-1002.
APA	周雨卉.,曾振灵.,余静贤.,刘戎.,潘丽花.,...&陈红.(2017).固相萃取-高效液相色谱法同时测定牛组织中三氮脒和氯化氮氨菲啶残留.色谱,35(9),995-1002.
MLA	周雨卉,et al."固相萃取-高效液相色谱法同时测定牛组织中三氮脒和氯化氮氨菲啶残留".色谱 35.9(2017):995-1002.